Creatine Phosphate

Lamprecht, Walther; Stein, Philipp; Heinz, Fritz; Weisser, Herwig

doi:10.1016/b978-0-12-091304-6.50028-8

Cited by 157 publications

(53 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The supernatant of the homogenates was neutralized with a solution of 2N KOH, 0.4 mol/l imidazole, and 0.4 mol/l KCl and then centrifuged at 14,000g at -9°C. The supernatant was collected and analyzed enzymatically for ATP and creatine phosphate (27).…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Increases Glucose Uptake Through a Mechanism That Is Distinct From the Insulin and Contraction Pathways in Rat Skeletal Muscle

et al. 2001

View full text Add to dashboard Cite

Insulin, contraction, and the nitric oxide (NO) donor, sodium nitroprusside (SNP), all increase glucose transport in skeletal muscle. Some reports suggest that NO is a critical mediator of insulin-and/or contraction-stimulated transport. To determine if the mechanism leading to NO-stimulated glucose uptake is similar to the insulin-or contraction-dependent signaling pathways, isolated soleus and extensor digitorum longus (EDL) muscles from rats were treated with various combinations of SNP (maximum 10 mmol/l), insulin (maximum 50 mU/ml), electrical stimulation to produce contractions (maximum 10 min), wortmannin (100 nmol/l), and/or the NO synthase (NOS) inhibitor N G -monomethyl-L-arginine (L-NMMA) (0.1 mmol/l). The combinations of SNP plus insulin and SNP plus contraction both had fully additive effects on 2-deoxyglucose uptake. Wortmannin completely inhibited insulinstimulated glucose transport and only slightly inhibited SNP-stimulated 2-deoxyglucose uptake, whereas L-NMMA did not inhibit contraction-stimulated 2-deoxyglucose uptake. SNP significantly increased the activity of the ␣1 catalytic subunit of 5AMP-activated protein kinase (AMPK), a signaling molecule that has been implicated in mediating glucose transport in fuel-depleted cells. Addition of the NOS inhibitor N G -nitro-L-arginine methyl ester (L-NAME) (1 mg/ml) to the drinking water of rats for 2 days failed to affect the increase in muscle 2-deoxyglucose uptake in response to treadmill exercise. These data suggest that NO stimulates glucose uptake through a mechanism that is distinct from both the insulin and contraction signaling pathways.

show abstract

Section: Methodsmentioning

confidence: 99%

Nitric Oxide Increases Glucose Uptake Through a Mechanism That Is Distinct From the Insulin and Contraction Pathways in Rat Skeletal Muscle

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Briefly, the incorporation of the glycosyl moiety of glucose-1-phosphate into glycogen was measured in the presence and absence of 3 mM 5'-AMP. ATP and creatine phosphate (CP) were measured by spectrophotometric methods (Lamprecht and Stein, 1963).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Indirect and direct effects of the divalent cation ionophore A23187 on guinea pig and rat ventricular myocardium.

Lindemann¹,

Besch²,

Watanabe³

1979

Circulation Research

View full text Add to dashboard Cite

SUMMARY The contractile and biochemical effects of the divalent cation ionophore A23187 were studied in isolated perfused guinea pig and rat ventricles. A23187 increased both cyclic adenosine 3':5'-monophosphate (cyclic AMP) and cyclic guanosine 3':5'-monophosphate (cyclic GMP) concentrations in guinea pig ventricles. Associated with these changes in nucleotide content were positive inotropic effects and activation of giycogen phosphorylase. Pretreatment of «nirrmln with reserpine to deplete endogenous catecholamine stores or pretreatment of hearts with propranolol failed to alter these ionophore-induced change*. In contrast, blockade of histamine (Hi) receptors with metiamide completely abolished the positive inotropic effects and activation of giycogen phosphorylase induced by A23187. In these metiamide-treated hearts, the ionophore-induced increase in cyclic AMP concentration was significantly attenuated, whereas the increase in cyclic GMP content persisted. A23187 produced a negative inotropic effect in hearts taken from rats treated with reserpine. In these rat hearts, cyclic AMP levels and giycogen phosphorylase activity were unchanged, whereas cyclic GMP concentrations were markedly increased. These results indicate that A23187 produces both indirect and direct effects on guinea pig ventricles. The indirect effects are mediated primarily by histamine, presumably released from tissue mast cells by the ionophore. Histamine in turn increases cyclic AMP levels, which leads to activation of phosphorylase and positive inotropic effects. The direct biochemical effect of A23187 is an increase in cyclic GMP concentrations. Ore Rea 44: [473][474][475][476][477][478][479][480][481][482] 1979

show abstract

“…Glycogen concentration was determined as glycosyl units after hydrolysis with α-amyloglucosidase and correction for glucose concentration in the extract to the method of Keppler & Decker (1974). Glucose-6-phosphate and creatine phosphate were determined in perchloric acid extracts according to Lamprecht & Stein (1965).…”

Section: Methodsmentioning

confidence: 99%

Effects of short and extended fasting periods and cattle breed on glycogenolysis, sarcomere shortening and Warner-Bratzler shear force

O’Neill¹,

Webb²,

Frylinck³

et al. 2018

SA J. An. Sci.

View full text Add to dashboard Cite

The effects of short (three hours) and extended (24 hours) feed withdrawal periods and three cattle breeds on muscle energy metabolism, sarcomere length, and meat quality were investigated. Brahman (Br), Nguni (Ng), and Simmental (Sm) bulls were subjected to ante-mortem feed withdrawal of three hours (Br3, n = 10; Ng3, n = 10; and Sm3, n = 10) or 24 hours (Br24, n = 10; Ng24, n = 10; and Sm24, n = 10). M. longissimus was used as the reference muscle for sampling. pH, and temperature was recorded at 1, 2, 3, 6, 9, and 24 hours post mortem. Samples for energy metabolites were removed at 3, 6, 9 and 24 hours post mortem. Glycogen, glucose-6-phosphate and creatine phosphate concentration were determined at each time interval as glycosyl units after hydrolysis. Samples to determine sarcomere length were removed at one and three days post mortem. Homogenates of the samples were placed under a 31,000 magnification microscope and sarcomere lengths were measured. Warner-Bratzler shear force (WBSF) was measured with an Instron meter at 1, 7 and 14 days post mortem. Glycogen was lower for Br24 and Ng24. There were no differences in glucose-6-phosphate, rate of creatine phosphate decline, average sarcomere length, or WBSF for Br24 and Ng24 compared with Br3 and Ng3. There were no differences for WBSF between Br3, Ng3 and Sm3. Glycogen concentration was higher for Sm24 compared with Sm3; glucose-6-phosphate was lower for Sm24 compared with Sm3; and the rate of creatine phosphate decline was higher for Sm24 compared with Sm3. Average sarcomere length was shorter and WBSF was higher for Sm24 compared with Sm3. The effect of prolonged ante-mortem feed withdrawal on tenderness is breed specific. Warner-Bratzler shear force was affected significantly by an extended feed withdrawal period in Simmental cattle only.

show abstract

Creatine Phosphate

Cited by 157 publications

References 15 publications

Nitric Oxide Increases Glucose Uptake Through a Mechanism That Is Distinct From the Insulin and Contraction Pathways in Rat Skeletal Muscle

Nitric Oxide Increases Glucose Uptake Through a Mechanism That Is Distinct From the Insulin and Contraction Pathways in Rat Skeletal Muscle

Indirect and direct effects of the divalent cation ionophore A23187 on guinea pig and rat ventricular myocardium.

Effects of short and extended fasting periods and cattle breed on glycogenolysis, sarcomere shortening and Warner-Bratzler shear force

Contact Info

Product

Resources

About