Creatine kinase rate constant in the human heart measured with 3<scp>D</scp>‐localization at 7 tesla

Clarke, William T.; Robson, Matthew D; Neubauer, Stefan; Rodgers, Christopher T.

doi:10.1002/mrm.26357

Cited by 17 publications

(28 citation statements)

References 43 publications

(97 reference statements)

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“…Myocardial k f CK measured in the prone position further validated the new implementation of TRiST with all cardiac slices in 10 subjects giving 0.29 ± 0.09 s −1 and k f CK from only the most apical voxel for each subject giving a mean of 0.32 ± 0.15 s −1 , although the SD of this measurement is double that reported in the literature (Table ). In the supine position, the measured k f CK throughout this study is much lower than the paired prone estimate, the literature estimate of 0.32 s −1 , and our own 7 T k f CK estimate (0.35 ± 0.05 s −1 ) . It is therefore likely that the absolute value of k f CK measured in a supine position is an underestimate.…”

Section: Discussioncontrasting

confidence: 65%

“…In the supine position, the measured k f CK throughout this study is much lower than the paired prone estimate, the literature estimate of 0.32 s −1 , and our own 7 T k f CK estimate (0.35 ± 0.05 s −1 ). 9 It is therefore likely that the absolute value of k f CK measured in a supine position is an underestimate. However, simulations of the effect of B 0 variation during respiratory and cardiac cycles and correlation with biopsy-measured CK activity in 25 patients indicate that despite the low absolute value of supine MRS-measured k f CK , trends in our measured k f CK values are still meaningful, that is, increases or decreases in measured k f CK correspond to real increases or decreases.…”

Section: Discussionmentioning

confidence: 99%

“…The 3 T TRiST protocol offers an established measurement technique which can be included in a larger cardiac 1 H MR protocol on the same scanner. While 7 T 31 P‐MRS has been used for 3D‐localised k f CK measurements, the existing published methods have long duration and the higher field strength restricts the recruitment of subjects who have undergone surgical procedures …”

Section: Introductionmentioning

confidence: 99%

“…While 7 T 31 P-MRS has been used for 3D-localised k f CK measurements, the existing published methods have long duration and the higher field strength restricts the recruitment of subjects who have undergone surgical procedures. 9 An increase in myocardial work can be reliably maintained, without physical exercise (and therefore motion), by administering dobutamine intravenously. However, the duration of intravenous infusion should be kept to a minimum length.…”

Section: Introductionmentioning

confidence: 99%

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Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T

et al. 2019

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Changes in the kinetics of the creatine kinase (CK) shuttle are sensitive markers of cardiac energetics but are typically measured at rest and in the prone position. This study aims to measure CK kinetics during pharmacological stress at 3 T, with measurement in the supine position. A shorter “stressed saturation transfer” (StreST) extension to the triple repetition time saturation transfer (TRiST) method is proposed. We assess scanning in a supine position and validate the MR measurement against biopsy assay of CK activity. We report normal ranges of stress CK forward rate (k f CK ) for healthy volunteers and obese patients. TRiST measures k f CK in 40 min at 3 T. StreST extends the previously developed TRiST to also make a further k f CK measurement during <20 min of dobutamine stress. We test our TRiST implementation in skeletal muscle and myocardium in both prone and supine positions. We evaluate StreST in the myocardium of six healthy volunteers and 34 obese subjects. We validated MR‐measured k f CK against biopsy assays of CK activity. TRiST k f CK values matched literature values in skeletal muscle (k f CK = 0.25 ± 0.03 s −1 vs 0.27 ± 0.03 s −1 ) and myocardium when measured in the prone position (0.32 ± 0.15 s −1 ), but a significant difference was found for TRiST k f CK measured supine (0.24 ± 0.12 s −1 ). This difference was because of different respiratory‐ and cardiac‐motion‐induced B 0 changes in the two positions. Using supine TRiST, cardiac k f CK values for normal‐weight subjects were 0.15 ± 0.09 s −1 at rest and 0.17 ± 0.15 s −1 during stress. For obese subjects, k f CK was 0.16 ± 0.07 s −1 at rest and 0.17 ± 0.10 s −1 during stress. Rest myocardial k f CK and CK activity from LV biopsies of the same subjects correlated ( R = 0.43, p = 0.03). We present an independent implementation of TRiST on the Siemens platform using a commercially available coil. Our extended StreST protocol enables cardiac k f CK to be measured during dobutamine‐induced stress in the supine position.

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Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T

et al. 2019

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“…This prohibits the spatially localized measurements of CK reaction rate constants in vivo. Several techniques have been developed to optimize the acquisition strategy for speedier CK flux measurements [15][16][17][18][19][20][21]. These techniques rely on pulse sequences that provide little spatial localization [21] or 1D-CSI spatial localization in the myocardium [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Creatine kinase rate constant in the human heart at 7T with 1D-ISIS/2D CSI localization

2020

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PurposeCreatine Kinase (CK) reaction plays an important role in energy metabolism and estimate of its reaction rate constant in heart provides important insight into cardiac energetics. Fast saturation transfer method (T nom 1 À T1 nominal) to measure CK reaction rate constant (k f ) was previously demonstrated in open chest swine hearts. The goal of this work is to further develop this method for measuring the k f in human myocardium at 7T. T nom 1 approach is combined with 1D-ISIS/2D-CSI for in vivo spatial localization and myocardial CK forward rate constant was then measured in 7 volunteers at 7T. MethodsT nom 1 method uses two partially relaxed saturation transfer (ST) spectra and correction factor to determine CK rate constant. Correction factor is determined by numerical simulation of Bloch McConnell equations using known spin and experimental parameters. Optimal parameters and error estimate in calculation of CK reaction rate constant were determined by simulations. The technique was validated in calf muscles by direct comparison with saturation transfer measurements. T nom 1 pulse sequence was incorporated with 1D-image selected in vivo spectroscopy, combined with 2D-chemical shift spectroscopic imaging (1D-ISIS/2D-CSI) for studies in heart. The myocardial CK reaction rate constant was then measured in 7 volunteers. ResultsSkeletal muscle k f determined by conventional approach and T nom 1 approach were the same 0.31 ± 0.02 s -1 and 0.30 ± 0.04 s -1 demonstrating the validity of the technique. Results are reported as mean ± SD. Myocardial CK reaction rate constant was 0.29 ± 0.05 s -1 , consistent with previously reported studies. ConclusionT nom 1 method enables acquisition of 31 P saturation transfer MRS under partially relaxed conditions and enables 2D-CSI of k f in myocardium. This work enables applications for in vivo CSI imaging of energetics in heart and other organs in clinically relevant acquisition time.

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Quantification of creatine kinase reaction rate in mouse hindlimb using phosphorus‐31 magnetic resonance spectroscopic fingerprinting

Kim

Wang

et al. 2020

NMR in Biomedicine

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The goal of this study was to evaluate the accuracy, reproducibility, and efficiency of a 31 P magnetic resonance spectroscopic fingerprinting (31 P-MRSF) method for fast quantification of the forward rate constant of creatine kinase (CK) in mouse hindlimb. The 31 P-MRSF method acquired spectroscopic fingerprints using interleaved acquisition of phosphocreatine (PCr) and γATP with ramped flip angles and a saturation scheme sensitive to chemical exchange between PCr and γATP. Parameter estimation was performed by matching the acquired fingerprints to a dictionary of simulated fingerprints generated from the Bloch-McConnell model. The accuracy of 31 P-MRSF measurements was compared with the magnetization transfer (MT-MRS) method in mouse hindlimb at 9.4 T (n = 8). The reproducibility of 31 P-MRSF was also assessed by repeated measurements. Estimation of the CK rate constant using 31 P-MRSF (0.39 ± 0.03 s −1) showed a strong agreement with that using MT-MRS measurements (0.40 ± 0.05 s −1). Variations less than 10% were achieved with 2 min acquisition of 31 P-MRSF data. Application of the 31 P-MRSF method to mice subjected to an electrical stimulation protocol detected an increase in CK rate constant in response to stimulation-induced muscle contraction. These results demonstrated the potential of the 31 P-MRSF framework for rapid, accurate, and reproducible quantification of the chemical exchange rate of CK in vivo.

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Creatine kinase rate constant in the human heart measured with 3D‐localization at 7 tesla

Cited by 17 publications

References 43 publications

Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T

Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T

Creatine kinase rate constant in the human heart at 7T with 1D-ISIS/2D CSI localization

Quantification of creatine kinase reaction rate in mouse hindlimb using phosphorus‐31 magnetic resonance spectroscopic fingerprinting

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