Creatine kinase isozyme expression in embryonic chicken heart

Abstract

The distribution pattern of creatine kinase (EC 2.7.3.2) isozymes in developing chicken heart was studied by immunohistochemistry. Creatine kinase M, which is absent from adult heart, is transiently expressed between 4 and 11 days of incubation. During that period, numerous muscular cells in the roof and septum of the atrium, in the interventricular septum and on top of the trabeculae cordis and at the rim of the outflow tract stain strongly with a polyclonal antibody that is specific for the M subunit. In the ventricle and outflow tract, the M-positive cells are found mainly subendocardially and in the right half, at the transition of conducting and working myocytes. Creatine kinase B, which is the predominant adult isozyme, is initially expressed to a high concentration in a small group of disperse myocardial cells in upstream part of the inflow tract. When compared to the expression pattern of cardiac myosin heavy chains, the observed creatine kinase expression pattern suggests that M-positive cells are mainly found in areas that participate in the formation of cardiac conductive tissue, whereas B-positive cells are first found in areas that are involved in the generation of cardiac rhythm.

Rat β3-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues

“…Such differentiation can also be derived from the indirect comparison in our immunoblotting and the immunocytochemical experiments by (Kullmann et al 2009) using AB15688. Apparently, more epitopes are exposed after extensive denaturation as done in immunoblots than in immunocytochemical or immunohistochemical studies, as previously also demonstrated for antibodies against creatine kinase (Lamers et al 1989). Studies on glutamic acid decarboxylase also support the notion that linear structures expose different epitopes for antibody recognition than those found in complex threedimensional conformations (Al-Bukhari et al 2002).…”

Creatine kinase isozyme expression in prenatal rat heart

“…In particular, CK-M expression may be an early enzymatic parameter of the developmental appearance of the ventricular conductive tissue. In all other species investigated (Forsgren et al 1983;Lamers et al 1989;Wessels et al 1990a), CK-M expression is initially found in the subendocardial layer of the ventricular walls and in the trabeculae. Subsequently, a high concentration of CK-M accumulates in the bundle branches, i.e.…”

“…Subsequently, a high concentration of CK-M accumulates in the bundle branches, i.e. the presumably rapidly conducting structures of human, bovine and chicken embryos (Forsgren et al 1983;Lamers et al 1989;Wessels etal. 1990a), whereas the atrioventricular junction, atrioventricular node and the proximal part of the bundle of His, i.e.…”

Development of the Cardiac Conduction System

“…181 Creatine kinase-M is virtual absent from the flanking segments (IFT, AVC, and OFT) and was much higher in the TVC than the CVC. Higher creatine kinase-M levels are also found to be associated with the developing ventricular conduction system in chicken, 182 rat, 183 and bovine 184 hearts.…”