Creatine Kinase

Bais, Renze; Edwards, J.B.

doi:10.3109/10408368209107030

Cited by 62 publications

(34 citation statements)

References 296 publications

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“…Therefore, it can be said that LIH index of Olympus can be used to identify the inappropriate hemolyzed samples but this is not reliable enough for CK-MB activity in the samples with normal range. It has been previously documented that adenylate kinase catalyzes the reaction; 2 ADP ßà AMP + ATP, and increased AK levels in hemolyzed samples leads to an apparent increase in creatine kinase activity [14,15]. It can be diminished by the addition of either AMP, which is a weak competitive inhibitor of AK, or diadenosine pentaphosphate, which is a powerful competitive inhibitor (2).…”

Section: Discussionmentioning

confidence: 99%

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Özcan¹,

Karakas²,

Yücel³

2012

Turk J Bioch

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Aim: The aim of the study was to investigate the effects of in vitro hemolysis on serum creatine kinase and creatine kinase MB isoenzyme activities with creatine kinase MB mass, troponin I and myoglobin measurements. Materials and methods:We prepared serum pools having analyte concentrations at normal and pathological values. Hemolysate was added into serum pools to obtain aliquotes with final hemoglobin concentrations of 21, 10.5, 5.25, 2.625, 1.312, 0.656, 0.328, 0.164, 0.08 and 0.041 g/L. Creatine kinase (by N-acetylcysteine-activated IFCC method) and creatine kinase MB activities (by immunoinhibition method) were measured in these pools. Creatine kinase MB (mass), troponin I and myoglobin concentrations were measured by chemiluminescence method. Mean percent changes were calculated and graphed as interferographs. Results: It was found that the positive interference due to hemolysis began to exceed the limit of 10% at lower Hb concentration for CK-MB activity than CK activity. When reference change value were considered, the critical effect of hemolysis began at higher hemoglobin concentrations. Hemolysis did not affect creatine kinase MB mass, troponin and myoglobin measurements when the limit of 10% change was considered. Conclusions: CK-MB activity is affected more profoundly than CK activity by hemolysis.

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Section: Discussionmentioning

confidence: 99%

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Özcan¹,

Karakas²,

Yücel³

2012

Turk J Bioch

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“…Normal human serum contains MM-CK almost exclusively and it has been suggested that serum BB-CK could be used as a tumour marker (Foreback and Chu, 1981;Bais and Edwards, 1982;Grifflths, 1982;Nanji, 1983;Kanemitsu and Okigaki, 1988). Serum BB-CK increases in patients with small-cell lung carcinoma, which has higher BB-CK levels than the normal lung tissue.…”

Section: Distribution Of Ck Isoenzymes In Liver Normal Tissue and Tummentioning

confidence: 99%

“…Many reports have been published on the distribution of CK subunits and isoenzymes in tumours (for review see Foreback and Chu, 1981;Bais and Edwards, 1982;Griffiths, 1982;Nanji, 1983;Kanemitsu and Okigaki, 1988). However, most data have been obtained by immunohistochemical and immunoassay techniques.…”

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confidence: 99%

Creatine kinase activity and isoenzymes in lung, colon and liver carcinomas

Joseph

Cardesa²,

Carreras³

1997

Br J Cancer

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SummaryWe have compared the levels of creatine kinase (CK) activity and the distribution of CK isoenzymes determined by agarose gel electrophoresis in normal colon, liver and lung tissues, and in colon, liver and lung adenocarcinomas, lung squamous cell carcinomas and lung carcinoids. Colon and lung adenocarcinomas, and squamous cell carcinomas presented lower CK activity than the normal tissues and no differences were found between hepatocarcinoma and normal liver tissue. In contrast, lung carcinoids had higher CK activity than normal lung tissue. Type BB-CK was the predominant isoenzyme in normal lung, colon and liver tissues. Type MM isoenzyme was detected in normal lung and type MB-CK was found in normal colon. In most lung tumours the CK isoenzyme electrophoretic pattern did not change. However, no type BB-CK was detected in some hepatocarcinomas, type MM-CK decreased in lung carcinoids and type MB isoenzyme was not observed in colon adenocarcinomas. It is concluded that in most tumours there is a decrease in the expression of type B-and type M-CK subunits, whereas in lung carcinoid the expression of type B-CK activity increases. Thus, the increase in type BB-CK observed in the serum of patients with lung and colon adenocarcinomas is probably due mainly to enhanced enzyme release as a result of tumour cell necrosis.

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“…Application of the lower creatine kinase-MB fraction limit, with a view to eliminating false positive results arising from either incomplete inhibition of creatine kinase-MM activity (5) or creatine kinase-MB activity from skeletal muscle damage (1,4), has been discussed by others (10,23). The disadvantage is that an acute myocardial infarction could be masked by a simultaneous major release of creatine kinase-MM from non-myocardial sources.…”

Section: Discussionmentioning

confidence: 99%

“…The aim of this specific diagnostic test. The most widely used single study is to compare two different immunoinhibition laboratory test is electrophoretic determination of the methods (a dry and a wet chemistry method) with the MB isoenzyme of creatine kinase (EC 2.7.3.2) 1 ) (crea-commonly used but laborious electrophoretic method, tine kinase-MB) (1,2). This method is very specific and reasonably sensitive, but it is rather tedious and expensive (3,4).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

Hadberg¹,

Hassager²,

Hildebrandt³

et al. 1990

Clinical Chemistry and Laboratory Medicine

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Summary:Two immunoinhibitory methods for measuring creatine kinase-MB (a dry chemical and a wet chemical method) were compared with the commonly used agarose gel electrophoretic method, using 563 serum samples from 235 patients with suspected acute myocardial infarction. Comparison of the electrophoretic and the dry chemistry methods showed the linear relationship: electrophoretic method = -6.5 U/l + 1.22 χ dry chemistry method, r = 0.943. For the wet chemistry method the relationship was: electrophoretic method = -7.2 U/l + 1.19 x wet chemistry method, r = 0.854. Parallel determinations of total creatine kinase were also done and the methods were virtually identical in performance.Compared with the electrophoretic method (which showed a 15% prevalence of acute myocardial infarction), these classifications showed sensitivities of 0.92 and 0.67 and specificities of 0.94 and 0.99 (dry and wet chemistry, respectively; using methods recommended by the manufacturers). After optimization of discriminators the sensitivity was increased to 0.94/0.92 and the specificity to 0.99/0.99. Introduction." ., ,. . , specific antibodies against the subumt M. The antiAcute myocardial infarction is one of the commonest bodies have either been used in radioimmunoassays causes of hospitalization and death in the western or for specific inhibition of the enzymatic activity of industrialized countries. It is therefore of great im-the subunit M, followed by measurement of the reportance to have a fast, inexpensive, sensitive, and maining enzymatic activity (2, 5). The aim of this specific diagnostic test. The most widely used single study is to compare two different immunoinhibition laboratory test is electrophoretic determination of the methods (a dry and a wet chemistry method) with the MB isoenzyme of creatine kinase (EC 2.7.3.2) 1 ) (crea-commonly used but laborious electrophoretic method, tine kinase-MB) (1, 2). This method is very specific and reasonably sensitive, but it is rather tedious and expensive (3, 4). The last decade has seen the devel-Materials and Methods opment of easier analytical methods, which utilize Samples

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Creatine Kinase

Cited by 62 publications

References 296 publications

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Effects of hemolysis on the assays of serum CK, CK-MB activities and CK-MB (MASS), troponin and myoglobin measurments

Creatine kinase activity and isoenzymes in lung, colon and liver carcinomas

Comparison of Two Immunoinhibitory Methods with Agarose Gel-Electrophoresis for Measuring the MB Isoenzyme of Creatine Kinase in Serum from Cases of Suspected Myocardial Infarction

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