Crataegus pinnatifida Bunge Inhibits RANKL-Induced Osteoclast Differentiation in RAW 264.7 Cells and Prevents Bone Loss in an Ovariectomized Rat Model

Kim, Min-Sun; Kim, Min‐Beom; Kim, Jae Hyun; Hong, Sooyeon; Kim, Dong Hee; Kim, Sang Woo; Kim, Eun Young; Jung, Hyuk‐Sang; Sohn, Youngjoo

doi:10.1155/2021/5521562

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…c-Fos also controls expression of CA2, which serves an important role in osteoclast bone absorption function ( 47 ). CA2 acidifies the bone surface prior to osteoclast-mediated bone absorption, creating an environment in which various enzymes can function ( 48 , 49 ). In the present study, SL inhibited both c-Fos and CA2 expression.…”

Section: Discussionmentioning

confidence: 99%

Solanum nigrum Line inhibits osteoclast differentiation and suppresses bone mineral density reduction in the ovariectomy‑induced osteoporosis model

et al. 2021

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Bone homeostasis is maintained by osteoclasts that absorb bone and osteoblasts that form bone tissue. Menopausal osteoporosis is a disease associated with aging and hormonal changes due to menopause causing abnormal activation of osteoclasts, resulting in a decrease in bone density. existing treatments for osteoporosis have been reported to have serious side effects, such as jawbone necrosis and breast and uterine cancer; therefore, their use by patients is decreasing, whilst studies focusing on alternative treatments are increasingly popular. Solanum nigrum line (Sl) has been used as a medicinal plant that possesses several pharmacological effects, such as anti-inflammatory and hepatotoxic protective effects. To the best of our knowledge, however, its effects on osteoporosis and osteoclasts have not been demonstrated previously. in the present study, the anti-osteoporotic effect of Sl was investigated using a postmenopausal model of osteoporosis in which Sprague-dawley rat ovaries were extracted. in addition, the inhibitory effects on osteoclast differentiation and function of SL was confirmed using an osteoclast model treated with receptor activator of nF-κB ligand (ranKl) on murine raW 264.7 macrophages. In vivo experiments showed that Sl reduced the decrease in bone mineral density and improved changes in the morphological index of bone microstructure, such as trabecular number and separation. in addition, the number of tartrate resistant acid phosphatase-positive cells in the femur and the expression levels of nuclear factor of activated T-cells cytoplasmic 1 (nFaTc1) and cathepsin K protein were inhibited. In vitro, Sl suppressed ranKl-induced osteoclast differentiation and bone resorption ability; this was mediated by nFaTc1/c-Fos, a key transcription factor involved in osteoclast differentiation, ultimately inhibiting expression of various osteoclast-associated genes. These experimental results show that Sl may be an alternative treatment for osteoporosis caused by abnormal activation of osteoclasts in the future.

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Section: Discussionmentioning

confidence: 99%

Solanum nigrum Line inhibits osteoclast differentiation and suppresses bone mineral density reduction in the ovariectomy‑induced osteoporosis model

et al. 2021

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“…The whole hawthorn extract and its components exhibited other bioactivities in vitro. The whole hawthorn extract inhibited osteoclast differentiation by suppressing the nuclear factor of activated T cells (NFATc1) and c‐Fos expression and suppressing the expression of osteoclast‐related genes, such as NFATc1, Ca2, Acp5, mmp9, cathepsin K (CtsK), Oscar, and Atp6v0d2 (Kim et al., 2021). Proanthocyanidins and phenylpropanoids extracted from the whole hawthorn fruit exhibited moderate tyrosinase inhibitory activity (Liang et al., 2022) (Table 1).…”

Section: Bioactivities Based On In Vitro and In Vivo Studiesmentioning

confidence: 99%

The hawthorn (Crataegus pinnatifida Bge.) fruit as a new dietary source of bioactive ingredients with multiple beneficial functions

Ma,

Yang,

et al. 2024

Food Frontiers

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The discovery of new natural sources has brought increased attention to the development of functional foods. The hawthorn (Crataegus pinnatifida Bge.) fruit is an underutilized fruit due to its benefits for human health and good taste. It contains a variety of bioactive ingredients, contributing to its multiple beneficial functions and applications. This review summarized and discussed the main bioactive ingredients, beneficial functions based on in vitro, in vivo, and human studies, and different applications of the hawthorn fruit according to the updated literature in the past 3 years. Hawthorn berries contain phenolic acids, flavonoids, proanthocyanidins, pectin, and many other bioactive components, which have a variety of beneficial functions, such as antioxidant, anti‐inflammatory, antibacterial, antidiabetic, intestinal protection, cardiovascular protection, hepatoprotection, anti‐cancer, and neuroprotection. Its potential molecular mechanism and different food‐related applications such as hawthorn wine and antioxidant drink are discussed in detail in this review. Additionally, hawthorn berries are shown to be safe when consumed within the proper dosage. Collectively, this updated review indicates that the hawthorn fruit can be a new dietary source of bioactive ingredients with multiple beneficial functions and can be affordably developed into functional and medicinal foods for the prevention and management of certain chronic diseases.

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“…Based on these findings, rats in the MFR-H groups were treated with MFR at concentrations 10-fold higher than those in the MFR-L group to induce higher pharmacological effects. Therefore, the MFR-H group was treated with 169 mg/kg MFR (33)(34)(35). Oral administration was performed every morning for 8 weeks.…”

Section: Semi-quantitative Reverse Transcription-pcr (Rt-pcr)mentioning

confidence: 99%

Effects ofMelandrium firmumRohrbach on RANKL‑induced osteoclast differentiation and OVX rats

Kim

Hong

et al. 2021

Mol Med Rep

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Osteoporosis is a systemic skeletal disease characterized by reduced bone mineral density (BMD), which results in an increased risk of fracture. Melandrium firmum (Siebold & Zucc.) Rohrbach (MFR), 'Wangbulryuhaeng' in Korean, is the dried aerial portion of Melandrii Herba Rohrbach, which is a member of the Caryophyllaceae family and has been used to treat several gynecological conditions as a traditional medicine. However, to the best of our knowledge, the effect of MFR on osteoclast differentiation and osteoporosis has not been assessed. To evaluate the effects of MFR on osteoclast differentiation, tartrate-resistant acid phosphatase staining, actin ring formation and bone resorption assays were used. Additionally, receptor activator of nuclear factor-κB ligand-induced expression of nuclear factor of activated T cell, cytoplasmic 1 (NFATc1) and c-Fos were measured using western blotting and reverse transcription-PCR. The expression levels of osteoclast-related genes were also examined. To further investigate the anti-osteoporotic effects of MFR in vivo, an ovariectomized (OVX) rat model of menopausal osteoporosis was established. Subsequently, the femoral head was scanned using micro-computed tomography. The results revealed that MFR suppressed osteoclast differentiation, formation and function. Specifically, MFR reduced the expression levels of osteoclast-related genes by downregulating transcription factors, such as NFATc1 and c-Fos. Consistent with the in vitro results, administration of MFR water extract to OVX rats reduced BMD loss, and reduced the expression levels of NFATc1 and cathepsin K in the femoral head. In conclusion, MFR may contribute to alleviate osteoporosis-like symptoms. These results suggested that MFR may exhibit potential for the prevention and treatment of postmenopausal osteoporosis.

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Crataegus pinnatifida Bunge Inhibits RANKL-Induced Osteoclast Differentiation in RAW 264.7 Cells and Prevents Bone Loss in an Ovariectomized Rat Model

Cited by 4 publications

References 53 publications

Solanum nigrum Line inhibits osteoclast differentiation and suppresses bone mineral density reduction in the ovariectomy‑induced osteoporosis model

Solanum nigrum Line inhibits osteoclast differentiation and suppresses bone mineral density reduction in the ovariectomy‑induced osteoporosis model

The hawthorn (Crataegus pinnatifida Bge.) fruit as a new dietary source of bioactive ingredients with multiple beneficial functions

Effects ofMelandrium firmumRohrbach on RANKL‑induced osteoclast differentiation and OVX rats

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