Crataegus monogyna buds and fruits phenolic extracts: Growth inhibitory activity on human tumor cell lines and chemical characterization by HPLC–DAD–ESI/MS

Abstract

Crataegus monogyna has been extensively studied due to its various alleged health benefits. This study aimed to determine the human tumour cells growth inhibitory activity of phenolic extracts of its flower buds and fruits in three phenological stages, and further characterize the extracts by HPLC-DAD-ESI/MS. Flower bud extract showed the highest antiproliferative activity as indicated by the lowest GI 50 values obtained in all the tested cell lines: MCF-7, breast adenocarcinoma; NCI-H460, nonsmall cell lung cancer; HeLa, cervical carcinoma; HepG2, hepatocellular carcinoma.Furthermore, porcine liver primary cell culture (PLP2) was used to evaluate toxicity to non-tumour cells. Flavonoids, particularly flavonols and flavones (higher in flower buds) and proanthocyanidins (higher in unripe fruits) were the main classes in the studied samples. Phenolic acids (mainly hydroxycinnamic acid derivatives) were also detected in significant amounts, especially in flower bud extract. Regarding anthocyanins, over ripened fruits gave the highest content. The higher bioactivity observed in flower buds might be related with its higher content in phenolic compounds.

Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents

“…A porcine liver primary cells culture, established in our laboratory (PLP2), was used to evaluate the hepatotoxicity of the extracts, following a previously described methodology (Rodrigues et al, 2012). The results were expressed in GI 50 values, i.e.…”

Nutritional composition, antioxidant activity and phenolic compounds of wild Taraxacum sect. Ruderalia

“…Phenolic compounds were determined by HPLC (Hewlett-Packard 1100, Agilent Technologies, Santa Clara, USA) (Rodrigues et al, 2012). Double online detection was carried out in the diode array detector (DAD) using 280 nm and 370 nm as preferred wavelengths and in a mass spectrometer (API 3200 Qtrap, Applied Biosystems, Darmstadt, Germany) connected to the HPLC system via the DAD cell outlet.…”

Total phenolic, flavonoid and antioxidant activity of 23 edible flowers subjected to in vitro digestion

“…Phenolics have strong in vitro and in vivo antioxidant activities, which are associated with their ability to scavenge free radicals, break radical chain reactions, and chelate metals (Chiang, Kadouh, & Zhou, 2013;Niki, 2010;Qingming et al, 2010). Increased consumption of phenolics has been correlated with anti-inflammatory activity and a reduced risk of cardiovascular disease and certain cancers (Hamaguchi, Ono, Murase, & Yamada, 2009;Kaz Owska, Hsu, Hou, Yang, & Tsai, 2010;Oueslati et al, 2012;Rodrigues et al, 2012). Total phenolics and the antioxidant capacities of ten flowers were measured in China; the results indicated that flowers were good sources of phenolics and antioxidants (Xiong et al, 2014).…”