Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland

Romero-Geraldo, Reyna; García-Lagunas, Norma; Hernández-Saavedra, Norma Y.

doi:10.1016/j.marenvres.2016.07.011

Cited by 25 publications

(16 citation statements)

References 57 publications

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“…The analysis of our results showed that the expression level in the studied genes was affected by cell concentration of the toxic dinoflagellates, as well as exposure time on C. gigas spat. These observations were consistent with those reported by other authors Romero-Geraldo & Hernández-Saavedra, 2014;García-Lagunas et al, 2013Romero-Geraldo et al, 2014, 2016. The expression level of cvt and lgbp gene showed a similar tendency, up-regulated for the three treatments at day three and down-regulated until the end of the experiment, indicating that DSP and PSP toxins inhibited the expression of these genes after five days of feeding.…”

supporting

confidence: 92%

“…Paralytic shellfish toxins (PSTs) are a group of neurotoxic alkaloids, grouped into three structural families: carbamate (saxitoxin-STX, neosaxitoxin-NEO and gonyautoxins-GTX1 to GTX4), decarbamoyl and N-sulfocarbamoyl (Oshima, 1995). These dinoflagellate species have been associated with DSP and PSP episodes in different parts of the world, and in the Gulf of California, they have also been extensively used in aquatic toxicological studies (Pinto-Silva et al, 2005;Wikfors, 2005;Flórez-Barrós et al, 2011;García-Lagunas et al, 2013Romero-Geraldo et al, 2014, 2016.…”

mentioning

confidence: 99%

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Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

Kao-Godinez¹,

Romero-Geraldo²,

García-Lagunas³

et al. 2019

LAJAR

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The effects of Gymnodynium catenatum and Prorocentrum lima, dinoflagellate species present in the Gulf of California, on marine bivalves, have demonstrated physiological alterations and changes in gene expression patterns; however, modification effects of the genes involved in immune defense are still poorly understood. This study analyzed the mRNA levels of five genes encoding for the immune defense of oyster Crassostrea gigas spat fed with a control diet (Isochrysis galbana alone) and a combination of the two toxic dinoflagellates. Expression levels of lipopolysaccharide (LPS)-binding protein 1,3-glucan and cavorting genes were higher in oysters exposed to the combined diets in all treatments compared to the non-toxic diet at day three, which was probably related with an activation of the oysters' immediate immune response during the first 24 h. Protein 44 interferon-induced gene expression level was repressed in treatments with the highest dinoflagellate concentration and overexpressed in the diet with equal dinoflagellate concentration. Interaction protein-Toll and immunoglobulin gene transcript levels reached the highest values at day seven in oysters exposed to all combined diets. Then, the immune defense appeared to be activated in oyster spat as a response of toxins and/or extra-cellular compounds produced by the dinoflagellates.

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supporting

confidence: 92%

mentioning

confidence: 99%

Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

Kao-Godinez¹,

Romero-Geraldo²,

García-Lagunas³

et al. 2019

LAJAR

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“…Indeed, CPLA2 is used as a stress indicator in biomonitoring programs. Some authors have also suggested that the up-regulation of genes involved in the inflammatory process, which was observed when digestive glands of the oyster C. gigas were exposed to P. lima , might represent a risk to this bivalve’s integrity [ 55 ]. Heavy metals functionally alter lysosomal membranes in haemocytes of mussels [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis for Assessing the Early Response to DSP Toxins in Mytilus galloprovincialis Digestive Gland and Gill

Prego-Faraldo

Martínez

Méndez

2018

Toxins

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The harmful effects of diarrhetic shellfish poisoning (DSP) toxins on mammalian cell lines have been widely assessed. Studies in bivalves suggest that mussels display a resistance to the cytogenotoxic effects of DSP toxins. Further, it seems that the bigger the exposure, the more resistant mussels become. To elucidate the early genetic response of mussels against these toxins, the digestive gland and the gill transcriptomes of Mytilus galloprovincialis after Prorocentrum lima exposure (100,000 cells/L, 48 h) were de novo assembled based on the sequencing of 8 cDNA libraries obtained using an Illumina HiSeq 2000 platform. The assembly provided 95,702 contigs. A total of 2286 and 4523 differentially expressed transcripts were obtained in the digestive gland and the gill, respectively, indicating tissue-specific transcriptome responses. These transcripts were annotated and functionally enriched, showing 44 and 60 significant Pfam families in the digestive gland and the gill, respectively. Quantitative PCR (qPCR) was performed to validate the differential expression patterns of several genes related to lipid and carbohydrate metabolism, energy production, genome integrity and defense, suggesting their participation in the protective mechanism. This work provides knowledge of the early response against DSP toxins in the mussel M. galloprovincialis and useful information for further research on the molecular mechanisms of the bivalve resistance to these toxins.

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“…The frequent appearance of toxin-producing harmful algal blooms (HABs) in marine environments is a well-known worldwide problem [ 5 ]. HABs are well known for their potential to induce ecological damage, risk human health, and cause adverse effects to living marine resources [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid

Cheng

Giri

Jun

et al. 2018

Toxins

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To reveal the molecular mechanisms triggered by okadaic acid (OA)-exposure in the detoxification and immune system of bay scallops, we studied differentially-expressed genes (DEGs) and the transcriptomic profile in bay scallop gill tissue after 48 h exposure to 500 nM of OA using the Illumina HiSeq 4000 deep-sequencing platform. De novo assembly of paired-end reads yielded 55,876 unigenes, of which 3204 and 2620 genes were found to be significantly up- or down-regulated, respectively. Gene ontology classification and enrichment analysis of the DEGs detected in bay scallops exposed to OA revealed four ontologies with particularly high functional enrichment, which were ‘cellular process’ (cellular component), ‘metabolic process’ (biological process), ‘immune system process’ (biological process), and ‘catalytic process’ (molecular function). The DEGs revealed that cyclic AMP-responsive element-binding proteins, acid phosphatase, toll-like receptors, nuclear erythroid 2-related factor, and the NADPH2 quinone reductase-related gene were upregulated. In contrast, the expression of some genes related to glutathione S-transferase 1, C-type lectin, complement C1q tumor necrosis factor-related protein, Superoxide dismutase 2 and fibrinogen C domain-containing protein, decreased. The outcomes of this study will be a valuable resource for the study of gene expression induced by marine toxins, and will help understanding of the molecular mechanisms underlying the scallops’ response to OA exposure.

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Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland

Cited by 25 publications

References 57 publications

Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

Differential expression of immune response genes in Pacific oyster, Crassostrea gigas spat, fed with dinoflagellates Gymnodinium catenatum and Prorocentrum lima

RNA-Seq Analysis for Assessing the Early Response to DSP Toxins in Mytilus galloprovincialis Digestive Gland and Gill

Detoxification- and Immune-Related Transcriptomic Analysis of Gills from Bay Scallops (Argopecten irradians) in Response to Algal Toxin Okadaic Acid

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