Craniofacial Cartilage Tissue Engineering

Watson, Deborah; Watson, Jeffrey B.; Sah, Robert L.; Briggs, Kristen K.

doi:10.1016/b978-0-12-397157-9.00045-x

Cited by 1 publication

(2 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data shown in Figure D demonstrate the initial degradation of the submerged material as it absorbs the surrounding phosphate-buffered saline (PBS) but becomes stable around day 14 (336 h). This suggests a weak but effective cross-linking network, which could potentially allow for the gas and nutrient exchanges that are essential for cell growth and survival …”

Section: Discussionmentioning

confidence: 99%

“…This suggests a weak but effective cross-linking network, which could potentially allow for the gas and nutrient exchanges that are essential for cell growth and survival. 38 For cells to survive within a 3D culture, the platform must be porous enough to allow gas and nutrient exchange. In the current study, PCs were acquired from healthy septal cartilage.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bioprinting of Chondrocyte Stem Cell Co-Cultures for Auricular Cartilage Regeneration

et al. 2022

View full text Add to dashboard Cite

Advances in 3D bioprinting allows not only controlled deposition of cells or cell-laden hydrogels but also flexibility in creating constructs that match the anatomical features of the patient. This is especially the case for reconstructing the pinna (ear), which is a large feature of the face and made from elastic cartilage that primarily relies on diffusion for nutrient transfer. The selection of cell lines for reconstructing this cartilage becomes a crucial step in clinical translation. Chondrocytes and mesenchymal stem cells are both studied extensively in the area of cartilage regeneration as they are capable of producing cartilage in vitro. However, such monoculture systems involve unfavorable processes and produce cartilage with suboptimal characteristics. Co-cultures of these cell types are known to alleviate these limitations to produce synergically active chondrocytes and cartilage. The current study utilized a 3D bioprinted scaffold made from combined gelatine methacryloyl and methacrylated hyaluronic acid (GelMA/HAMA) to interrogate monocultures and co-cultures of human septal chondrocytes (primary chondrocytes, PCs) and human bone marrow-derived mesenchymal stem cells (BM-hMSCs). This study is also the first to examine co-cultures of healthy human chondrocytes with human BM-hMSCs encapsulated in GelMA/HAMA bioprinted scaffolds. Findings revealed that the combination of MSCs and PCs not only yielded cell proliferation that mimicked MSCs but also produced chondrogenic expressions that mimicked PCs. These findings suggested that co-cultures of BM-hMSCs and healthy septal PCs can be employed to replace monocultures in chondrogenic studies for cartilage regeneration in this model. The opportunity for MSCs used to replace PCs alleviates the requirement of large cartilage biopsies that would otherwise be needed for sufficient cell numbers and therefore can be employed for clinical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%