Cranial bone structure in children with sagittal craniosynostosis: Relationship with surgical outcomes

Rodríguez-Flórez, Naiara; Ibrahim, Amel; Hutchinson, J. Ciaran; Borghi, Alessandro; James, Greg; Arthurs, Owen J.; Ferretti, Patrizia; Dunaway, David; Schievano, Silvia; Jeelani, Owase

doi:10.1016/j.bjps.2017.06.017

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…Quantification of bone volume (mineralized matrix) and total volume (mineral and organic components) was carried out and bone fraction volume (BV/TV), a measure of mineralized bone density, calculated using BoneJ 28 . The average BV/TV was 0.46 in the data set of calvarial bone from children aged 3.7 to 7.4 months old in line with previous studies by our group 29 …”

Section: Resultssupporting

confidence: 77%

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Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Ibrahim

Rodríguez-Flórez

Gardner

et al. 2020

Stem Cells Translational Medicine

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While human adipose-derived stem cells (hADSCs) are known to possess osteogenic differentiation potential, the bone tissues formed are generally considered rudimentary and immature compared with those made by bone-derived precursor cells such as human bone marrow-derived mesenchymal stem cells (hBMSCs) and less commonly studied human calvarium osteoprogenitor cells (hOPs). Traditional differentiation protocols have tended to focus on osteoinduction of hADSCs through the addition of osteogenic differentiation media or use of stimulatory bioactive scaffolds which have not resulted in mature bone formation. Here, we tested the hypothesis that by reproducing the physical as well as biochemical bone microenvironment through the use of three-dimensional (3D) culture and vascularization we could enhance osteogenic maturation in hADSCs. In addition to biomolecular characterization, we performed structural analysis through extracellular collagen alignment and mineral density in our bone tissue engineered samples to evaluate osteogenic maturation. We further compared bone formed by hADSCs, hBMSCs, and hOPs against mature human pediatric calvarial bone, yet not extensively investigated. Although bone generated by all three cell types was still less mature than native pediatric bone, a fibrin-based 3D microenvironment together with vascularization boosted osteogenic maturation of hADSC making it similar to that of bone-derived osteoprogenitors. This demonstrates the important role of vascularization and 3D culture in driving osteogenic maturation of cells easily available but constitutively less committed to this lineage and suggests a crucial avenue for recreating the bone microenvironment for tissue engineering of mature craniofacial bone tissues from pediatric hADSCs, as well as hBMSCs and hOPs.

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Section: Resultssupporting

confidence: 77%

“…28 The average BV/TV was 0.46 in the data set of calvarial bone from children aged 3.7 to 7.4 months old in line with previous studies by our group. 29…”

Section: Structural and Biomolecular Characterization Of Pediatric mentioning

confidence: 99%

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Ibrahim

Rodríguez-Flórez

Gardner

et al. 2020

Stem Cells Translational Medicine

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“…of the skull [ 17 ]. According to previous studies, skull density, acoustic speed, and absorption coefficient trended toward a reduction with increasing age [ 29 , 30 ]. According to the suggestion in the literature, the density of the skull (

) [ 19 ], the acoustic speed (

) [ 18 ], and the absorption coefficient (

) [ 31 ] are also considered in the present study [ 32 , 33 ].…”

Section: Methodsmentioning

confidence: 93%

The Effects of the Structural and Acoustic Parameters of the Skull Model on Transcranial Focused Ultrasound

Zhang

et al. 2021

Sensors

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Transcranial focused ultrasound (tFUS) has great potential in brain imaging and therapy. However, the structural and acoustic differences of the skull will cause a large number of technical problems in the application of tFUS, such as low focus energy, focal shift, and defocusing. To have a comprehensive understanding of the skull effect on tFUS, this study investigated the effects of the structural parameters (thickness, radius of curvature, and distance from the transducer) and acoustic parameters (density, acoustic speed, and absorption coefficient) of the skull model on tFUS based on acrylic plates and two simulation methods (self-programming and COMSOL). For structural parameters, our research shows that as the three factors increase the unit distance, the attenuation caused from large to small is the thickness (0.357 dB/mm), the distance to transducer (0.048 dB/mm), and the radius of curvature (0.027 dB/mm). For acoustic parameters, the attenuation caused by density (0.024 dB/30 kg/m3) and acoustic speed (0.021 dB/30 m/s) are basically the same. Additionally, as the absorption coefficient increases, the focus acoustic pressure decays exponentially. The thickness of the structural parameters and the absorption coefficient of the acoustic parameters are the most important factors leading to the attenuation of tFUS. The experimental and simulation trends are highly consistent. This work contributes to the comprehensive and quantitative understanding of how the skull influences tFUS, which further enhances the application of tFUS in neuromodulation research and treatment.

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“…This ability to evaluate surgical outcomes may enable development of future tailor made micro-CT image generated 3D printed tools and plates focused on the unique individual requirements. 19 In addition to the virtual modelling of surgical tools, micro-CT scanning also has the potential to test for defects as well as tensile strength of prostheses, 20 and may inform the design of more robust implants. Micro-CT imaging has already long been utilised in industry to assess for material deficiencies in electronic assemblies and aircraft engine turbine blade inspection.…”

Section: Craniofacial Surgical Planningmentioning

confidence: 99%

“…3D printing from an extract femur in a19 week gestation foetus with osteogenesis imperfecta femur Type 2a. (a) Micro-CT imaging of the femur with voxel size of 18 µm.…”

mentioning

confidence: 99%

3D printing from microfocus computed tomography (micro-CT) in human specimens: education and future implications

Shelmerdine

Simcock

Hutchinson

et al. 2018

BJR

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Microfocus CT (micro-CT) is an imaging method that provides three-dimensional digital data sets with comparable resolution to light microscopy. Although it has traditionally been used for non-destructive testing in engineering, aerospace industries and in preclinical animal studies, new applications are rapidly becoming available in the clinical setting including post-mortem fetal imaging and pathological specimen analysis. Printing three-dimensional models from imaging data sets for educational purposes is well established in the medical literature, but typically using low resolution (0.7 mm voxel size) data acquired from CT or MR examinations. With higher resolution imaging (voxel sizes below 1 micron, <0.001 mm) at micro-CT, smaller structures can be better characterised, and data sets post-processed to create accurate anatomical models for review and handling. In this review, we provide examples of how three-dimensional printing of micro-CT imaged specimens can provide insight into craniofacial surgical applications, developmental cardiac anatomy, placental imaging, archaeological remains and high-resolution bone imaging. We conclude with other potential future usages of this emerging technique.

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Cranial bone structure in children with sagittal craniosynostosis: Relationship with surgical outcomes

Abstract: Variation in parietal bone micro-structure was associated with the magnitude of head shape changes induced by spring-assisted cranioplasty. This suggests that bone structure analysis could be a valuable adjunct in designing surgical strategies that yield optimal patient-specific outcomes.

Cited by 17 publications

References 33 publications

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

The Effects of the Structural and Acoustic Parameters of the Skull Model on Transcranial Focused Ultrasound

3D printing from microfocus computed tomography (micro-CT) in human specimens: education and future implications

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