Cranberry Dieback Disorder: A New and Emerging Threat to Cranberry Production in British Columbia

Sabaratnam, S. Vikineswary; DiCarlo, Aldo; Fitzpatrick, Sheila M.; Forge, T.

doi:10.17660/actahortic.2009.810.54

Cited by 20 publications

(2 citation statements)

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“…The strongest growth suppression was noted for the two fungi, Botrytis cinerea strain R1 and Diaporthe vaccinii strain IS7. B. cinerea is a necrotrophic fungus that causes diseases in various plant species, including gray mold in rose plants (Ha et al, 2021) and yellow rot in cranberry fruit (Sabaratnam et al, 2016), while D. vaccinii is known to cause upright dieback of cranberry plant shoots and viscid rot as well as field and storage rot of the berries (Oudemans et al, 1998;Michalecka et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

A biofertilizing fungal endophyte of cranberry plants suppresses the plant pathogen Diaporthe

Thimmappa,

Salhi,

Forget

et al. 2024

Front. Microbiol.

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Fungi colonizing plants are gaining attention because of their ability to promote plant growth and suppress pathogens. While most studies focus on endosymbionts from grasses and legumes, the large and diverse group of ericaceous plants has been much neglected. We recently described one of the very few fungal endophytes promoting the growth of the Ericaceae Vaccinium macrocarpon (American cranberry), notably the Codinaeella isolate EC4. Here, we show that EC4 also suppresses fungal pathogens, which makes it a promising endophyte for sustainable cranberry cultivation. By dual-culture assays on agar plates, we tested the potential growth suppression (or biocontrol) of EC4 on other microbes, notably 12 pathogenic fungi and one oomycete reported to infect not only cranberry but also blueberry, strawberry, tomato plants, rose bushes and olive trees. Under greenhouse conditions, EC4 protects cranberry plantlets infected with one of the most notorious cranberry-plant pathogens, Diaporthe vaccinii, known to cause upright dieback and berry rot. The nuclear genome sequence of EC4 revealed a large arsenal of genes potentially involved in biocontrol. About ∼60 distinct clusters of genes are homologs of secondary metabolite gene clusters, some of which were shown in other fungi to synthesize nonribosomal peptides and polyketides, but in most cases, the exact compounds these clusters may produce are unknown. The EC4 genome also encodes numerous homologs of hydrolytic enzymes known to degrade fungal cell walls. About half of the nearly 250 distinct glucanases and chitinases are likely involved in biocontrol because they are predicted to be secreted outside the cell. Transcriptome analysis shows that the expression of about a quarter of the predicted secondary-metabolite gene clusters and glucan and chitin-degrading genes of EC4 is stimulated when it is co-cultured with D. vaccinii. Some of the differentially expressed EC4 genes are alternatively spliced exclusively in the presence of the pathogen, altering the proteins’ domain content and subcellular localization signal, thus adding a second level of proteome adaptation in response to habitat competition. To our knowledge, this is the first report of Diaporthe-induced alternative splicing of biocontrol genes.

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Section: Resultsmentioning

confidence: 99%

A biofertilizing fungal endophyte of cranberry plants suppresses the plant pathogen Diaporthe

Thimmappa,

Salhi,

Forget

et al. 2024

Front. Microbiol.

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“…can cause fruit spoilage during storage [ 23 , 29 ]. Alternaria and Fusarium can cause fruit, leaf, stem or root diseases in cranberry, not only directly but also as secondary pathogens [ 30 ]. In our study, the fungi Alternaria spp.…”

Section: Discussionmentioning

confidence: 99%

Fungi Present in the Clones and Cultivars of European Cranberry (Vaccinium oxycoccos) Grown in Lithuania

Sinkevičienė,

Sinkevičiūtė,

Česonienė

et al. 2023

Plants

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Fungi are associated with the European cranberry (Vaccinium oxycoccos L.) and play important roles in plant growth and disease control, especially in cranberry yields. This article presents the results of a study which was aimed to investigate the diversity of fungi found on different clones and cultivars of the European cranberry grown in Lithuania, causing twigs, leaf diseases and fruit rots. In this study seventeen clones and five cultivars of V. oxycoccos were selected for investigation. Fungi were isolated via the incubation of twigs, leaves and fruit on a PDA medium and identified according to their cultural and morphological characteristics. Microscopic fungi belonging to 14 genera were isolated from cranberry leaves and twigs, with Physalospora vaccinii, Fusarium spp., Mycosphaerella nigromaculans and Monilinia oxycocci being the most frequently isolated fungi. ‘Vaiva’ and ‘Žuvinta’ cultivars were the most susceptible to pathogenic fungi during the growing season. Among the clones, 95–A–07 was the most susceptible to Phys. vaccinii, 95–A–08 to M. nigromaculans, 99–Ž–05 to Fusarium spp. and 95–A–03 to M. oxycocci. Microscopic fungi belonging to 12 genera were isolated from cranberry berries. The most prevalent pathogenic fungi M. oxycocci were isolated from the berries of the cultivars ‘Vaiva’ and ‘Žuvinta’ and clones 95–A–03 and 96–K–05.

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Colletotrichum acutatum (black spot of strawberry)

2022

CABI Compendium

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Cranberry Dieback Disorder: A New and Emerging Threat to Cranberry Production in British Columbia

Cited by 20 publications

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A biofertilizing fungal endophyte of cranberry plants suppresses the plant pathogen Diaporthe

A biofertilizing fungal endophyte of cranberry plants suppresses the plant pathogen Diaporthe

Fungi Present in the Clones and Cultivars of European Cranberry (Vaccinium oxycoccos) Grown in Lithuania

Colletotrichum acutatum (black spot of strawberry)

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