Crabs Eye (Abrus precatorius) Seed and Its Immunomodulatory and Antitumor Properties

Bhutia, Sujit K.; Maiti, Tapas K.

doi:10.1016/b978-0-12-375688-6.10049-0

Cited by 6 publications

(11 citation statements)

References 16 publications

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“…In this regard, lectins from traditional medicinal plants and plant‐based diets are promising anticancer agents, and are widely used in different regions of the world. Moreover, several clinical and preclinical studies demonstrated lectin as a potential alternative therapy for cancer treatment . We and others showed that lectins, including Abrus agglutinin, Abrus abrin, concanavalin A, soybean lectin (SBL) and peanut lectin induce apoptosis in different types of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…AGG has a specificity towards [gal ( β 1 → 3) gal NAc] and belongs to type II ribosome inactivating protein family (RIP II) with a protein synthesis inhibitory concentration (IC 50 ) of 0.469 µg/mL and a lethal dose (LD 50 ) of 5 mg/kg body weight in mice. The A chain has an rRNA N ‐glycosidase activity and irreversibly inhibits protein synthesis in the eukaryotic ribosome …”

Section: Introductionmentioning

confidence: 99%

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Abrus agglutinin promotes irreparable DNA damage by triggering ROS generation followed by ATM‐p73 mediated apoptosis in oral squamous cell carcinoma

Sinha

Panda

Naik

et al. 2017

Molecular Carcinogenesis

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Oral cancer, a type of head and neck cancer, is ranked as one of the top most malignancies in India. Herein, we evaluated the anticancer efficacy of Abrus agglutinin (AGG), a plant lectin, in oral squamous cell carcinoma. AGG selectively inhibited cell growth, and caused cell cycle arrest and mitochondrial apoptosis through a reactive oxygen species (ROS)-mediated ATM-p73 dependent pathway in FaDu cells. AGG-induced ROS accumulation was identified as the major mechanism regulating apoptosis, DNA damage and DNA-damage response, which were significantly reversed by ROS scavenger N-acetylcysteine (NAC). Moreover, AGG was found to interact with mitochondrial manganese-dependent superoxide dismutase that might inhibit its activity and increase ROS in FaDu cells. In oral cancer p53 is mutated, thus we focused on p73; AGG resulted in p73 upregulation and knock down of p73 caused a decrease in AGG-induced apoptosis. Interestingly, AGG-dependent p73 expression was found to be regulated by ROS, which was reversed by NAC treatment. A reduction in the level of p73 in AGG-treated shATM cells was found to be associated with a decreased apoptosis. Moreover, administration of AGG (50 μg/kg body weight) significantly inhibited the growth of FaDu xenografts in athymic nude mice. In immunohistochemical analysis, the xenografts from AGG-treated mice displayed a decrease in PCNA expression and an increase in caspase-3 activation as compared to the controls. In conclusion, we established a connection among ROS, ATM and p73 in AGG-induced apoptosis, which might be useful in enhancing the therapeutic targeting of p53 deficient oral squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Abrus agglutinin promotes irreparable DNA damage by triggering ROS generation followed by ATM‐p73 mediated apoptosis in oral squamous cell carcinoma

Sinha

Panda

Naik

et al. 2017

Molecular Carcinogenesis

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“…For instance, mistletoe lectins, belonging to the RIP II family, inhibit lymphoma growth in mice and are used as an adjuvant therapy to treat various forms of human cancer . In addition, several legume lectins have also been reported to have noteworthy antitumor activity . In this study, we demonstrate that AGG promotes in vitro apoptosis and anti‐angiogenic effects resulting in a potent antitumor response in human breast cancer cells.…”

Section: Discussionmentioning

confidence: 55%

“…Abrus agglutinin (AGG) isolated from the seeds of Abrus precatorius, an herbal medicinal plant in India, is a hetero‐dimeric glycoprotein of 134‐kDa molecular weight. It is composed of two A and two B chains linked through disulfide bridges . This lectin has specificity towards [gal ( β 1 → 3) gal NAc] and belongs to type II ribosome inactivating protein family (RIP II) with a protein synthesis inhibitory concentration (IC 50 ) of 0.469 μg/ml and a lethal dose (LD 50 ) of 5‐mg/kg body weight in mice.…”

mentioning

confidence: 99%

Abrus agglutinin is a potent anti‐proliferative and anti‐angiogenic agent in human breast cancer

Bhutia

Behera

Das

et al. 2016

Intl Journal of Cancer

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Abrus agglutinin (AGG), a plant lectin isolated from the seeds of Abrus precatorius, has documented antitumor and immunostimulatory effects in murine models. To examine possible antitumor activity against breast cancer, we established human breast tumor xenografts in athymic nude mice and intraperitoneally administered AGG. AGG inhibited tumor growth and angiogenesis as confirmed by monitoring the expression of Ki-67 and CD-31, respectively. In addition, TUNEL positive cells increased in breast tumors treated with AGG suggesting that AGG mediates anti-tumorigenic activity through induction of apoptosis and inhibition of angiogenesis. On a molecular level, AGG caused extrinsic apoptosis through ROS generation that was AKT-dependent in breast cancer cells, without affecting primary mammary epithelial cells, suggesting potential cancer specificity of this natural compound. In addition, using HUVECs, AGG inhibited expression of the pro-angiogenic factor IGFBP-2 in an AKT-dependent manner, reducing angiogenic phenotypes both in vitro and in vivo. Overall, the present results establish that AGG promotes both apoptosis and anti-angiogenic activities in human breast tumor cells, which might be exploited for treatment of breast and other cancers.

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“…By establishing a Bcl-2 over-expressing HCC cell line, Hu et al demonstrated that Bcl-2 stimulates the survival of hepatocellular cells against plant lectin ricin insult [33] . The antineoplastic efficiency of Momordica charantia lectin against nasopharyngeal carcinoma progressed through cytochrome c released from damaged mitochondria followed by the activation of caspases 8, 9 and 3, thereby increasing the production of cleaved PARP and resulting in DNA fragmentation and apoptosis [31,32] .…”

Section: Discussionmentioning

confidence: 99%

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

et al. 2014

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Aim: Abrus agglutinin (AGG) from the seeds of Indian medicinal plant Abrus precatorius belongs to the class II ribosome inactivating protein family. In this study we investigated the anticancer effects of AGG against human hepatocellular carcinoma in vitro and in vivo. Methods: Cell proliferation, DNA fragmentation, Annexin V binding, immunocytofluorescence, Western blotting, caspase activity assays and luciferase assays were performed to evaluate AGG in human liver cancer cells HepG2. Immunohistochemical staining and TUNEL expression were studied in tumor samples of HepG2-xenografted nude mice.

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Crabs Eye (Abrus precatorius) Seed and Its Immunomodulatory and Antitumor Properties

Cited by 6 publications

References 16 publications

Abrus agglutinin promotes irreparable DNA damage by triggering ROS generation followed by ATM‐p73 mediated apoptosis in oral squamous cell carcinoma

Abrus agglutinin promotes irreparable DNA damage by triggering ROS generation followed by ATM‐p73 mediated apoptosis in oral squamous cell carcinoma

Abrus agglutinin is a potent anti‐proliferative and anti‐angiogenic agent in human breast cancer

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

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