Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling

Wu, Jun-I; Lin, Yi‐Pei; Tseng, Chung‐Shi; Chen, Hui-Jane; Wang, Lu‐Hai

doi:10.1038/s41598-018-37443-4

Cited by 65 publications

(48 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have linked known cancer drivers to RBP dysregulation. For example, the oncogene crabp2 interacts with the RBP HuR to promote metastasis of lung cancer cells by regulating integrin β1/FAK/ERK signaling (Wu et al, 2019). Transforming growth factor-β (TGF-β) induces the expression of RNA-binding motif protein 38 (RBM38) in breast cancer, which promotes epithelial-to-mesenchymal transition by regulating the zonula occludens-1 transcript (Wu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of the Functions and Prognostic Values of RNA Binding Proteins in Lung Squamous Cell Carcinoma

Xue

Gao

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Lung cancer is the leading cause of cancer-related deaths worldwide. Dysregulation of RNA binding proteins (RBPs) has been found in a variety of cancers and is related to oncogenesis and progression. However, the functions of RBPs in lung squamous cell carcinoma (LUSC) remain unclear. In this study, we obtained gene expression data and corresponding clinical information for LUSC from The Cancer Genome Atlas (TCGA) database, identified aberrantly expressed RBPs between tumors and normal tissue, and conducted a series of bioinformatics analyses to explore the expression and prognostic value of these RBPs. A total of 300 aberrantly expressed RBPs were obtained, comprising 59 downregulated and 241 upregulated RBPs. Functional enrichment analysis indicated that the differentially expressed RBPs were mainly associated with mRNA metabolic processes, RNA processing, RNA modification, regulation of translation, the TGF-beta signaling pathway, and the Toll-like receptor signaling pathway. Nine RBP genes (A1CF, EIF2B5, LSM1, LSM7, MBNL2, RSRC1, TRMU, TTF2, and ZCCHC5) were identified as prognosis-associated hub genes by univariate, least absolute shrinkage and selection operator (LASSO), Kaplan-Meier survival, and multivariate Cox regression analyses, and were used to construct the prognostic model. Further analysis demonstrated that high risk scores for patients were significantly related to poor overall survival according to the model. The area under the time-dependent receiver operator characteristic curve of the prognostic model was 0.712 at 3 years and 0.696 at 5 years. We also developed a nomogram based on nine RBP genes, with internal validation in the TCGA cohort, which showed a favorable predictive efficacy for prognosis in LUSC. Our results provide novel insights into the pathogenesis of LUSC. The nine-RBP gene signature showed predictive value for LUSC prognosis, with potential applications in clinical decision-making and individualized treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of the Functions and Prognostic Values of RNA Binding Proteins in Lung Squamous Cell Carcinoma

Xue

Gao

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Yu S et al report that CRABP2 promotes the invasion of pancreatic cancer cells through stabilizing the interleukin 8 expression [34]. Wu J et al demonstrate that CRABP2 enhances lung cancer metastasis by HuR and integrin β1/FAK/ERK signaling [35]. These results highlight the oncogenic role of CRABP2 in the progression of cancers.…”

Section: Discussionmentioning

confidence: 93%

Dezocine inhibits cell proliferation, migration and invasion by targeting CRABP2 in ovarian cancer

Zhang¹,

R²,

Ma³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Previous studies have shown that some anesthesia drugs can inhibit tumor growth and metastasis. As a clinical anesthetic drug, dezocine has been reported to play an important role in immune function. However, the effects of dezocine on ovarian cancer cell growth and metastasis are not fully understood. Results: In this study, we found that dezocine dose-dependently inhibited the viability of ovarian cancer ES-2 and SKOV3 cells. Dezocine suppressed the migration and invasion abilities of ovarian cancer cells and promoted apoptosis. Moreover, the Akt/mTOR signaling pathway was also inhibited by dezocine. Furthermore, the mechanism study showed that dezocine could significantly inhibit the expression of CRABP2, and CRABP2 overexpression reversed the inhibitory effects of dezocine on ovarian cancer cell proliferation and migration. Conclusions: In conclusion, dezocine has significant anti-tumor effects on the growth and metastatic potential of ovarian cancer cells, and CRABP2 functions as a downstream effector of dezocine.

show abstract

“…In addition, activation of ITGA7 is correlated with metastasis, recurrence, and reduced survival in colorectal cancer patients . Although the function of ITGA7 is still unclear in NSCLC, there has been study illustrating that the activation of integrin beta 1 (ITGB1), with whom ITGA7 binds and forms a heterodimeric adhesion receptor, and its downstream signaling molecules FAK and ERK promote resistance to apoptosis, enhance cell migration, and invasion of lung cancer cells . In addition, the FAK/AKT singling pathway, which is activated by ITGA7 is responsible for lung cancer cell migration and invasion as well .…”

Section: Discussionmentioning

confidence: 99%

Correlation of integrin alpha 7 with clinicopathological characteristics and survival profiles, as well as its regulatory role in cell proliferation, apoptosis, and stemness in non‐small‐cell lung cancer

Xia

Chen

Xiao

2019

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background The present study aimed to evaluate the correlation of integrin alpha 7 (ITGA7) with patients' clinicopathological characteristics and survival profiles, as well as its influence on cell proliferation, apoptosis, and stemness in non–small‐cell lung cancer (NSCLC). Methods A total of 397 NSCLC patients underwent surgical resection were included, and ITGA7 was measured in tumor tissues and adjacent tissues by immunohistochemistry. patients' clinical data were extracted from database, and follow‐up records were reviewed. In cellular experiments, expression of ITGA7 was measured in NSCLC cell lines and normal human lung epithelial cell line by RT‐qPCR. The influence of ITGA7 on cell activity was assessed by transfecting overexpression plasmids and knockdown plasmids of ITGA7 into A549 cells. Results Integrin alpha 7 was upregulated in tumor tissues compared with the adjacent tissues of NSCLC patients. Patients with ITGA7 high expression presented poorer pathological differentiation, larger tumor size, and more advanced TNM stage compared with patients with ITGA7 low expression. For survival profiles, both disease‐free survival and overall survival were shorter in ITGA7 high expression patients compared with ITGA7 low expression patients. In cellular experiments, ITGA7 was upregulated in NCI‐H1650, A549, HCC‐827, and NCI‐H1299 cells compared with normal human lung epithelial cells BEAS‐2B. In addition, ITGA7 promoted cell proliferation, inhibited cell apoptosis, and facilitated cell stemness in A549 cells. Conclusion Integrin alpha 7 correlates with poor clinicopathological characteristics and survival profiles, and it promotes cell proliferation, stemness but suppresses cell apoptosis in NSCLC.

show abstract

Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling

Cited by 65 publications

References 60 publications

Integrated Analysis of the Functions and Prognostic Values of RNA Binding Proteins in Lung Squamous Cell Carcinoma

Integrated Analysis of the Functions and Prognostic Values of RNA Binding Proteins in Lung Squamous Cell Carcinoma

Dezocine inhibits cell proliferation, migration and invasion by targeting CRABP2 in ovarian cancer

Correlation of integrin alpha 7 with clinicopathological characteristics and survival profiles, as well as its regulatory role in cell proliferation, apoptosis, and stemness in non‐small‐cell lung cancer

Contact Info

Product

Resources

About