Cr(VI) reduction by Bacillus sp. isolated from chromium landfill

Liu, Yunguo; Xu, Wenji; Zeng, Guangming; Li, Xin; Gao, Hui

doi:10.1016/j.procbio.2006.04.020

Cited by 147 publications

(88 citation statements)

References 26 publications

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“…XW-4 was tolerant to and reduced 1.9 mM Cr(VI). Chromate reduction by the strain was significantly enhanced by the presence of glucose (Liu et al 2006). In another study, suspended cultures of B. cereus GIDM20, B. fusiformis GIDM22 and B. sphearicus GIDM64 exhibited more than 85% reduction of 1 mM Cr(VI) within 30 h. The activity was found to be mainly associated with the soluble fraction of cells, expressed constitutively and unaffected by presence of different metal ions except Hg 2?…”

Section: Bacterial Cr(vi) Reductionmentioning

confidence: 92%

Remediation of chromium contaminants using bacteria

Kanmani¹,

Aravind²,

Preston

2011

Int. J. Environ. Sci. Technol.

123

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Chromium contaminants emanating from industrial activities pose a significant threat to human's wellbeing. Chromium (III) and Chromium (VI) are the forms in which they are commonly encountered, of which the trivalent form is relatively benign. Hence, biological reduction of hexavalent chromium has been widely explored by researchers, yielding fruitful outcomes, opening up exciting avenues and also throwing up new challenges. This article attempts to review this area of research. Microbes, especially bacteria capable of Chromium (VI) reduction, belonging to a heterogeneous group have been isolated from contaminated sites. They exhibit plasmid-mediated chromate resistance and the reduction is enzymatically mediated. Reduction studies have been carried out with free and immobilized enzymes as well as whole cells. Experiments have been carried out in specifically designed bioreactors operated in batch and continuous modes. Although significant progress has been made, much needs to be done for its successful in situ application as the organism may not withstand the Chromium concentration or may be impeded by the presence of other toxicants. With molecular engineering, it may be possible to derive strains with improved performance even under stressful field conditions.

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Section: Bacterial Cr(vi) Reductionmentioning

confidence: 92%

Remediation of chromium contaminants using bacteria

Kanmani¹,

Aravind²,

Preston

2011

Int. J. Environ. Sci. Technol.

123

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“…Strains of genus Bacillus are known to tolerate and reduce Cr (VI) (Campos et al, 1995;Liu et al, 2006;Soni et al, 2013). The tolerance level of Cr (VI) for our newly isolated strain of Bacillus cereus was 3.4 mM; which is more than other strain of Bacillus cereus those have been known to tolerate 2 mM Cr (VI) (Sarangi and Khrishan, 2008).…”

Section: Discussionmentioning

confidence: 76%

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Nguema

Luo²,

Mounir³

et al. 2017

IJB

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An efficient Cr (VI)-resistant and reducing bacterial strain was successfully isolated in dewatering sludge collected from a sewage treatment plant, and characterized in vitro Cr (VI) reduction through a reductase enzyme. Phylogenetic analysis using 16S rDNA gene sequencing revealed that the newly isolated strain namely Pf-1 was closely related to Bacillus cereus. The strain almost reduced 0.17 mM Cr (VI) within 24h incubation and the presence of different substrates such as glucose, sucrose, or acetate significantly enhanced the reduction rate of Cr (VI) to Cr (III). However, addition of the same substrate at the stationary phase of the microbial growth increased the reduction rate as well as bacterial growth. Additionally, raising the concentration of thiosulfate in the medium doubled the reduction rate under similar conditions. Assay with different fractions of the cells demonstrated that the reductase enzyme activity was mainly associated with the cytoplasmic fraction. The maximum activity was 23.3 µM h -1 mg -1 protein and was obtained at the Cr (VI) concentration of 2 mM. The promising strain can be efficiently employed for bioremediation of Cr (VI) polluted sites.

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“…Most likely, bacterial growth and Cr (VI)-induced damage are competing processes, and bacteria can cope with Cr (VI) exposure only as long as metabolizable carbon sources are available. Liu et al (2006) noticed that this phenomenon might be explained as an increased time period for adaptation or repair during the exposure to high level of Cr (VI) in the growth medium.…”

Section: Discussionmentioning

confidence: 99%