Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris

Franco, Lauren; Steinbeisser, Sadie; Zane, Grant M.; Wall, Judy D.

doi:10.1007/s00253-017-8724-4

Cited by 24 publications

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“…shown for Desulfovibrio, the mechanism (Type I or II) under the nutrient deprived state is not 95 known. We recently demonstrated that nutrient ratios impacted metal interactions (i.e., Cr(VI) 96 sensitivity) in Desulfovibrio (Franco et al 2018), and therefore, hypothesized that nutrient 97 imbalance would impact reduction-oxidation reactions in Desulfovibrio biofilms grown on a metal 98 surface.…”

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Bulk phase resource ratio alters carbon steel corrosion rates and endogenously produced extracellular electron transfer mediators in a sulfate-reducing biofilm

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Desulfovibrio alaskensis G20 biofilms were cultivated on 316 steel, 1018 steel, or borosilicate 37 glass under steady-state conditions in electron-acceptor limiting (EAL) and electron-donor 38 limiting (EDL) conditions with lactate and sulfate in a defined medium. Increased corrosion was observed on steel under EDLconditions compared to 316 steel, and biofilms on 1018 carbon 40 steel under the EDL condition had at least 2-fold higher corrosion rates compared to the EAL 41 condition. Protecting the 1018 metal coupon from biofilm colonization significantly reduced 42 corrosion, suggesting that the corrosion mechanism was enhanced through attachment between the 43 material and the biofilm. Metabolomic mass spectrometry analyses demonstrated an increase in a 44 flavin-like molecule under the 1018 EDL condition and sulfonates under the 1018 EAL condition. 45 These data indicate the importance of S-cycling under the EAL condition and the EDL is 46 associated to increased biocorrosion via indirect extracellular electron transfer mediated by 47 endogenously produced flavin-like molecules. 48 58 deliverability. Adding to scale of the problem, MIC occurs under a wide variety of environmental 59 conditions, including marine, freshwater and terrestrial locations. 60 MIC can involve a variety of different microorganisms that include sulfate-reducing bacteria (SRB) and iron-reducing bacteria (IRB) (Little et al. 2007, Enning and Garrelfs 2014, 62 Bonifay et al. 2017). Together with SRBs and IRBs, microbial consortia are typically involved in two mechanisms of MIC: EET-MIC (extracellular electron transfer, Type I), cross membrane 64 electron transfer (indirect and/or direct) (Kato 2016 and references therein), and/or metabolite-65 MIC (Type II), biocorrosion caused by secreted metabolites (e.g., H + , organic acids, sulfides) as 66 opposed to chemical corrosion which can refer to direct metal-oxidant interactions (Li et al. 2018, 67 Kannan et al 2018). Both EET-MIC and M-MIC are electrochemical corrosion processes (Li et 68 al. 2018, Dinh et al. 2004), and different EET mechanisms can promote the transfer of electrons 69 to or from extracellular solid compounds (Gralnick and Newman 2007, Kato 2016). The process 70 of EET can be mediated directly with metal surfaces via cellular connections and/or conductive 71 extracellular structures (e.g., Gorby et al. 2006) or indirectly via diffusible redox molecules 72 (Watanabe et al. 2009). The goal of this study was to elucidate nutrient ratio impacts on potential 73 M-MIC and/or EET-MIC mechanisms during initial biofilm formation under continuous growth 74 conditions in a defined growth medium. 75Extracellular electron transfer, which is shown to enhance MIC, is now recognized as a 76 more widespread microbial phenotype and suggests that EET-MIC could be a major mechanism 77 for biocorrosion world-wide (Nealson and Saffarini, 1994, Kato 2016, Huang et al. 2018.78 Previous work postulated that some SRBs contribute to MIC under various growth conditions 79 through Fe 0 oxidation via inte...

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Bulk phase resource ratio alters carbon steel corrosion rates and endogenously produced extracellular electron transfer mediators in a sulfate-reducing biofilm

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“…The return of the upp gene restores sensitivity, providing a counterselectable gene. JW710 also allows site-directed mutations ( 13 ) and multiple deletions to be created without an accumulation of selectable markers ( 12 , 14 ). The plasmids, pSC27 ( 15 ), pMO719 ( 16 ), pMO9075 ( 11 ), and pMO746 ( 17 ), with features used in various strain constructions, are available from https://www.addgene.org/Judy_Wall/ .…”

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confidence: 99%

Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

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Juba

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“…), 11.5 cm (height) glass column packed with approximately 760 (6 mm diameter) glass beads and hydraulic loading rate of 40 mL/h (across a pore volume of 60 mL) to achieve a hydraulic retention time of 1.5 h. Reactors operated under optimal loading conditions towards steadystate operation were challenged by NADH inhibitors, cytochrome-b inhibitors and ATPase uncouplers at different times of operation to evaluate the electron pathway mechanisms in the organisms. Continuation of Cr(VI) reduction activity after blocking cytochrome-b activity would indicate Cr(VI) reduction via NADH-dehydrogenase [87] or Cr(VI) reduction connected to sulphate shuttle mechanisms that can also transport chromate (CrO 4 2−…”

Section: Example Of Case Study For Lab-scale and Pilot-scale Evaluationmentioning

confidence: 99%

Section: Example Of Case Study For Lab-scale and Pilot-scale Evaluationmentioning

confidence: 99%

“…) into the cytoplasm of the cell [30,87,88]. Cr(VI) reduction in the presence of uncouplers could imply membrane associated Cr(VI) reduction pathway via the membrane electron phosphorylation transport system [87]. The following parameters were determined for the experiment; Cr(VI), total Cr, Cr(III)-OH 3 , cell surface reactions, • Cr-absorption and Cr-efflux of chrA and chrAB engineered strains were significantly stronger than the control strain.…”

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Bacterial Reduction of Cr(VI): Operational Challenges and Feasibility

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Purpose of Review Hexavalent chromium, Cr(VI), and trivalent chromium, Cr(III), are two chromium compounds with practical importance due to their high occurrence and solubility in the environment. Current Cr(VI) treatment techniques involve chemical reduction of Cr(VI) to Cr(III), which posed serious threat to workers and environment notably from long exposure and toxic fumes.Recent Findings Numerous reports have demonstrated the feasibility of using biological processes for the treatment of Cr(VI) industrial effluents by either pure culture or a consortium of Cr(VI)-reducing bacteria, with various degrees of success. Among issues to be considered include high cost of nutrient for the bacteria, low Cr resistant-reducing ability of environmental isolates, difficulty in scaling up finding in the laboratory to pilot scale and on-site application as well as the understanding on the dynamic underlying mechanisms for bacterial Cr(VI) reduction. Summary This review highlights cytotoxicity and genotoxicity properties of Cr(VI), which form the biggest motivation for continuous development in the field of Cr(VI) treatment technologies, latest finding in aerobic and anaerobic bacterial reduction of Cr(VI), operational challenges for bacterial Cr(VI) reduction, and some examples for laboratory-scale and pilot-scale evaluation of free and immobilized (biofilm) cells of Cr(VI) resistant-reducing bacteria.

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Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris

Cited by 24 publications

References 44 publications

Bulk phase resource ratio alters carbon steel corrosion rates and endogenously produced extracellular electron transfer mediators in a sulfate-reducing biofilm

Bulk phase resource ratio alters carbon steel corrosion rates and endogenously produced extracellular electron transfer mediators in a sulfate-reducing biofilm

Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

Bacterial Reduction of Cr(VI): Operational Challenges and Feasibility

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