Background: Thiourea derivatives act as promising chemosensors for sensing transition metal ions. 1-(2-hydroxyphenyl) thiourea (HPTU) is one such chromophore that has potential for metal ion sensing. The current investigation reports the sensing of chromium species using transition metal-oxo-based reaction of 1,2-hydroxyphenylthiourea. Methods: The catalytic effect of chromium (III) and chromium (VI) on the oxidation of HPTU was studied. The reaction was followed spectrofluorimetrically by measuring the fluorescence intensities of the reaction product at λ ex = 416 and λ em = 520 nm, respectively.