CPPU may induce gibberellin-independent parthenocarpy associated with PbRR9 in ‘Dangshansu’ pear

Liang

et al. 2021

Horticulture Research

Self Cite

Numerous environmental and endogenous signals control the highly orchestrated and intricate process of plant senescence. Ethylene, a well-known inducer of senescence, has long been considered a key endogenous regulator of leaf and flower senescence, but the molecular mechanism of ethylene-induced ovule senescence has not yet been elucidated. In this study, we found that blockage of fertilization caused ovule abortion in the pear cultivar ‘1913’. According to transcriptome and phytohormone content data, ethylene biosynthesis was activated by pollination. At the same time, ethylene overaccumulated in ovules, where cells were sensitive to ethylene signals in the absence of fertilization. We identified a transcription factor in the ethylene signal response, ethylene-insensitive 3-like (EIL1), as a likely participant in ovule senescence. Overexpression of PbEIL1 in tomato caused precocious onset of ovule senescence. We further found that EIL1 could directly bind to the promoter of the SENESCENCE-ASSOCIATED CYSTEINE PROTEINASE 1 (PbCysp1) gene and act upstream of senescence. Yeast one-hybrid and dual-luciferase assays revealed the interaction of the transcription factor and the promoter DNA sequence and demonstrated that PbEIL1 enhanced the action of PbCysp1. Collectively, our results provide new insights into how ethylene promotes the progression of unfertilized ovule senescence.

Section: Introductionmentioning

confidence: 99%

PbEIL1 acts upstream ofPbCysp1to regulate ovule senescence in seedless pear

Wang

Liang

et al. 2021

Horticulture Research

Self Cite

“…After centrifugation at 12,000 rpm for 20 min at 4 °C, the supernatant was transferred and then dried under a nitrogen gas. The dried residues were diluted in 200 μL of 50% methanol for HPLC analysis according to a previously described method …”

Section: Methodsmentioning

confidence: 99%

“…The dried residues were diluted in 200 μL of 50% methanol for HPLC analysis according to a previously described method. 39 The soluble sugar components were measured according to previous research with a minor revision. 40 In brief, approximately, 300 mg leaves were ground with liquid nitrogen and soaked with 5 mL of 80% ethanol twice at 85 °C for 30 min each time.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Accumulation of Galactinol and ABA Is Involved in Exogenous EBR-Induced Drought Tolerance in Tea Plants

Xiao

et al. 2022

J. Agric. Food Chem.

Drought stress severely limits growth and causes losses in the yield of tea plants. Exogenous application of 24-epibrassinolide (EBR) positively regulates drought responses in various plants. However, whether EBR could contribute to drought resistance in tea plants and the underlying mechanisms has not been investigated. Here, we found that EBR application is beneficial for the drought tolerance of tea plants. The transcriptome results revealed that EBR could contribute to tea plant drought resistance by promoting galactinol and abscisic acid (ABA) biosynthesis gene expression. The content of galactinol was elevated by EBR and EBR-responsive CsDof1.1 positively regulated the expression of the galactinol synthase genes CsGolS2-1 and CsGolS2-2 to contribute to the accumulation of galactinol by directly binding to their promoters. Moreover, exogenous EBR was found to elevate the expression of genes related to ABA signal transduction and stomatal closure regulation, which resulted in the promotion of stomatal closure. In addition, EBR-responsive CsMYC2-2 is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2 to activate their expression. In summary, findings in this study provide knowledge into the transcriptional regulatory mechanism of EBR-induced drought resistance in tea plants.

“…CKs increase auxin concentrations, leading to parthenocarpic fruit in Pyrus spp. (pear) ( Cong et al., 2020 ), while ETH decreases bioactive GA concentrations, inhibiting parthenocarpic fruit formation in tomato ( Fos et al., 2000 ; Pascual et al., 2009 ; Shinozaki et al., 2015 ). However, a clear and systematic framework underlying the effects of various hormones on parthenocarpic fruit growth is still lacking.…”

Section: Reproductive Growthmentioning

confidence: 99%

Effects of gibberellins on important agronomic traits of horticultural plants

Zhao²,

Sun³

et al. 2022

Front. Plant Sci.

Horticultural plants such as vegetables, fruits, and ornamental plants are crucial to human life and socioeconomic development. Gibberellins (GAs), a class of diterpenoid compounds, control numerous developmental processes of plants. The roles of GAs in regulating growth and development of horticultural plants, and in regulating significant progress have been clarified. These findings have significant implications for promoting the quality and quantity of the products of horticultural plants. Here we review recent progress in determining the roles of GAs (including biosynthesis and signaling) in regulating plant stature, axillary meristem outgrowth, compound leaf development, flowering time, and parthenocarpy. These findings will provide a solid foundation for further improving the quality and quantity of horticultural plants products.