CPPED1-targeting microRNA-371a-5p expression in human placenta associates with spontaneous delivery

Abstract

MicroRNAs (miRNAs) are important regulators of gene expression, and their expression is associated with many physiological conditions. Here, we investigated potential associations between expression levels of miRNAs in human placenta and the onset of spontaneous term birth. Using RNA sequencing, we identified 54 miRNAs differentially expressed during spontaneous term labor compared to elective term births. Expression levels of 23 miRNAs were upregulated, whereas 31 were downregulated at least 1.5-fold. The upregulated miRNA miR-371a-5p putatively targets CPPED1, expression of which decreases during spontaneous birth. We used a luciferase reporter-based assay to test whether a miR-371a-5p mimic affected translation when it bound to the 3 0 untranslated region of CPPED1. In this setting, the miR-371a-5p mimic resulted in lower luciferase activity, which suggests that miR-371a-5p regulates levels of CPPED1. In conclusion, inversely correlated levels of miR-371a-5p and CPPED1 suggest a role for both in spontaneous delivery.

The potential function of SP1 and CPPED1 in restenosis after percutaneous coronary intervention

“…Meanwhile, it was found via bioinformatics analysis in our research that there existed binding relationship between SP1 and another molecule calcineurin‐like phosphoesterase domain containing 1 (CPPED1). CPPED1 is a member of calcineurin‐like metallophosphoesterase superfamily involving in the timing of birth and spontaneous delivery 13,14 . It was disclosed that CPPED1 might be associated with the pathogenesis of osteonecrosis of the femoral head 15 .…”

“…CPPED1 is a member of calcineurin-like metallophosphoesterase superfamily involving in the timing of birth and spontaneous delivery. 13,14 It was disclosed that CPPED1 might be associated with the pathogenesis of osteonecrosis of the femoral head. 15 Even though the impact of CPPED1 in restenosis after PCI has barely been discussed before, it was known from the microarray data in our study that CPPED1 and SP1 were both highly expressed in the serum of patients with restenosis.…”

Reference Genes for qPCR-Based miRNA Expression Profiling in 14 Human Tissues

“…Liang et al [ 127 ] analyzed the expression levels of 345 miRNAs in 40 normal tissues and found miR-30e-5p, miR-92a-3p, and miR-423-3p to have the least variable expression. Although our study has identified promising reference genes that can be universally used in different tissues, literature analysis has demonstrated that miRNA-16-5p, miRNA-103a-3p, and miRNA-191-5p are most commonly used as universal reference genes [ 29 , 31 , 35 , 42 , 89 , 99 , 110 ].…”