CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection against<i>Burkholderia pseudomallei</i>Infection in BALB/c Mice

Chen, Yao-Shen; Hsiao, Yu-Sheng; Lin, Hsin‐Hung; Yin, Lei; Chen, Ya-Lei

doi:10.1128/iai.74.3.1699-1705.2006

Cited by 51 publications

(61 citation statements)

References 41 publications

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“…32 Early results from ongoing clinical studies indicate that CpG sequences are well tolerated and improve the immune response to microbial vaccines: 29 exogenous CpG motifs added to the fliC gene of Burkholderia pseudomallei enhance flagellin immunogenicity and provide protection against melioidosis. 33 Furthermore, while certain CpG sequences may induce . 29 Selective methylation of the cytosine of the plasmid CpG dinucleotides reduces its ability to stimulate in vitro cytokine production and in vivo antibody or cytotoxic T lymphocyte production.…”

Section: Toll-like Receptor Function In Muscle Cellsmentioning

confidence: 99%

Skeletal muscle cells: from local inflammatory response to active immunity

et al. 2010

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The skeletal muscles are the major living component of the human body. They are constituted by stable cells, the myofibres, and by adult multipotent stem cells, the satellite cells, which can multiply to regenerate and repair the damaged tissues. Injections of DNA in muscle cells have been used to produce recombinant proteins with opposite goals: somatic reparation of genetic defects, which needs to elicit no inflammatory or immune response, and DNA vaccination, which needs a robust immune response. Because of possible therapeutical interventions, a growing body of information is being produced dealing with every aspect of the myofibres during inflammatory and autoimmune responses: skeletal muscle-antigen presenting cell (APC) interaction and intrinsic APC capabilities of myoblasts and myocytes, the response to released cytokines and their endogenous production, the regulation of Toll-like receptors and major histocompatibility complex expression. According to these data, the muscle tissue is now emerging no longer as a passive bystander, but more as an active player that, when correctly manipulated, can drive tolerance or immunization to these de novo produced proteins. In the present review, we summarize the recent developments on the control of muscle immune function.

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Section: Toll-like Receptor Function In Muscle Cellsmentioning

confidence: 99%

Skeletal muscle cells: from local inflammatory response to active immunity

et al. 2010

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“…To improve the efficacy of the polysaccharide vaccines, it may be necessary to identify additional protein antigens that also afford protection against melioidosis. To our knowledge, only immunization with a DNA vaccine encoding the flagellin protein FliC (8,9) or sera raised against FliC (5) has been shown to afford any protection against B. pseudomallei infection in mice.…”

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confidence: 99%

Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

et al. 2007

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“…However, antibodies to BipB, BipC, and BipD have been detected in sera of melioidosis patients (11,28). Furthermore, a DNA vaccine encoding the flagellin protein and carrying CpG motifs enhanced resistance of immunized mice to B. pseudomallei (5,6). Owing to the incomplete protection afforded by candidate antigens to date, it is necessary to search for additional proteins for vaccine development.…”

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confidence: 99%

Burkholderia pseudomalleiProteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T CellsIn Vitro

et al. 2011

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Melioidosis is a severe infectious disease caused by the saprophytic facultative intracellular pathogen Burkholderia pseudomallei. The disease is endemic in Southeast Asia and Northern Australia, and no effective vaccine exists. To describe human cell-mediated immune responses to B. pseudomallei and to identify candidate antigens for vaccine development, the ability of antigen-pulsed monocyte-derived dendritic cells (moDCs) to trigger autologous T-cell responses to B. pseudomallei and its products was tested. moDCs were prepared from healthy individuals exposed or not exposed to B. pseudomallei, based on serological evidence. These were pulsed with heat-killed B. pseudomallei or purified antigens, including ABC transporters (LolC, OppA, and PotF), Bsa type III secreted proteins (BipD and BopE), tandem repeat sequence-containing proteins (Rp1 and Rp2), flagellin, and heat shock proteins (Hsp60 and Hsp70), prior to being mixed with autologous T-cell populations. After pulsing of cells with either heat-killed B. pseudomallei, LolC, or Rp2, coculturing the antigen-pulsed moDCs with T cells elicited gamma interferon production from CD4 ؉ T cells from seropositive donors at levels greater than those for seronegative donors. These antigens also induced granzyme B (cytotoxic) responses from CD8 ؉ T cells. Activation of antigen-specific CD4 ؉ T cells required direct contact with moDCs and was therefore not dependent on soluble mediators. Rp peptide epitopes recognized by T cells in healthy individuals were identified. Our study provides valuable novel data on the induction of human cell-mediated immune responses to B. pseudomallei and its protein antigens that may be exploited in the rational development of vaccines to combat melioidosis.

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CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection againstBurkholderia pseudomalleiInfection in BALB/c Mice

Cited by 51 publications

References 41 publications

Skeletal muscle cells: from local inflammatory response to active immunity

Skeletal muscle cells: from local inflammatory response to active immunity

Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

Burkholderia pseudomalleiProteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T CellsIn Vitro

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