2017
DOI: 10.1186/s13148-017-0386-7
|View full text |Cite
|
Sign up to set email alerts
|

CpG location and methylation level are crucial factors for the early detection of oral squamous cell carcinoma in brushing samples using bisulfite sequencing of a 13-gene panel

Abstract: BackgroundOral squamous cell carcinoma (OSCC) is usually diagnosed at an advanced stage and is commonly preceded by oral premalignant lesions. The mortality rates have remained unchanged (50% within 5 years after diagnosis), and it is related to tobacco smoking and alcohol intake. Novel molecular markers for early diagnosis are urgently needed. The purpose of this study was to evaluate the diagnostic value of methylation level in a set of 18 genes by bisulfite next-generation sequencing.MethodsWith minimally i… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

6
115
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
7
2

Relationship

4
5

Authors

Journals

citations
Cited by 53 publications
(121 citation statements)
references
References 65 publications
(78 reference statements)
6
115
0
Order By: Relevance
“…Bisulphite treatment of genomic DNA was performed using the EZ DNA Methylation‐Lightning™ Kit (Zymo Research, Irvine, CA, USA; code D5031), according to the manufacturer's protocol. DNA methylation analysis was performed by bisulphite‐NGS for BRCA1 , BRCA2 , PDCD1 , PD‐L1 , CDH1 , PRC1 , MAGEA2 , MAGEA11 , TERT and UXT genes, as previously described . Briefly, a two‐step library was prepared following the same protocol described above for mutation analysis.…”
Section: Methodsmentioning
confidence: 99%
“…Bisulphite treatment of genomic DNA was performed using the EZ DNA Methylation‐Lightning™ Kit (Zymo Research, Irvine, CA, USA; code D5031), according to the manufacturer's protocol. DNA methylation analysis was performed by bisulphite‐NGS for BRCA1 , BRCA2 , PDCD1 , PD‐L1 , CDH1 , PRC1 , MAGEA2 , MAGEA11 , TERT and UXT genes, as previously described . Briefly, a two‐step library was prepared following the same protocol described above for mutation analysis.…”
Section: Methodsmentioning
confidence: 99%
“…Rigorous independent validation is crucial to derive reliable genetic and epigenetic makers or marker panels, particularly for candidates discovered in genome-wide approaches (57,60). In only eight of the studies validations in independent samples were performed.…”
Section: Discussionmentioning
confidence: 99%
“…Brushing cell collection was performed prior to any cancer treatment in the tumor mass. Two hundred forty-five CpG islands from a set of 13 previously described methylated genes in OSCC (ZAP70, KIF1A, LRRTM1, PARP15, FLI1, NTM, LINC0059, EPHX3, ITGA4, MIR193, GP1BB, MIR296, TERT) [1] were investigated by bisulfite-Target Next Generation Sequencing (NGS) using MiSEQ platform (Illumina, San Diego, CA, USA).…”
Section: Methodsmentioning
confidence: 99%