CpG DNA analysis of bacterial STDs

Singer, M.; Waaij, Dewi J. de; Morré, Servaas A.; Ouburg, Sander

doi:10.1186/s12879-015-1016-7

Cited by 8 publications

(10 citation statements)

References 45 publications

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“…Experimentally, the presence of TSST-1 favors the development of septic arthritis [ 60 ]. Another important virulence factor is bacterial deoxyribonucleic acid (DNA) with non-methylated CpG motifs, which is considerably less frequent in vertebrate DNA [ 61 ]. The CpG DNA can bind to Toll-like receptor 9 (TLR9) in immune cells, leading to the production of cytokines such as IL-1β, TNF, IL-6 and IL-12 [ 62 , 63 ].…”

Section: Septic Arthritismentioning

confidence: 99%

Neutrophils: Beneficial and Harmful Cells in Septic Arthritis

Boff

Crijns

Teixeira

et al. 2018

IJMS

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Septic arthritis is an inflammatory joint disease that is induced by pathogens such as Staphylococcus aureus. Infection of the joint triggers an acute inflammatory response directed by inflammatory mediators including microbial danger signals and cytokines and is accompanied by an influx of leukocytes. The recruitment of these inflammatory cells depends on gradients of chemoattractants including formylated peptides from the infectious agent or dying cells, host-derived leukotrienes, complement proteins and chemokines. Neutrophils are of major importance and play a dual role in the pathogenesis of septic arthritis. On the one hand, these leukocytes are indispensable in the first-line defense to kill invading pathogens in the early stage of disease. However, on the other hand, neutrophils act as mediators of tissue destruction. Since the elimination of inflammatory neutrophils from the site of inflammation is a prerequisite for resolution of the acute inflammatory response, the prolonged stay of these leukocytes at the inflammatory site can lead to irreversible damage to the infected joint, which is known as an important complication in septic arthritis patients. Thus, timely reduction of the recruitment of inflammatory neutrophils to infected joints may be an efficient therapy to reduce tissue damage in septic arthritis.

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Section: Septic Arthritismentioning

confidence: 99%

Neutrophils: Beneficial and Harmful Cells in Septic Arthritis

Boff

Crijns

Teixeira

et al. 2018

IJMS

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“… 16 Another important virulence factor is bacterial deoxyribonucleic acid (DNA) with non-methylated CpG motifs, which is considerably less frequent in vertebrate DNA. 17 The CpG DNA can bind to Toll-like receptor 9 (TLR9) in immune cells, leading to the production of cytokines such as IL-1β, TNF, IL-6 and IL- 12. 17 Those various factors may predispose to the development of Deep Vein Thrombosis (DVT) caused by S. aureus induced septic arthritis.…”

Section: Discussionmentioning

confidence: 99%

“… 17 The CpG DNA can bind to Toll-like receptor 9 (TLR9) in immune cells, leading to the production of cytokines such as IL-1β, TNF, IL-6 and IL- 12. 17 Those various factors may predispose to the development of Deep Vein Thrombosis (DVT) caused by S. aureus induced septic arthritis.…”

Section: Discussionmentioning

confidence: 99%

A Case of Deep Vein Thrombosis Associated with Methicillin Sensitive Staphylococcal aureus Genu Septic Arthritis

Effendy¹,

Octora²,

Kusumaningrum³

2020

Infectious Disease Reports

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Septic arthritis caused by bacteria Gram positive Staphylococcus aureus (S. aureus) infection has been widely reported from Europe and the United States. This case presentation reported the first Staphylococcal septic arthritis, preceded by systemic erythroderma skin lesions from aregional hospital in Surabaya, Indonesia. Radiology imaging was used for confirming the defect. Joint fluid aspirate from the affected knee joint lesion was sent for joint fluid analysis and microbiology culture. The analysis showed infiltration of neutrophil inflammatory cells. S. aureus was isolated on culture and demonstrated catalase positive and coagulase positive reactions. Antimicrobial susceptibility testing was performed to determine the appropriate selection of antibiotics. Clindamycin was used for treatment and the complicated occurrence of deep vein thrombosis was treated with anticoagulant. Awareness of this disease and its progression to its complication deep vein thrombosis is required to understand the burden of this disease.

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“…Singer et al . investigated the proportions of inflammation stimulatory (5’-RRCGYY-3’) and inhibitory (5’-NCCGNN-3’ and 5’-NNCGRN-3’) sequences in the genomes of pathogenic bacteria (37). They found species dependent differences in the proportion of stimulatory and inhibitory sequences, but they did not study the inflammatory responses of each pathogen.…”

Section: Discussionmentioning

confidence: 99%

Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells

Horie¹,

Tomita²,

Oshio

et al. 2018

Preprint

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Lactococcus lactis strain Plasma (LC-Plasma) possesses strong activity of stimulating plasmacytoid dendritic cells (pDCs) via the TLR9-Myd88 pathway. To reveal the effective genome structure for pDCs stimulatory activity, we performed an in vitro screening, using randomly selected DNA fragments from the LC-Plasma genome. The results showed that CpG motifs are necessary factor for active DNA fragment, but the copy number of CpG motifs did not show strong correlation to the pDCs stimulatory activity of DNA fragment. We also found that the G+C contents of DNA fragments have significant negative effects on pDCs stimulatory activity. We also performed bioinformatics analysis of genome of lactic acid bacteria (LAB) and investigated the relation between CpG copy number in the genome and pDCs stimulatory activity. We found that strains of lactic acid bacteria (LAB) with high copy number of CpG motifs in the low-G+C region of the genome had higher probability of having high pDCs stimulatory activity. Three species, L.lactis subsp. lactis, Leuconostoc mesenteroides, and Pediococcus pentosaceus were the typical examples of high pDCs stimulatory activity LAB.ImportanceThis study provides a new perspective on the structure of DNA fragments that are able to activate pDCs via the TLR9-Myd88 pathway. The information from this study should be useful for designing new DNA fragments, including phosphodiesterbond-DNA oligomers containing CpG motifs and DNA-containing vaccines. This work also presented an in silico screening method for identifying bacterial species that are able to activate pDCs. Therefore, this study should be useful for providing data for the development of vaccine adjuvants and therapeutics for infectious and allergic diseases.

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CpG DNA analysis of bacterial STDs

Cited by 8 publications

References 45 publications

Neutrophils: Beneficial and Harmful Cells in Septic Arthritis

Neutrophils: Beneficial and Harmful Cells in Septic Arthritis

A Case of Deep Vein Thrombosis Associated with Methicillin Sensitive Staphylococcal aureus Genu Septic Arthritis

Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells

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