2017
DOI: 10.1093/bioinformatics/btx695
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Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1

Abstract: sangsubae@hanyang.ac.kr.

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Cited by 23 publications
(11 citation statements)
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“…In this study, most of the spacer used were designed via http://www.rgenome.net (Park & Bae, ; Table S2). To test the efficiency of the iCOPE method, a four‐fluorescent protein or chromoprotein (mRFP, eYFP, amilCP, and cjBlue) cassette was introduced into pUC19 to generate pUC19.S4.…”
Section: Resultsmentioning
confidence: 99%
“…In this study, most of the spacer used were designed via http://www.rgenome.net (Park & Bae, ; Table S2). To test the efficiency of the iCOPE method, a four‐fluorescent protein or chromoprotein (mRFP, eYFP, amilCP, and cjBlue) cassette was introduced into pUC19 to generate pUC19.S4.…”
Section: Resultsmentioning
confidence: 99%
“…Cpf1-Database is another web-based gRNA design tool, which includes all the available pre-searched targets of Cpf1 with 5′-TTTN-3′ PAM sequences in all coding sequence (CDS) regions within the whole genome of 12 selected organisms. Cpf1-Database provides a rapid and simple way of designing gRNAs for thousands of genes and facilitates the genome-wide screening experiment via usage of Cpf1 [37]. Cpf1-Database is updated frequently by following the recent gene/transcript/CDS annotation information and alternative PAMs of Cpf1 (Table 1).…”
Section: Guide Rna Designmentioning
confidence: 99%
“…The PAM sequence may vary according to its origin of ortholog. The Cpf1-database 1 is an online tool that offers a simple and easy way to find a potential target within the genome and design gRNA, and it can recognize AsCpf1 and LbCpf1 nucleases through DNA recognition sequences (Park and Bae, 2017).…”
Section: Introductionmentioning
confidence: 99%