cpDNA Sekansları Temelinde Trifolium L. Türlerinin Filogenetik İlişkileri

Abstract

DNA barcoding is an important molecular approach in the determination of species diversity, evaluation of phylogenetic relationships and identification of taxonomically problematic species because of insufficient diagnostic characters. It has great importance to determine the barcoding regions that will give the best result in the evaluation of phylogenetic relationships in species like Trifolium that spread over wide geographical regions in the world and show high variation accordingly. For this aim, matK and rbcL regions belonging to cpDNA was used to determine the barcoding ability and evaluate the taxonomy of the genus Trifolium. It is observed that both of barcoding regions are valuable to reveal the phylogenetic relationships, in addition to their grouping ability the species as taxonomically. However, especially the using of matK sequence informations that have high variable sites (158) and grouping ability clearly for all taxa is strongly recommended. Results provided from this study support the phylogenetic classification suggested by Ellison et al. (2006).