CPCGI confers neuroprotection by enhancing blood circulation and neurological function in cerebral ischemia/reperfusion rats

Miao, Yi; Wang, Ran; Wu, Hui; Yang, Shaofeng; Qiu, Yongming

doi:10.3892/mmr.2019.10472

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…9 In recent years, aberrant activity of Wnt signaling pathway is involved in a number of brain diseases, such as schizophrenia, 10 Alzheimer's, 11 and IS. 12 Wnt signaling has been reported to affect various biological processes in cerebral ischemic injury, including the regeneration of brain vascularization, 13 the restoration of the blood flow, 14 and stimulating NS/PC proliferation. 15 Wnt pathway is implicated in IS pathobiology and structural and functional recovery after IS.…”

Section: Introductionmentioning

confidence: 99%

Contribution of WNT2B Genetic Variants to Ischemic Stroke Occurrence in a Chinese Han Population

Yuan¹,

Fan²,

Yao

et al. 2021

Journal of Cardiovascular Pharmacology

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Section: Introductionmentioning

confidence: 99%

Contribution of WNT2B Genetic Variants to Ischemic Stroke Occurrence in a Chinese Han Population

Yuan¹,

Fan²,

Yao

et al. 2021

Journal of Cardiovascular Pharmacology

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“…Treatment of ischemic rats with compound porcine cerebroside and ganglioside injections led to the amelioration of the injury by promoting the cerebral blood flow, and simultaneously, an increased production of Wnt signaling pathway-associated proteins was noted. Taken together, the indicated results imply that Wnt signaling might play a role in the mitigation of impacts caused by cerebral ischemia [144].…”

Section: The Impact Of Wnt Signaling On Different Cell Types During Imentioning

confidence: 66%

Glia and Neural Stem and Progenitor Cells of the Healthy and Ischemic Brain: The Workplace for the Wnt Signaling Pathway

Knotek

Janečková

Kriška

et al. 2020

Genes

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Wnt signaling plays an important role in the self-renewal, fate-commitment and survival of the neural stem/progenitor cells (NS/PCs) of the adult central nervous system (CNS). Ischemic stroke impairs the proper functioning of the CNS and, therefore, active Wnt signaling may prevent, ameliorate, or even reverse the negative effects of ischemic brain injury. In this review, we provide the current knowledge of Wnt signaling in the adult CNS, its status in diverse cell types, and the Wnt pathway’s impact on the properties of NS/PCs and glial cells in the context of ischemic injury. Finally, we summarize promising strategies that might be considered for stroke therapy, and we outline possible future directions of the field.

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“…After CIRI was treated with CPCGI, we observed dose-dependent improvements in anxiety and cognitive dysfunction. Both Miao et al [30] and Wang et al [14] previously demonstrated the therapeutic effect of CPCGI on CIRI. However, previous studies have not focused on the improvement of CPCGI for psychological de cits, especially anxiety and cognitive dysfunction.…”

Section: Discussionmentioning

confidence: 99%

Compound porcine cerebroside and ganglioside injections improved anxiety and cognitive dysfunction through the NF-κB pathway in cerebral ischemia-reperfusion injury

Tian

et al. 2023

Preprint

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Background Cerebral ischemia-reperfusion injury (CIRI) can cause hippocampal inflammation and apoptosis, resulting in anxiety and cognitive dysfunction. Compound porcine cerebroside and ganglioside injections (CPCGI) are used to treat encephalopathy, but its therapeutic effects and mechanism require further exploration. Methods We screened key genes associated with the ischemic stroke (IS) and predicted their binding sites with CPCGI. We subsequently injected CPCGI into a middle cerebral artery occlusion (MCAO) rat model. 2,3,5-triphenyl tetrazolium chloride (TTC) staining and behavioral testing were performed. Hippocampal neuronal apoptosis was assessed by immunofluorescence. IL-1β, TNF-α, and NF-κB pathway were detected by Western blotting. Oxygen-glucose deprivation/reoxygenation (OGD/R)-HT-22 cells were treated CPCGI for 72 h. Cell viability and NF-κB were also evaluated. Results H2AC20, RPL3, RPL13A, RPL9, RPS23, and RPLP0 were identified as key IS genes. CPCGI was confirmed to interact with these proteins via molecular docking. Functional enrichment reflected the critical role of the NF-κB pathway in CIRI. Infarct volume and behavioral impairments in MCAO rats, especially anxiety and cognitive dysfunction, were improved by CPCGI in a dose-dependent manner. Immunofluorescence confirmed that hippocampal neuronal apoptosis was rescued by the CPCGI. Western blotting revealed that inflammation and NF-κB phosphorylation were inhibited. In vitro experiments showed that CPCGI increased the survival of OGD/R-HT-22 cells and inhibited phosphorylation of the NF-κB signaling pathway. Conclusion CPCGI can alleviate anxiety and cognitive dysfunction in CIRI and thus improve psychoneurological symptoms in patients with IS. We confirmed that CPCGI alleviate inflammation and apoptosis in the hippocampus by inhibiting the NF-κB signaling pathway.

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CPCGI confers neuroprotection by enhancing blood circulation and neurological function in cerebral ischemia/reperfusion rats

Cited by 5 publications

References 38 publications

Contribution of WNT2B Genetic Variants to Ischemic Stroke Occurrence in a Chinese Han Population

Contribution of WNT2B Genetic Variants to Ischemic Stroke Occurrence in a Chinese Han Population

Glia and Neural Stem and Progenitor Cells of the Healthy and Ischemic Brain: The Workplace for the Wnt Signaling Pathway

Compound porcine cerebroside and ganglioside injections improved anxiety and cognitive dysfunction through the NF-κB pathway in cerebral ischemia-reperfusion injury

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