Coxiella Burnetii

Gilk, Stacey D.; Voth, Daniel E.; Heinzen, Robert A.

doi:10.1002/9783527629176.ch16

Cited by 1 publication

(1 citation statement)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…). In the case of Coxiella burnetii, no exotoxins have been described, and LPS is, indeed, the only defined virulence factor (Gilk, Voth & Heinzen ). However, it should be noted that C. burnetii isolates have genes encoding a type IV secretion system predicted to deliver to the host cytosol effector proteins that mediate the formation of the parasitophorous vacuole, in which it replicates, and other cellular events (Voth et al .…”

Section: Discussionmentioning

confidence: 99%

Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis

Rojas

Galleguillos

Díaz

et al. 2013

Journal of Fish Diseases

View full text Add to dashboard Cite

Piscirickettsia salmonis is the aetiological agent of piscirickettsiosis, a disease which affects a variety of teleost species and that is particularly severe in salmonid fish. Bacterial-free supernatants, obtained from cultures of three isolates of Piscirickettsia salmonis, were inoculated in Atlantic salmon, Salmo salar L., and in three continuous cell lines in an effort to determine the presence of secretion of extracellular products (ECPs) by this microorganism. Although steatosis was found in some liver samples, no mortalities or clinical signs occurred in the inoculated fish. Clear cytotoxicity was observed after inoculation in the cell lines CHSE-214 and ASK, derived from salmonid tissues, but not in MDBK, which is of mammalian origin. The degree of cytotoxicity of the ECPs was different among the P. salmonis isolates tested. The isolate that evidenced the highest cytotoxicity in its ECPs exhibited only an intermediate virulence level after challenging fish with bacterial suspensions of the three P. salmonis isolates. Almost complete inhibition of the cytotoxic activity of ECPs was seen after proteinase K treatment, indicating their peptidic nature, and a total preclusion of the cytotoxicity was shown after their incubation at 50 °C for 30 min. Results show that P. salmonis can produce ECPs and at least some of them are thermolabile exotoxins that probably play a role in the pathogenesis of piscirickettsiosis.

show abstract

Section: Discussionmentioning

confidence: 99%