Cowitch seed agar medium - a simple new medium for identification and melanin production of Cryptococcus neoformans

Gokulshankar, S.; Babu, KL Girish; Valli, S.; Ranjitsingh, A. J. A.; Ranjith, M. S.

doi:10.1111/j.1439-0507.2009.01806.x

Cited by 8 publications

(9 citation statements)

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“…(18,27,31). When coupled with the detection of melanin production through brown pigmentation of caffeic acid agar medium (11,16,29) or caffeic acid-ferric citrate-infused paper disks (30), C. neoformans/C. gattii are easily distinguished from other Cryptococcus and yeast species.…”

Section: Discussionmentioning

confidence: 99%

“…Both urea hydrolysis and detection of melanin production are key characteristics distinguishing C. neoformans and C. gattii from other yeast and Cryptococcus spp. However, the biochemical tests developed to detect these characteristics required either lengthy incubation times (2 to 7 days) (18) or tedious preparation of media and reagents (11,16,27,(29)(30)(31). L-Canavanine glycine bromothymol blue (CGB) agar has been reported to differentiate most C. gattii isolates from C. neoformans (18).…”

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confidence: 99%

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Rapid Identification of Cryptococcus neoformans var. grubii, C. neoformans var. neoformans, and C. gattii by Use of Rapid Biochemical Tests, Differential Media, and DNA Sequencing

et al. 2011

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Rapid identification of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, andCryptococcus gattii is imperative for facilitation of prompt treatment of cryptococcosis and for understanding the epidemiology of the disease. Our purpose was to evaluate a test algorithm incorporating commercial rapid biochemical tests, differential media, and DNA sequence analysis that will allow us to differentiate these taxa rapidly and accurately. We assessed 147 type, reference, and clinical isolates, including 6 other Cryptococcus spp.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Rapid Identification of Cryptococcus neoformans var. grubii, C. neoformans var. neoformans, and C. gattii by Use of Rapid Biochemical Tests, Differential Media, and DNA Sequencing

et al. 2011

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“…These two growth media are non-selective and allow the overgrowth of fast-growing and abundant environmental molds and yeasts, which can obscure and obstruct the isolation of C. neoformans and C. gattii . Over the years, there have been numerous improvements in differential media for these two species of Cryptococcus [18,19,20]. However, selective medium for these organisms can be improved.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus gattii from environmental samples

et al. 2014

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One of the difficulties of isolating Cryptococcus neoformans and Cryptococcus gattii from environmental samples is the abundant overgrowth of other yeast and mold species on the plates. Here we report the application of benomyl to Guizotia abyssinica seed extract growth medium to improve the isolation of C. neoformans and C. gattii from environmental samples. We validated this medium by recovering C. neoformans and C. gattii from convenience soils and swabs from an endemic US region.

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“…The culture media used in this study appear to give faster results in the expression of melanin than the Mucuna pruriens agar 15 . In this medium, the authors showed that colonies of C. neoformans changed colors only between the third and fifth days of incubation.…”

Section: Microorganismsmentioning

confidence: 86%

Different culture media containing methyldopa for melanin production by Cryptococcus species

Menezes

Penatti²,

Pedroso³

2011

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Melanin production by species of Cryptococcus is widely used to characterize C. neoformans complex in mycology laboratories. This study aims to test the efficacy of methyldopa from pharmaceutical tablet as a substrate for melanin production, to compare the production of melanin using different agar base added with methyldopa, and to compare the melanin produced in those media with that produced in Niger seed agar and sunflower seed agar by C. neoformans, C. laurentii, and C. albidus. Two isolates of each species, C. neoformans, C. laurentii, and C. albidus, and one of Candida albicans were used to experimentally detect conditions for melanin production. METHODS: The following media were tested: Mueller-Hinton agar (MHA), brain and heart infusion agar (BHIA), blood agar base (BAB), and minimal medium agar (MMA), all added with methyldopa, and the media Niger seed agar (NSA) and sunflower seed agar (SSA). RESULTS: All isolates grew in most of the culture media after 24h. Strains planted on media BAB and BHIA showed growth only after 48h. All isolates produced melanin in MMA, MHA, SSA, and NSA media. CONCLUSIONS: Methyldopa in the form pharmaceutical tablet can be used as a substrate for melanin production by Cryptococcus species; minimal medium plus methyldopa was more efficient than the BAB, MHA, and BHIA in the melanin production; and NSA and SSA, followed by MMA added with methyldopa, were more efficient than other media studied for melanin production by all strains studied.

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Cowitch seed agar medium - a simple new medium for identification and melanin production of Cryptococcus neoformans

Abstract: A simple medium for identification and melanin production of Cryptococcus neoformans was developed using cowitch (Mucuna pruriens) seeds.

Cited by 8 publications

References 0 publications

Rapid Identification of Cryptococcus neoformans var. grubii, C. neoformans var. neoformans, and C. gattii by Use of Rapid Biochemical Tests, Differential Media, and DNA Sequencing

Rapid Identification of Cryptococcus neoformans var. grubii, C. neoformans var. neoformans, and C. gattii by Use of Rapid Biochemical Tests, Differential Media, and DNA Sequencing

Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus gattii from environmental samples

Different culture media containing methyldopa for melanin production by Cryptococcus species

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