COVID-19-like symptoms observed in Chinese tree shrews infected with SARS-CoV-2

Xu, Ling; Yu, Dandan; Ma, Yue; Yao, Yudong; Luo, Rong-Hua; Feng, Xiaoli; Cai, Hourong; Han, Jian; Wang, Xuehui; Li, Minghua; Ke, Changwen; Zheng, Yong‐Tang; Yao, Yong-Gang

doi:10.24272/j.issn.2095-8137.2020.053

Cited by 54 publications

(66 citation statements)

References 43 publications

(6 reference statements)

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“…Phylogenetic sequencing of their ACE-2 receptors reveals a high sequence similarity between tree shrews and humans (up to 81%) [ 105 ]. These observations have been supported by laboratory inoculations that have reported tree shrews can not only be infected by SARS-CoV-2 but also can develop minor signs of respiratory infections [ 225 , 226 ]. Despite displaying subtle pathology, it is speculated that tree shrews might serve as an intermediate host or as an asymptomatic carrier of SARS-CoV-2 [ 227 ].…”

Section: Animal Host Diversity Of Sars-cov-2mentioning

confidence: 73%

Host Diversity and Potential Transmission Pathways of SARS-CoV-2 at the Human-Animal Interface

Hedman¹,

Krawczyk

Helmy

et al. 2021

Pathogens

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Emerging infectious diseases present great risks to public health. The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing coronavirus disease 2019 (COVID-19), has become an urgent public health issue of global concern. It is speculated that the virus first emerged through a zoonotic spillover. Basic research studies have suggested that bats are likely the ancestral reservoir host. Nonetheless, the evolutionary history and host susceptibility of SARS-CoV-2 remains unclear as a multitude of animals has been proposed as potential intermediate or dead-end hosts. SARS-CoV-2 has been isolated from domestic animals, both companion and livestock, as well as in captive wildlife that were in close contact with human COVID-19 cases. Currently, domestic mink is the only known animal that is susceptible to a natural infection, develop severe illness, and can also transmit SARS-CoV-2 to other minks and humans. To improve foundational knowledge of SARS-CoV-2, we are conducting a synthesis review of its host diversity and transmission pathways. To mitigate this COVID-19 pandemic, we strongly advocate for a systems-oriented scientific approach that comprehensively evaluates the transmission of SARS-CoV-2 at the human and animal interface.

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Section: Animal Host Diversity Of Sars-cov-2mentioning

confidence: 73%

Host Diversity and Potential Transmission Pathways of SARS-CoV-2 at the Human-Animal Interface

Hedman¹,

Krawczyk

Helmy

et al. 2021

Pathogens

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“…Amplification was performed using an ABI Prism ViiA 7 Sequence Detector system (Applied Biosystems, Thermo Fisher, USA). A positive control consisting of SARS-CoV-2 RNA reference material (National Institute of Metrology, China; GBW(E)091089) and a negative control consisting of blank elution buffer were included in each reaction ( Xu et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Lower respiratory tract samples are reliable for severe acute respiratory syndrome coronavirus 2 nucleic acid diagnosis and animal model study

Tian¹,

Yang²,

Zhang³

et al. 2021

Zoological Research

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease 2019 (COVID-19) continue to impact countries worldwide. At present, inadequate diagnosis and unreliable evaluation systems hinder the implementation and development of effective prevention and treatment strategies. Here, we conducted a horizontal and longitudinal study comparing the detection rates of SARS-CoV-2 nucleic acid in different types of samples collected from COVID-19 patients and SARS-CoV-2-infected monkeys. We also detected anti-SARS-CoV-2 antibodies in the above clinical and animal model samples to identify a reliable approach for the accurate diagnosis of SARS-CoV-2 infection. Results showed that, regardless of clinical symptoms, the highest detection levels of viral nucleic acid were found in sputum and tracheal brush samples, resulting in a high and stable diagnosis rate. Anti-SARS-CoV-2 immunoglobulin M (IgM) and G (IgG) antibodies were not detected in 6.90% of COVID-19 patients. Furthermore, integration of nucleic acid detection results from the various sample types did not improve the diagnosis rate. Moreover, dynamic changes in SARS-CoV-2 viral load were more obvious in sputum and tracheal brushes than in nasal and throat swabs. Thus, SARS-CoV-2 nucleic acid detection in sputum and tracheal brushes was the least affected by infection route, disease progression, and individual differences. Therefore, SARS-CoV-2 nucleic acid detection using lower respiratory tract samples alone is reliable for COVID-19 diagnosis and study.

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“…Rodents exist in sufficient numbers and densities for continuous transmission and are often in close proximity to humans, but so far experimental studies indicate a low probability or no risk of SARS‐CoV‐2 infection for mice and rats 13 . Interestingly, it has been found more recently that Chinese tree shrews, a squirrel‐like mammal with a wide distribution in Southeast Asia, could not only be infected with SARS‐CoV‐2 but also developed clinical signs analogous to COVID‐19 in humans 15 . Chinese tree shrews have been used as animal models in viral hepatitis, psychosocial and visual defect studies due to their phylogenetical closeness to primates 16 …”

Section: Origin Of Sars‐cov‐2: What Have We Learned So Far?mentioning

confidence: 99%