COVID‐19: Laboratory/Serologic Diagnostics

Chin, Desmond Wai Loon; Eibschutz, Liesl S.; Sobczyk, Juliana; Azami, Mobin; Repajic, Michael Jovan; Dubé, Michael P.

doi:10.1002/9781119789741.ch3

Coronavirus Disease 2019 (COVID‐19) 2022

DOI: 10.1002/9781119789741.ch3

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COVID‐19: Laboratory/Serologic Diagnostics

Desmond Wai Loon Chin¹,

Liesl S. Eibschutz²,

Juliana Sobczyk³

et al.

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Cited by 2 publications

(1 citation statement)

References 132 publications

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“…Despite mass vaccination that led to a substantial reduction in the coronavirus disease 2019 (COVID-19), , the pandemic is ongoing. Therefore, there is an urgent need to prevent the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). − Rapid tests are used to identify and isolate SARS-CoV-2-infected individuals, enabling outbreak control. Several SARS-CoV-2 antigen rapid immunoassays have been developed. , However, the low sensitivity and specificity of immunoassays result in a higher incidence of false positives, thus limiting the application of these assays.…”

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confidence: 99%

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

Choi,

Song,

Cho

et al. 2023

Anal. Chem.

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The ongoing coronavirus disease 2019 (COVID-19) pandemic demands rapid and straightforward diagnostic tools to prevent early-stage viral transmission. Although nasopharyngeal swabs are a widely used patient sample collection method for diagnosing COVID-19, using these samples for diagnosis without RNA extraction increases the risk of obtaining false-positive and -negative results. Thus, multiple purification steps are necessary, which are time-consuming, generate significant waste, and result in substantial sample loss. To address these issues, we developed surface-modified polymerase chain reaction (PCR) tubes using the tertiary aminated polymer poly(2-dimethylaminomethylstyrene) (pDMAMS) via initiated chemical vapor deposition. Introducing the clinical samples into the pDMAMS-coated tubes resulted in approximately 100% RNA capture efficiency within 25 min, which occurred through electrostatic interactions between the positively charged pDMAMS surface and the negatively charged RNA. The captured RNA is then detected via chamber digital PCR, enabling a sensitive, accurate, and rapid diagnosis. Our platform provides a simple and efficient RNA extraction and detection strategy that allows detection from 22 nasopharyngeal swabs and 21 saliva specimens with 0% false negatives. The proposed method can facilitate the diagnosis of COVID-19 and contribute to the prevention of early-stage transmission.

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mentioning

confidence: 99%

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

Choi,

Song,

Cho

et al. 2023

Anal. Chem.

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Biosafety considerations for laboratory work with SARS-CoV-2

Mousavi,

Arefinia,

Sokhanvar

et al. 2024

Eur J Inflamm

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Today, the world is affected by a COVID-19 epidemic. Early diagnosis can play an important role in the control and prevention of this disease, so the study and practice of biosafety among staff of diagnostic laboratories have a key role in the healthiness of health fighters against COVID-19. In this study, we will review laboratory biosafety considerations for the diagnosis process of SARS-CoV-2 in three phases: (1) the preanalytical phase, (2) the analytical phase, and (3) the postanalytical phase. It should be noted that employees with high-risk underlying health conditions, like lung or heart disease or diabetes, should be moved to lower-risk environments. We believe that this review will help to restrict the risk of developing COVID-19, while working in clinical laboratories.

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COVID‐19: Laboratory/Serologic Diagnostics

Cited by 2 publications

References 132 publications

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

Streamlined Specimen Purification for Rapid COVID-19 Diagnosis Using Positively Charged Polymer Thin Film-Coated Surfaces and Chamber Digital PCR

Biosafety considerations for laboratory work with SARS-CoV-2

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