COVID-19 Diagnostic Strategies. Part I: Nucleic Acid-Based Technologies

Shaffaf, Tina; Ghafar-Zadeh, Ebrahim

doi:10.3390/bioengineering8040049

Cited by 23 publications

(15 citation statements)

References 110 publications

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“…In recent years, efforts have been made to develop instruments with dedicated fluorescence readout for isothermal amplification (e.g., Genie ® II by OptiGene). Similarly, the Lucira COVID-19 All-In-One Test Kit is an RT-LAMP-based platform that has recently been given FDA EUA and can be used for at-home testing of SARS-CoV-2 by people over 14 years of age [9]. However, these instruments are still relatively expensive because of the complex design.…”

Section: Introductionmentioning

confidence: 99%

A Portable Device for LAMP Based Detection of SARS-CoV-2

et al. 2021

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This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

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Section: Introductionmentioning

confidence: 99%

A Portable Device for LAMP Based Detection of SARS-CoV-2

et al. 2021

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“…This does suggest that some acute infections with higher Ct values (lower viral loads) could be missed. Differences in sensitivity between the two platforms could be due to the fact that BioFire RP2.1 is based on a nested PCR, which typically has higher sensitivity ( Shaffaf and Ghafar-Zadeh, 2021 ), or variation in gene targets or chemistries. Nevertheless, the results achieved for the QIAstat-Dx RP2.0 have concordance with the LOD described in the instruction manual.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the QIAstat-Dx RP2.0 and the BioFire FilmArray RP2.1 for the Rapid Detection of Respiratory Pathogens Including SARS-CoV-2

et al. 2022

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Point-of-care syndromic panels allow for simultaneous and rapid detection of respiratory pathogens from nasopharyngeal swabs. The clinical performance of the QIAstat-Dx Respiratory SARS-CoV-2 panel RP2.0 (QIAstat-Dx RP2.0) and the BioFire FilmArray Respiratory panel RP2.1 (BioFire RP2.1) was evaluated for the detection of SARS-CoV-2 and other common respiratory pathogens. A total of 137 patient samples were retrospectively selected based on emergency department admission, along with 33 SARS-CoV-2 positive samples tested using a WHO laboratory developed test. The limit of detection for SARS-CoV-2 was initially evaluated for both platforms. The QIAstat-Dx RP2.0 detected SARS-CoV-2 at 500 copies/mL and had a positive percent agreement (PPA) of 85%. The BioFire RP2.1 detected SARS-CoV-2 at 50 copies/mL and had a PPA of 97%. Both platforms showed a negative percent agreement of 100% for SARS-CoV-2. Evaluation of analytical specificity from a range of common respiratory targets showed a similar performance between each platform. The QIAstat-Dx RP2.0 had an overall PPA of 82% (67–100%) in clinical samples, with differences in sensitivity depending on the respiratory target. Both platforms can be used to detect acute cases of SARS-CoV-2. While the QIAstat-Dx RP2.0 is suitable for detecting respiratory viruses within a clinical range, it has less analytical and clinical sensitivity for SARS-CoV-2 compared to the BioFire RP2.1.

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“…Followed by Cas12 based cleavage of target sequence and visual detection on lateral flow reader. Reproduced from open-access article [ 65 ] under the terms of Creative Commons CC BY. …”

Section: Details On Covid-19mentioning

confidence: 99%

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Singh¹,

Datta²,

Ashish³

et al. 2021

Sensors International

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Without a doubt, the current global pandemic affects all walks of our life. It affected almost every age group all over the world with a disease named COVID-19, declared as a global pandemic by WHO in early 2020. Due to the high transmission and moderate mortality rate of this virus, it is also regarded as the panic-zone virus. This potentially deadly virus has pointed up the significance of COVID-19 research. Due to the rapid transmission of COVID-19, early detection is very crucial. Presently, there are different conventional techniques are available for coronavirus detection like CT-scan, PCR, Sequencing, CRISPR, ELISA, LFA, LAMP. The urgent need for rapid, accurate, and cost-effective detection and the requirement to cut off shortcomings of traditional detection methods, make scientists realize to advance new technologies. Biosensors are one of the reliable platforms for accurate, early diagnosis. In this article, we have pointed recent diagnosis approaches for COVID-19. The review includes basic virology of SARS-CoV-2 mainly clinical and pathological features. We have also briefly discussed different types of biosensors, their working principles, and current advancement for COVID-19 detection and prevention.

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COVID-19 Diagnostic Strategies. Part I: Nucleic Acid-Based Technologies

Cited by 23 publications

References 110 publications

A Portable Device for LAMP Based Detection of SARS-CoV-2

A Portable Device for LAMP Based Detection of SARS-CoV-2

Evaluation of the QIAstat-Dx RP2.0 and the BioFire FilmArray RP2.1 for the Rapid Detection of Respiratory Pathogens Including SARS-CoV-2

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

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