Cover Picture: Near‐Infrared Fluorescence Lifetime Imaging of Biomolecules with Carbon Nanotubes (Angew. Chem. Int. Ed. 24/2023)

Ratiometric Imaging of Catecholamine Neurotransmitters with Nanosensors

“…The possibility of tuning sensor properties on different levels can be used for a broad range of existing sensors to push their performance. More robust fluorescence imaging is also possible by NIR FLIM, as shown by us before . However, such approaches require optical setups capable of time-correlated single-photon counting (TCSPC), while ratiometric imaging can be performed in any wide-field fluorescence microscope.…”

“…However, movements of the sample or variations of the illumination bias such measurements. To circumvent this issue, self-calibrated approaches, such as fluorescence lifetime (FLIM) imaging, have been developed. , Another approach is to use fluorescent sensors or probes with at least two emission features. One emission peak serves as reference, and the second emission peak responds to the target analytes. − In the past, the advantages of ratiometric sensors have enabled, for example, improved calcium imaging.…”