“…For ESI, different technologies such as the RapidFire system in BLAZE-mode (Bretschneider et al, 2019) or ADE-OPI MS approach have been described for enzymatic-type assays (H€ abe et al, 2020;Simon et al, 2021a). The versatility of the instrument setup allows the analysis of many different biomolecules including lipids (Highkin et al, 2011;Dittakavi et al, 2020), peptides (Hutchinson et al, 2011;Liddle et al, 2020), and metabolites (Soulard et al, 2008;Maxine et al, 2009), from a wide range of matrix systems including blood, plasma (Highkin et al, 2011;Bretschneider et al, 2017) and cell lysates (Gordon et al, 2016;Dittakavi et al, 2020). Ambient ionization, such as desorption electrospray ionization (DESI), which commonly does not require sample preparation, is a new and attractive alternative for HT analysis.…”