Cover crops for nitrogen loss reductions: functional groups, species identity and traits

Biochemical Characterization of Novel GH6 Endoglucanase from Myxococcus sp. B6-1 and Its Effects on Agricultural Straws Saccharification

“…P33682.2). BLAST analysis manifested that CelA257 shared 54.7% of its identity with CelA1 from Streptomyces halstedii [18], followed by cusA from Streptomyces sp. KSM-9 (43.6%, P13933.3) [19], CenA from Cellulomonas fimi (43.3%, P07984.1) [20], E2 from Thermobifida fusca (40.8%, P26222.2) [21], and shared <40% of the sequence identity with other bacteria in GenBank database.…”

“…CelA257 contains a typical (β/α) 8-barrel structure of the GH6 family (Figure S1d), which includes endoglucanase (EC 3.2.1.4) [25], cellobiohydrolase (EC 3.2.1.91) [26], and lichenase/endo-β-1,3-1,4-glucanase (EC 3.2.1.73) [14]. CelA257 active center was composed of three aspartic acid residues (Figure S1e,f), with Asp441 acting as the catalytic nucleophile and Asp294 as a proton donor [18]. The deduced N-terminal binding domain was analyzed as a member of the CBM4-9 family.…”

Assessing the Effect of Intercropped Leguminous Service Crops on Main Crops and Soil Processes Using Apsim Ng

“…However, the scenario that was best in preventing N losses varied depending on crop period, especially for leaching losses, which were lower in the SC scenario in winter wheat but higher in the SC scenario during the fallow period. Studies in the field (De Notaris et al, 2018;Vogeler et al, 2019b) and in microcosms (Fernandez Pulido et al, 2023) have shown that living leguminous SCs reduce NO 3 leaching compared with bare soil. However, when leguminous SCs die off and decay, the SMN pool immediately starts to increase (Amossé et al, 2013;Bergkvist et al, 2011), which may increase both leaching and gaseous emissions (Olofsson and Ernfors, 2022;Storr et al, 2021).…”