Covariations of cerebral blood volume and single neurons discharge during resting state and behavioral visual cognitive tasks in non-human primates

Claron, Julien; Provansal, Matthieu; Salardaine, Quentin; Tisssier, Pierre; Dizeux, Alexandre; Deffieux, Thomas; Picaud, Serge; Tanter, Mickaël; Arcizet, Fabrice; Pouget, Pierre

doi:10.1101/2022.06.20.496840

Cited by 3 publications

(4 citation statements)

References 56 publications

(72 reference statements)

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“…4F), with most of the errors occurring at the transitions between the two task states. This effect is likely due in part to the latency between the neural activity and resulting hemodynamic response ( 3 , 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…On the basis of power Doppler imaging, fUSI measures changes in cerebral blood volume within a several-centimeters field of view by detecting the backscattered echoes from moving red blood cells. These cerebral blood volume changes are correlated with single neuron activity and local field potentials via neurovascular coupling ( 3 , 4 ). The spatial precision of fUSI approaches 100 μm with a framerate of up to 10 Hz, allowing fUSI to detect the function of small populations of neurons ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional ultrasound imaging of human brain activity through an acoustically transparent cranial window

Rabut,

Norman,

Griggs

et al. 2024

Sci. Transl. Med.

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Visualization of human brain activity is crucial for understanding normal and aberrant brain function. Currently available neural activity recording methods are highly invasive, have low sensitivity, and cannot be conducted outside of an operating room. Functional ultrasound imaging (fUSI) is an emerging technique that offers sensitive, large-scale, high-resolution neural imaging; however, fUSI cannot be performed through the adult human skull. Here, we used a polymeric skull replacement material to create an acoustic window compatible with fUSI to monitor adult human brain activity in a single individual. Using an in vitro cerebrovascular phantom to mimic brain vasculature and an in vivo rodent cranial defect model, first, we evaluated the fUSI signal intensity and signal-to-noise ratio through polymethyl methacrylate (PMMA) cranial implants of different thicknesses or a titanium mesh implant. We found that rat brain neural activity could be recorded with high sensitivity through a PMMA implant using a dedicated fUSI pulse sequence. We then designed a custom ultrasound-transparent cranial window implant for an adult patient undergoing reconstructive skull surgery after traumatic brain injury. We showed that fUSI could record brain activity in an awake human outside of the operating room. In a video game “connect the dots” task, we demonstrated mapping and decoding of task-modulated cortical activity in this individual. In a guitar-strumming task, we mapped additional task-specific cortical responses. Our proof-of-principle study shows that fUSI can be used as a high-resolution (200 μm) functional imaging modality for measuring adult human brain activity through an acoustically transparent cranial window.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functional ultrasound imaging of human brain activity through an acoustically transparent cranial window

Rabut,

Norman,

Griggs

et al. 2024

Sci. Transl. Med.

View full text Add to dashboard Cite

show abstract

“…6F), with most of the errors occurring at the transitions between the two task states. This effect is likely due in part to the latency between the neural activity and resulting hemodynamic response 24,25 .…”

Section: Acoustic Window Allows Fusi Activity Recording In Fully Reco...mentioning

confidence: 99%

A window to the brain: ultrasound imaging of human neural activity through a permanent acoustic window

Rabut

Norman

Griggs

et al. 2023

Preprint

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Recording human brain activity is crucial for understanding normal and aberrant brain function. However, available recording methods are either highly invasive or have relatively low sensitivity. Functional ultrasound imaging (fUSI) is an emerging technique that offers sensitive, large-scale, high-resolution neural imaging. However, fUSI cannot be performed through adult human skull. Here, we use a polymeric skull replacement material to create an acoustic window allowing ultrasound to monitor brain activity in fully intact adult humans. We design the window through experiments in phantoms and rodents, then implement it in a participant undergoing reconstructive skull surgery. Subsequently, we demonstrate fully non-invasive mapping and decoding of cortical responses to finger movement, marking the first instance of high-resolution (200μm) and large-scale (50mmx38mm) brain imaging through a permanent acoustic window.

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“…fUSI uses ultrafast Power Doppler Imaging (PDI) to measure changes in cerebral blood volume while suppressing signals from the surrounding tissue through the implementation of advanced spatiotemporal filtering techniques such as singular value decomposition (SVD) 26,27 . Analogous to fMRI, fUSI leverages neurovascular coupling and has been shown to correlate well with neuronal activity and local field potentials 28,29 . The spatial resolution is similar to that of fMRI 30 but it attains greater temporal resolution 16 .…”

Section: Introductionmentioning

confidence: 99%

Transcranial Functional Ultrasound Imaging Detects Focused Ultrasound Neuromodulation Induced Hemodynamic Changes in Mouse and Nonhuman Primate BrainsIn Vivo

Aurup,

Bendig,

Blackman

et al. 2024

Preprint

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Focused ultrasound (FUS) is an emerging noinvasive technique for neuromodulation in the central nervous system (CNS). To evaluate the effects of FUS-induced neuromodulation, many studies used behavioral changes, functional magnetic resonance imaging (fMRI) or electroencephalography (EEG). However, behavioral readouts are often not easily mapped to specific brain activity, EEG has low spatial resolution limited to the surface of the brain and fMRI requires a large importable scanner that limits additional readouts and manipulations. In this context, functional ultrasound imaging (fUSI) holds promise to directly monitor the effects of FUS neuromodulation with high spatiotemporal resolution in a large field of view, with a comparatively simple and flexible setup. fUSI uses ultrafast Power Doppler Imaging (PDI) to measure changes in cerebral blood volume, which correlates well with neuronal activity and local field potentials. We designed a setup that aligns a FUS transducer with a linear array to allow immediate subsequent monitoring of the hemodynamic response with fUSI during and after FUS neuromodulation. We established a positive correlation between FUS pressure and the size of the activated area, as well as changes in cerebral blood volume (CBV) and found that unilateral sonications produce bilateral hemodynamic changes with ipsilateral accentuation in mice. We further demonstrated the ability to perform fully noninvasive, transcranial FUS-fUSI in nonhuman primates for the first time by using a lower-frequency transducer configuration.

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Covariations of cerebral blood volume and single neurons discharge during resting state and behavioral visual cognitive tasks in non-human primates

Cited by 3 publications

References 56 publications

Functional ultrasound imaging of human brain activity through an acoustically transparent cranial window

Functional ultrasound imaging of human brain activity through an acoustically transparent cranial window

A window to the brain: ultrasound imaging of human neural activity through a permanent acoustic window

Transcranial Functional Ultrasound Imaging Detects Focused Ultrasound Neuromodulation Induced Hemodynamic Changes in Mouse and Nonhuman Primate BrainsIn Vivo

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