Covalent-organic framework nanobionics for robust cytoprotection

Liang, Jieying; Chen, Qianfan; Yong, Joel; Suyama, Hiroki; Biazik, Joanna; Njegic, Bosiljka; Rawal, Aditya; Liang, Kang

doi:10.1039/d3sc04973f

Cited by 1 publication

(1 citation statement)

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“…The immobilization of cells and enzymes separately has currently been extensively studied 21 – 24 , while the co-immobilization of enzymes and cells still confronts great challenges. Biocatalysis usually conducts in an aqueous solution with ions, which may result in the unintended dissociation of co-immobilized carriers that rely on weak interactions (e.g., calcium alginate or metal-organic frameworks, MOFs), leading to catalyst leakage and compromising system stability.…”

Section: Introductionmentioning

confidence: 99%

Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification

Zheng,

Tan

et al. 2024

Nat Commun

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Co-immobilization of cells and enzymes is often essential for the cascade biocatalytic processes of industrial-scale feasibility but remains a vast challenge. Herein, we create a facile co-immobilization platform integrating enzymes and cells in covalent organic frameworks (COFs) to realize the highly efficient cascade of inulinase and E. coli for bioconversion of natural products. Enzymes can be uniformly immobilized in the COF armor, which coats on the cell surface to produce cascade biocatalysts with high efficiency, stability and recyclability. Furthermore, this one-pot in situ synthesis process facilitates a gram-scale fabrication of enzyme-cell biocatalysts, which can generate a continuous-flow device conversing inulin to D-allulose, achieving space-time yield of 161.28 g L−1 d−1 and high stability (remaining >90% initial catalytic efficiency after 7 days of continuous reaction). The created platform is applied for various cells (e.g., E. coli, Yeast) and enzymes, demonstrating excellent universality. This study paves a pathway to break the bottleneck of extra- and intracellular catalysis, creates a high-performance and customizable platform for enzyme-cell cascade biomanufacturing, and expands the scope of biocatalysis process intensification.

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Section: Introductionmentioning

confidence: 99%