2019
DOI: 10.1667/rr15288.1
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Covalent Modification of Amino Acids and Peptides Induced by Ionizing Radiation from an Electron Beam Linear Accelerator Used in Radiotherapy

Abstract: To identify modifications to amino acids directly induced by ionizing radiation (IR), free amino acids and 3-residue peptides were exposed to radiation generated by a linear accelerator (Linac) used in radiotherapy. Mass spectrometry was performed to detail the relative sensitivity to radiation as well as identify covalent, IR-dependent adducts. The order of reactivity of the 20 common amino acids was generally in agreement with published literature except for His (most reactive of the 20) and Cys (less reacti… Show more

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Cited by 5 publications
(8 citation statements)
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“…These were combined and subjected to high-pH fractionation, and fractions were analyzed using MS (MS). Database searches on a whole proteome level focused on previously identified IR-induced amino acid modifications, including carbonylation (+13.98 Da), hydroxylation (+15.99 Da), dioxidation (+31.99 Da), and trioxidation (+47.99 Da) ( 25 , 29 ). We note here that although we classify +31.99 Da as dioxidation, such a mass shift could also be peroxidation.…”
Section: Resultsmentioning
confidence: 99%
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“…These were combined and subjected to high-pH fractionation, and fractions were analyzed using MS (MS). Database searches on a whole proteome level focused on previously identified IR-induced amino acid modifications, including carbonylation (+13.98 Da), hydroxylation (+15.99 Da), dioxidation (+31.99 Da), and trioxidation (+47.99 Da) ( 25 , 29 ). We note here that although we classify +31.99 Da as dioxidation, such a mass shift could also be peroxidation.…”
Section: Resultsmentioning
confidence: 99%
“…+47.99 Da on Cys results in a sulfonic acid chemical group, +13.98 Da on Pro can represent conversion to pyroglutamic acid, etc.). We additionally attempted to identify several previously reported, less-common modifications ( 25 , 29 , 30 ); however, none of these appeared to any significant degree in our data sets.…”
Section: Resultsmentioning
confidence: 99%
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“…To survey and quantify as much IR-induced oxidation as possible, peptides from ten replicates (five treated and five mock-treated) were analyzed using mass spectrometry (MS). The proteome databases were searched systematically for previously identified IR-induced amino acid modifications, including carbonylation (+13.98 Da), hydroxylation (+15.99 Da), dioxidation (+31.99 Da), and trioxidation (+47.99 Da) [ 26 , 36 ]. We note here that although we classify +31.99 Da as dioxidation, such a mass shift could also be peroxidation.…”
Section: Resultsmentioning
confidence: 99%