Covalent linking of haptens, proteins and nucleic acids to a modified polystyrene support

Proficiency testing for the detection of anti-citrullinated protein antibody in China

“…Conjugate may affect the stability of kit and occur nonspecific color [27]. Solid phase carrier may affect well-to-well variations and repeatability [28]. Chromogenic substrate may affect the background in detection [29].…”

Particle electrophoresis of micrometric‐sized superparamagnetic particles designed for magnetic purification of cells

“…First, steric-hindrance appears as one of the most rate-limiting factor for obtaining highly activated surfaces [6, 71 by limiting the surface density ligands and accessibility. Second, surface immobilization of molecules often reduces their biological activity either by covalent modification of active sites [18] or by conformational modifications [8,9]. The aim of this study is to apply particle electrophoresis [lo] to a qualitative and quantitative control for immobilization of proteins to the surface micrometric-sized superparamagnetic particles.…”

Time-Resolved Fluorescence Immunoassay of Thyroxine in Serum: Immobilized Antigen Approach

“…The determinations by the present method correlated well with these by the commonly used commercial DELFIA or RIA. Moreover, it is considered that the present immobilized antigen approach may be more suitable to assay haptens with a relatively low concentration in samples or to assay the free fraction of the haptens, because in these situations the required amount of the coated antigen can be easily realized with an appropriate hapten conjugate, or, more directly, by using the solid phase which can covalently bind the hapten (10,11).…”