Covalent linkage of recombinant hirudin to poly(ethylene terephthalate) (Dacron): creation of a novel antithrombin surface

Phaneuf, Matthew D.; Berceli, Scott A.; Bide, Martin; Quist, William G.; LoGerfo, Frank W.

doi:10.1016/s0142-9612(96)00193-7

Cited by 78 publications

(46 citation statements)

References 39 publications

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“…Again, PET fibres are mainly used in textile industry (Bhata and Naik 1984;Patel and Bal 2001). Apart from that, it is also used in the field of surgical polymeric textiles (Phaneuf et al 1997;Ferreira et al 2002;Metzger 2003), composites (Fu et al 2002), nanocomposites (Chang et al 2004), conducting polymer (Mishra et al 2000), electroactive polymer (Patel and Mallick 2000) etc. Polyesters are mainly cross-linked when irradiated (Kuleznev and Shershnev 1990).…”

Section: Introductionmentioning

confidence: 99%

Analysis of microstress in neutron irradiated polyester fibre by X-ray diffraction technique

2005

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Abstract. Microstresses developed in the crystallites of polymeric material due to irradiation of high-energy particle causes peak broadening and shifting of X-ray diffraction lines to lower angle. Neutron irradiation significantly changes the material properties by displacement of lattice atoms and the generation of helium and hydrogen by nuclear transmutation. Another important aspect of neutron irradiation is that the fast neutron can produce dense ionization at deep levels in the materials. The polyethylene terephthalate (PET) fibre of raw denier value, 78⋅ ⋅2, were irradiated by fast neutron of energy, 4⋅ ⋅44 MeV, at different fluences ranging from 1 × × 10 9 n/cm 2 to 1 × × 10 12 n/cm 2 . In the present work, the radiation heating microstresses developed in PET micro-crystallites was investigated applying X'Pert-MPD Philips Analytical X-ray diffractometer and the effects of microstresses in tensile strength of fibre measured by Instron have also been reported. The shift of 0⋅ ⋅45 cm -1 in the Raman peak position of 1614⋅ ⋅65 cm -1 to a higher value confirmed the development of microstresses due to neutron irradiation using micro-Raman technique. The defects due to irradiation were observed by SEM micrographs of single fibre for virgin and all irradiated samples.

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Section: Introductionmentioning

confidence: 99%

Analysis of microstress in neutron irradiated polyester fibre by X-ray diffraction technique

2005

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“…17,18 Briefly, VEGF was radiolabeled with 200 ci 125 I using an iodo-bead reaction for tracing purposes. I-VEGF-SH complex was purified by gel filtration using a PD-10 column.…”

Section: Vegf-albumin Complex Synthesismentioning

confidence: 99%

A biologically active VEGF construct in vitro: Implications for bioengineering‐improved prosthetic vascular grafts

Stone

Phaneuf

Sivamurthy

et al. 2001

J. Biomed. Mater. Res.

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Prosthetic arterial grafts are unable to develop an intact endothelial lining after implantation, predisposing them to fail. Strategies have been sought to enhance endothelialization using growth factors and cytokines. This study assessed the biologic activity of vascular endothelial growth factor (VEGF) covalently linked to bovine serum albumin (BSA). Native and modified VEGF were assayed for endothelial cell migration and proliferation. Migration assays were performed comparing the effects of 2% fetal bovine serum (FBS), 50 ng/mL, 100 ng/mL, and 200 ng/mL of native VEGF and VEGF-BSA. Proliferation assays were performed by using Alamar Blue comparing cellular growth in 1% FBS, 10% FBS, 100 ng/mL unbound VEGF, and 100 ng/mL VEGF-BSA. VEGF is a potent chemotactic agent for endothelial cells in both unbound and bound states. Native VEGF solutions (50 ng/mL, 100 ng/mL, and 200 ng/mL) stimulated 23.9 cells/high power field (HPF), 35.3 cells/HPF, and 49.1 cells/HPF (p < 0.005). VEGF-BSA solutions stimulated 25.9 cells/HPF, 39.1 cells/HPF, and 69.0 cells/HPF (p < 0.001). VEGF-BSA and native VEGF supported similar increased cellular proliferation compared with 1% FBS media (p < 0.002). Modified VEGF retains its chemotactic and proliferative properties in vitro. These findings suggest that bare prosthetic surfaces lined with VEGF bound to a "basecoat" albumin may support endothelial cell proliferation and migration and thereby offer new strategies to improve graft patency.

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“…Hirudin, a highly potent and specific natural thrombin inhibitor [8], has also been explored as a biomaterials coating but its low biological activity and stability after surface immobilization limits its utilization for short-term applications [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Selective albumin-binding surfaces modified with a thrombin-inhibiting peptide

Freitas¹,

Maia²,

Figueiredo³

et al. 2014

Acta Biomaterialia

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a b s t r a c tBlood-contacting medical devices have been associated with severe clinical complications, such as thrombus formation, triggered by the activation of the coagulation cascade due to the adsorption of certain plasma proteins on the surface of biomaterials. Hence, the coating of such surfaces with antithrombotic agents has been used to increase biomaterial haemocompatibility. Biomaterial-induced clotting may also be decreased by albumin adsorption from blood plasma in a selective and reversible way, since this protein is not involved in the coagulation cascade. In this context, this paper reports that the immobilization of the thrombin inhibitor D-Phe-Pro-D-Arg-D-Thr-CONH 2 (fPrt) onto nanostructured surfaces induces selective and reversible adsorption of albumin, delaying the clotting time when compared to peptide-free surfaces. fPrt, synthesized with two glycine residues attached to the N-terminus (GGfPrt), was covalently immobilized onto self-assembled monolayers (SAMs) having different ratios of carboxylate-hexa(ethylene glycol)-and tri(ethylene glycol)-terminated thiols (EG6-COOH/EG3) that were specifically designed to control GGfPrt orientation, exposure and density at the molecular level. In solution, GGfPrt was able to inactivate the enzymatic activity of thrombin and to delay plasma clotting time in a concentration-dependent way. After surface immobilization, and independently of its concentration, GGfPrt lost its selectivity to thrombin and its capacity to inhibit thrombin enzymatic activity against the chromogenic substrate n-p-tosyl-Gly-Pro-Arg-p-nitroanilide. Nevertheless, surfaces with low concentrations of GGfPrt could delay the capacity of adsorbed thrombin to cleave fibrinogen. In contrast, GGfPrt immobilized in high concentrations was found to induce the procoagulant activity of the adsorbed thrombin. However, all surfaces containing GGfPrt have a plasma clotting time similar to the negative control (empty polystyrene wells), showing resistance to coagulation, which is explained by its capacity to adsorb albumin in a selective and reversible way. This work opens new perspectives to the improvement of the haemocompatibility of blood-contacting medical devices.

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Covalent linkage of recombinant hirudin to poly(ethylene terephthalate) (Dacron): creation of a novel antithrombin surface

Cited by 78 publications

References 39 publications

Analysis of microstress in neutron irradiated polyester fibre by X-ray diffraction technique

Analysis of microstress in neutron irradiated polyester fibre by X-ray diffraction technique

A biologically active VEGF construct in vitro: Implications for bioengineering‐improved prosthetic vascular grafts

Selective albumin-binding surfaces modified with a thrombin-inhibiting peptide

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