Covalent immobilization of urease to modified ethyl cellulose

Akkaya, Alper

doi:10.1007/s12221-013-0022-x

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…Therefore, the microenvironment around the immobilized esterase was more acidic than that of the bulk solution. Other researchers have reported similar observations upon immobilization of esterase and other enzymes [10,17,23].…”

Section: Effect Of Ph and Temperature On The Free And Immobilized Estsupporting

confidence: 65%

“…From above results, it could be stated that an improvement in immobilization capacity and percent retained activity can be achieved by introducing fibrous polymer chains to the solid supports which could provide new insights into the design of novel support with different functional groups for immobilization of enzyme in various biotechnological applications. Compared with free enzyme in solution, immobilized enzyme can provide many advantages, such as reusability of immobilized enzyme, continuous operation in reactor, controlled product formation, and efficient processing [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…Magnetic particles can be easily recovered from the media by applying an external magnet [21][22][23]. When they are used for enzyme immobilization, particularly in enzyme reactors, this method facilitates the separation of enzymes from the reaction medium after the completion of the enzymatic reaction [16][17][18]. Thus, magnetic separations are relatively rapid and easy, requiring a simple apparatus compared with other separation methods such as centrifugation and filtration.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Mucor miehei esterase on core-shell magnetic beads via adsorption and covalent binding: Application in esters synthesis

Bayramoğlu

Arıca

2014

Fibers Polym

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In this work, poly(hydroxyethyl methacrylate/glycidylmethacrylate), p(HPMA-GMA), magnetic beads were prepared via suspension polymerization, and grafted with poly(methacrylic acid), p(MMAc) via redox polymerization. Esterase from M. miehei was immobilized onto core-shell magnetic beads with two different methods (i.e., adsorption and covalent binding). For adsorption, the effects of pH and initial esterase concentration on the immobilization efficiency via adsorption have been studied. The maximum adsorption capacity of the magnetic beads for esterase was found to be 27.6 mg/g magnetic beads at pH 7.0. The covalent immobilization of esterase via carbodiimide coupling on mp(HEMA-GMA)-gp(MAAc) beads was found to be the more effective than the direct immobilization of the enzyme on the mp(HEMA-GMA) beads. The covalently immobilized esterase on the mp(HEMA-GMA)-g-p(MAAc) beads exhibited higher activity compared to the adsorbed esterase on the same magnetic beads. Tributyrin hydrolysis properties of the free and both immobilized esterase preparations were determined under different experimental conditions. The operational, thermal and storage stabilities of the immobilized esterase preparations were increased compared with the free esterase. Finally, esterification reactions have been performed to produce citronellyl-esters (namely: acetate, butyrate and propionate) in n-hexane medium using covalently immobilized esterase. The immobilized esterase was effectively reused in successive batch runs in n-hexane medium for citronellyl-butyrate synthesis, and only 23 % activity was lost after 6 cycles.

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Section: Effect Of Ph and Temperature On The Free And Immobilized Estsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immobilization of Mucor miehei esterase on core-shell magnetic beads via adsorption and covalent binding: Application in esters synthesis

Bayramoğlu

Arıca

2014

Fibers Polym

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“…This has led to its application for the preparation of many different materials, ranging from the classic and established paper based systems to the more sophisticated gels (50), supports for catalysts (51,52) and enzymes (53,54), membranes (55) and electronics (56)(57)(58)(59).…”

Section: Functionalization Of Cellulosementioning

confidence: 99%

Amino Acid-Derived Imidazolium Zwitterions: Building Blocks for Renewable Ionic Liquids and Materials

Kirchhecker

Esposito

2014

ACS Symposium Series

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“…Nature cellulose is the most ancient and abundant biomass resource, with a trillion tons produced per year by photosynthesis . Because of the shortage of fossil fuel, the applications of cellulose have reattracted considerable attention both in academic and industry in recent years. − Cellulose acetate (CA), known as one of the most important cellulose derivatives, , has a wide range of applications, including textile, membrane separation, coatings, plastics, and cigarette industries and also food, medicine, filters, and living goods …”

Section: Introductionmentioning

confidence: 99%

Activation of Cellulose by Supercritical Tetrafluoroethane and Its Application in Synthesis of Cellulose Acetate

Chen

Cao

et al. 2015

Ind. Eng. Chem. Res.

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Cellulose is the most ancient and abundant polysaccharide on earth. However, the strong hydrogen bond networks and the high crystallinity strongly prevent cellulose from reacting with other compounds to produce higher performance materials. In this work, the investigation via molecular dynamics simulation showed that supercritical 1,1,1,2tetrafluoroethane (SC R134a) could effectively destroy the strong hydrogen bond networks of cellulose and activate cellulose. Furthermore, experiments were also conducted to support the simulation results, where cellulose was activated via introducing SC R134a and subsequently used in the synthesis of cellulose acetate. The experimental results revealed that SC R134a exhibited a prominent effect on activating cellulose. The crystallinity of cellulose decreased from 0.926 to 0.686 after being activated by SC R134a. In addition the morphology (SEM) of cellulose suggested that the average diameter increased by 25% and numerous cracks appeared on the outer surface of cellulose fibers. Moreover, the consumption of reagents for synthesizing cellulose acetate decreased sharply, especially for acetic acid, which was only 25% of that used in industry, therefore, making the process much more green and energy-saving.

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Covalent immobilization of urease to modified ethyl cellulose

Cited by 9 publications

References 26 publications

Immobilization of Mucor miehei esterase on core-shell magnetic beads via adsorption and covalent binding: Application in esters synthesis

Immobilization of Mucor miehei esterase on core-shell magnetic beads via adsorption and covalent binding: Application in esters synthesis

Amino Acid-Derived Imidazolium Zwitterions: Building Blocks for Renewable Ionic Liquids and Materials

Activation of Cellulose by Supercritical Tetrafluoroethane and Its Application in Synthesis of Cellulose Acetate

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