Covalent immobilization of liposomes on plasma functionalized metallic surfaces

Mourtas, Spyridon; Kastellorizios, Michail; Klepetsanis, Pavlos; Farsari, E.; Amanatides, E.; Mataras, D.; Pistillo, Bianca Rita; Favia, Pietro; Sardella, Eloisa; d’Agostino, Riccardo; Antimisiaris, Sophia G.

doi:10.1016/j.colsurfb.2011.01.002

Cited by 34 publications

(18 citation statements)

References 38 publications

(53 reference statements)

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“…In the last two decades, coatings functionalized with carbonyl, carboxylic, hydroxyl, amine, and ethylene oxide (CH 2 CH 2 O) ether groups have been extensively studied and proved to be appropriate for biomedical applications such as: protein, DNA, and biomolecules immobilization; cell adhesion and growth; biosensor fabrication; and non‐fouling surfaces …”

Section: Introductionmentioning

confidence: 99%

Deposition of Hydroxyl Functionalized Films by Means of Water Aerosol‐Assisted Atmospheric Pressure Plasma

Yang

Camporeale

Sardella

et al. 2014

Plasma Processes & Polymers

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The atmospheric pressure plasma deposition of hydroxyl functionalized hydrocarbon films is reported in this work, with a reactor fed with water aerosol and ethylene. The effects of power and feed flow rates onto film chemistry have been investigated. Coatings produced with this approach can find application in the biomedical field, among others, as platforms for cell adhesion and proliferation. Results show that operating at 4 kHz provides a much higher amount of hydroxyl group in the coating compared with samples obtained at 11 kHz. After water immersion, the stability of the films and their amount of hydroxyl groups remain high. A simplified deposition mechanism is proposed.

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Section: Introductionmentioning

confidence: 99%

Deposition of Hydroxyl Functionalized Films by Means of Water Aerosol‐Assisted Atmospheric Pressure Plasma

Yang

Camporeale

Sardella

et al. 2014

Plasma Processes & Polymers

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“…Among the numerous different functional groups, amine or carboxylic groups containing coatings could be synthesized using, for example, respectively allylamine and acrylic acid. [ 50,51 ] The developed coatings may be grafted with numerous types of proteins and are not specifi c to the immobilization of laccases and β-lactamases. The transposition for the grafting of other enzymes can lead to new durable surface functionalities, including antibacterial or antibiofi lm, for example, through the grafting of antimicrobial peptides and proteins.…”

Section: Long-term Storage Of Bioactive Surfacesmentioning

confidence: 99%

Self‐Defensive Coating for Antibiotics Degradation—Atmospheric Pressure Chemical Vapor Deposition of Functional and Conformal Coatings for the Immobilization of Enzymes

Bonot

Mauchauffé

Boscher

et al. 2015

Adv Materials Inter

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Conformal epoxy‐rich coatings are synthesized by plasma initiated chain‐growth polymerization of glycidyl methacrylate via a newly developed Plasma initiated chemical vapor deposition method at atmospheric pressure to provide a functional platform for the immobilization of enzymes degrading antibiotics (laccase and β‐lactamase). In addition to enhance the enzymes activity duration and intensity, surface immobilization is also leading to enzyme structure rigidification, allowing them to endure mechanical stresses generated by a laminar water flow of 30 km h−1, and this with no reduction of their enzymatic activity. Self‐defensive surface properties against microorganism's adhesion, preventing the enzyme alteration and improving the degradation performances, are obtained via surface saturation with Tween 20. The developed method is scaled up to high specific surface high‐density polyethylene biochips commonly used in water treatment, and shows self‐defensive abilities and particularly long lasting efficient degradation properties.

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“…Liposomes can www.intechopen.com be used as drug containers by encapsulation of therapeutic compounds, in some cases additionally targeted to their site of action by antibodies, and potentially offer a wide range of applications. Covalent coating of liposomes onto stainless steel was achieved via radiofrequency glow plasma assisted creation of a thin film of acrylic acid characterized by surface carboxylic groups to which the liposomes were attached via formation of amide bonds (Mourtas et al, 2011). While the study was considered by the authors to be a proof of principle, the presented method seems to be a versatile approach due to possible changes of process parameters for the liposome immobilization procedure as well as regarding the choice of different drugs for encapsulation.…”

Section: Regulation Of Drug Release By Barrier Layersmentioning

confidence: 99%