Covalent immobilization of glucose oxidase onto Poly(St-GMA-NaSS) monodisperse microspheres via BSA as spacer arm

Du, Tanxin; Liu, Bailing; Hou, Xiaohui; Zhang, Baotan; Du, Cuiming

doi:10.1016/j.apsusc.2009.04.172

Cited by 31 publications

(21 citation statements)

References 23 publications

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“…However, when poly glycine linker was used as the spacer for immobilization of α-chymotrypsin onto controlled pore glass and polyethylene glycol copolymer cross-linked with polyacrylamide, it was found that 2 glycines were the optimal linker length [25]. Introducing of appropriate spacer will change the unfavorable surface polarity of the carrier, causing the enzymatic denaturation and preventing the steric hindrance [26].…”

Section: Introductionmentioning

confidence: 99%

Rapid Immobilization of Cellulase onto Graphene Oxide with a Hydrophobic Spacer

Gao

Wang

et al. 2018

Catalysts

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A rapid immobilization method for cellulase was developed. Functional graphene oxide was synthesized and grafted with hydrophobic spacer P-β-sulfuric acid ester ethyl sulfone aniline (SESA) though etherification and diazotization. The functionalized graphene oxide was characterized by Fourier-transform infrared spectroscopy and was used as the carrier for the immobilization of cellulase via covalent binding. The immobilization of cellulase was finished in a very short time (10 min) and very high immobilization yield and efficiency of above 90% were achieved after optimization. When compared with the free cellulase, thermal and operational stabilities of the immobilized cellulase were improved significantly. At 50 °C, the half-life of the immobilized cellulase (533 min) was six-fold higher than that of the free cellulase (89 min). Additionally, the affinity between immobilized cellulase (Km = 2.19 g L −1 ) and substrate was more favorable than that of free cellulase (Km = 3.84 g L −1 ), suggesting the immobilized cellulase has higher catalytic efficiency. The possible immobilization mechanism was proposed. The results strongly indicate that the immobilization is highly efficient and has great potential for the immobilization of other enzymes.

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Section: Introductionmentioning

confidence: 99%

Rapid Immobilization of Cellulase onto Graphene Oxide with a Hydrophobic Spacer

Gao

Wang

et al. 2018

Catalysts

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“…Furthermore, as can be seen in Figure 3, increases in enzyme concentration resulted in increases in the amount of immobilized enzyme. Efficiency of immobilization in the optimized conditions was higher than those found by Du et al (2009), who reported a yield of 3.36% when GOD was immobilized in Poly (St-GMA-NaSS) microspheres with and without BSA-spacer. Figure 4a, b, c shows the response obtained in the biosensor for three independent glucose injections.…”

Section: God Immobilisationmentioning

confidence: 42%

Biosensor for determination of glucose in real samples of beverages

Lopes

Batista

et al. 2012

Food Sci. Technol

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A biosensor was developed for spectrophotometric determination of glucose concentrations in real samples of orange juice energetic drinks, and sport drinks. The biosensor consisted of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized onto polyaniline activated with glutaraldehyde (PANIG). Immobilization parameters were optimized for GOD, and maximum immobilization yield was 16% when 5.0 mg of PANIG and 8.9 U prepared in 0.1 mol.L-1 sodium phosphate buffer (pH 7.0) reacted for 60 minutes at 4 °C with gentle stirring. The linear operational range for glucose determination using optimized operational parameters was between 0.05 and 6.0 mg.mL-1 with a very good reproducibility of response. The results obtained in the biosensor were compared with those obtained using free enzymes (commercial kits) and then validated through statistical analysis using the Tukey test (95% confidence interval).

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“…Therefore, various investigations on the preparation of polyGMA and its modification have been reported [14][15][16][17][18][19]. Nastasovic et al reported the preparation of poly(GMA-co-EGDMA) modified with ethylene diamine and its metal sorption properties [14] and Unugur et al reported the preparation of polyGMA modified with 5-aminotetrasole and its proton conductivity [15].…”

Section: Introductionmentioning

confidence: 98%