Covalent immobilization of glucose oxidase on films prepared by electrochemical copolymerization of 3-methylthiophene and thiophene-3-acetic acid for amperometric sensing of glucose: Effects of polymerization conditions on sensing properties

Liu, Chuanjun; Kubo, Takashi; Yamazaki, Rekishu; Shimomura, Masato

doi:10.1016/j.eurpolymj.2007.06.005

Cited by 41 publications

(24 citation statements)

References 52 publications

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“…Therefore, the covalent methods have been applied to immobilize enzymes and have gained recognition. Immobilization of enzymes through covalent attachment has been demonstrated to prepare of stable enzyme derivatives [16][17][18][19][20][21][22][23][24][25]. The extent of these improvements may depend on other conditions of the system, i.e., the nature of the enzyme, the type of support, and the method of immobilization.…”

mentioning

confidence: 99%

Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization

Eldin

Seuror

Nasr

et al. 2010

Appl Biochem Biotechnol

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ρ-Benzoquinone-activated alginate beads were presented as a new carrier for affinity covalent immobilization of glucoamylase enzyme. Evidences of alginate modification were extracted from FT-IR and thermal gravimetric analysis and supported by morphological changes recognized through SEM examination. Factors affecting the modification process such as ρ-benzoquinone (PBQ) concentration, reaction time, reaction temperature, reaction pH and finally alginate concentration, have been studied. Its influence on the amount of coupled PBQ was consequently correlated to the changes of the catalytic activity and the retained activity of immobilized enzyme, the main parameters judging the success of the immobilization process. The immobilized glucoamylase was found kept almost 80% of its native activity giving proof of non-significant substrate, starch, diffusion limitation. The proposed affinity covalent immobilizing technique would rank among the potential strategies for efficient immobilization of glucoamylase enzyme.

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mentioning

confidence: 99%

Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization

Eldin

Seuror

Nasr

et al. 2010

Appl Biochem Biotechnol

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“…This binding eliminated undesired leaching of a toxic non-physiological mediator during glucose determination. In addition, GOx wired to a redox polymer improved the electrical contact between its redox center and the electrode surface afforded a non-diffusional route of electric communication between the GOx and the electrode surface was [129][130][131].…”

Section: Determination Of Glucosementioning

confidence: 98%

Functionalized polythiophenes: Recognition materials for chemosensors and biosensors of superior sensitivity, selectivity, and detectability

Huynh

Sharma

Sosnowska

et al. 2015

Progress in Polymer Science

121

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“…A sensitivity of 45 nA mmol − 1 l − 1 was achieved with the same sensor over a period of 30 days, thus demonstrating a high stability and suitability for long -term glucose measurements. The infl uence of nanostructured polythiophene morphology in amperometric glucose sensing was also investigated by Liu et al [97] . They produced nanostructured fi lms by electrochemical copolymerization of 3 -methylthiophene and thiophene -3 -acetic acid, and found that once the copolymer fi lm grew over a critical thickness, a spontaneous formation of nanostructures occurred, probably depending on changes in the chain length of deposited oligomers and formation of a branched structure as the fi lm thickened.…”

Section: Conductive Polymer Nanostructures Used In Catalytic Biosensorsmentioning

confidence: 99%

New Micro‐ and Nanotechnologies for Electrochemical Biosensor Development

Berti

Turner

2011

Biosensor Nanomaterials

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IntroductionOver the last decade, great attention has been paid to the inclusion of newly developed nanomaterials such as nanowires, nanotubes, and nanocrystals in sensor devices. This can be attributed to the ability to tailor the size and structure, and hence the properties of nanomaterials, thus opening up excellent prospects for designing novel sensing systems and enhancing the performance of bioanalytical assays [1] . Considering that most biological systems, including viruses, membranes, and protein complexes, are naturally nanostructured materials and that molecular interactions take place on a nanometer scale, nanomaterials are intuitive candidates for integration into biomedical and bioanalytical devices [2, 3] . Moreover, they can pave the way for the miniaturization of sensors and devices with nanometer dimensions (nanosensors and nanobiosensors) in order to obtain better sensitivity, specifi city, and faster rates of recognition compared to current solutions.The chemical, electronic, and optical properties of nanomaterials generally depend on both their dimensions and their morphology [4] . A wide variety of nanostructures have been reported in the literature for interesting analytical applications. Among these, organic and inorganic nanotubes, nanoparticles, and metal oxide nanowires have provided promising building blocks for the realization of nanoscale electrochemical biosensors due to their biocompatibility and technologically important combination of properties, such as high surface area, good electrical properties, and chemical stability. Moreover, the integration of nanomaterials in electrochemical devices offers the possibility of realizing portable, easy -to -use, and inexpensive sensors, due to the ease of miniaturization of both the material and the transduction system. Over the last decade, this fi eld has been extensively investigated and a huge number of papers have been published. This chapter principally summarizes progress made in the last few years (2005 to date) in the integration of nanomaterials such as carbon nanotubes (CNTs), nanoparticles, and polymer nanostructures in electrochemical biosensing systems.

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Covalent immobilization of glucose oxidase on films prepared by electrochemical copolymerization of 3-methylthiophene and thiophene-3-acetic acid for amperometric sensing of glucose: Effects of polymerization conditions on sensing properties

Cited by 41 publications

References 52 publications

Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization

Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization

Functionalized polythiophenes: Recognition materials for chemosensors and biosensors of superior sensitivity, selectivity, and detectability

New Micro‐ and Nanotechnologies for Electrochemical Biosensor Development

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