Covalent immobilization of glucose oxidase to poly(O-amino benzoic acid) for application to glucose biosensor

Abstract

A biosensor for glucose utilizing glucose oxidase (GOX) covalently coupled to poly(o‐amino benzoic acid) (PAB; a carboxy‐group‐functionalized polyaniline) is described. Amperometric response measurements conducted via unmediated and mediated (with ferrocene carboxylic acid and tetrathiafulvalene) reoxidation of GOX show that glucose can be detected over a wide range of concentrations. An enzyme‐conducting polymer‐mediator model provides for better charge transport in a biosensor. The optimal response, obtained at pH 5.5 and 300 K, lies in the 1–40 mM range. A kinetic plot yields the value of the apparent Michaelis–Menten constant, Kmapp. The operational stability of the PAB‐based glucose biosensor was experimentally determined to be about 6 days. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 78: 662–667, 2000

Effect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response

“…sors. A number of techniques, such as physical adsorption, [23] entrapment, [24] crosslinking, [25] and covalent bonding, [26] have been used to immobilize biological molecules in conducting polymers.…”

Biosensors' Preparation to Be Used for Entrapment Method With Copolymers Polyaniline Derivatives

“…The hydrogen peroxide formed in the reaction of oxidation can be quantified by electrochemical methods and its amount is directly related with the sugar quantity. The immovilized enzymes in polypirrol have been carried out by several methods such as physical adsorption, covalent union, entrapment and electrochemical doping [2][3][4][5][6][7][8][9] . The entrapment method involves to capture the enzyme during the polymer electrosynthesis process, from a monomeric solution that contains the enzyme.…”

“…This method presents the following requirements, the process of polymerization should be carried out in aqueous medium and pH that no deactivate or denature the enzyme, the monomer should be soluble in aqueous media and it's desirable that the polymer exhibits conductivity properties. Because to the previous requirements, polyaniline and polyaniline derivatives have not been studied enough using the entrapment method because: i) the monomer is practically insoluble at neutral pH in aqueous medium, ii) the polymer prepared at pH > 3.5, is non-conducting, and at pH < 3 the enzyme is de-naturalized 9 , iii) the polymer can be electrochemically synthesized in strong acid medium, nevertheless, the acid absorbed in the resulting polymer denatures the enzyme 4,9,[12][13][14] . Therefore, the entrapment method using aniline as monomer can not be used.…”

Ultrasensitive DNA hybridization biosensor based on polyaniline

“…Various types of biosensors including DNA hybridization biosensors using different forms of carbon or conducting polymers have been reported (Gerard et al, 1999;Ramanathan et al, 2000;Wang and Kawde, 2001;Riccardi et al, 2006;Youssoufi and Markrouf, 2002;Erdem et al, 2000). DNA adsorbed carbon paste electrode has been reported for hybridization detection of 15 ppm of complementary target of hepatitis B virus in presence of methylene blue as hybridization indicator using differential pulse voltammetry and cyclic voltammetry (Erdem et al, 2000).…”