Covalent immobilization of carbonic anhydrase on amine functionalized alumino-Siloxane aerogel beads for biomimetic sequestration of CO2

Shamna, I.; Jeong, Soon Kwan; Bhagiyalakshmi, Margandan

doi:10.1016/j.jiec.2021.05.010

Cited by 16 publications

(5 citation statements)

References 51 publications

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“…This outcome was quite consistent with most of the cases reported in the literature, in which the immobilized enzyme exhibited an increased optimal temperature compared to the free enzyme. Furthermore, the optimal temperatures reported for free CA and CA immobilized on alumina siloxane hydrogel beads were, respectively, 25 °C and 30 °C 41 . Similarly, the highest temperatures for free lipase and lipase hybrid nanoflowers have been reported to be 30 °C and 40 °C, respectively 42 .…”

Section: Resultsmentioning

confidence: 95%

“…Furthermore, the optimal temperatures reported for free CA and CA immobilized on alumina siloxane hydrogel beads were, respectively, 25 °C and 30 °C. 41 Similarly, the highest temperatures for free lipase and lipase hybrid nanoflowers have been reported to be 30 °C and 40 °C, respectively. 42 On the other hand, the ideal temperature for free CA and bimetallic CA hybrid nanoflowers was determined to be 56 °C.…”

Section: Resultsmentioning

confidence: 98%

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Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Sharma

Nadda

2023

J of Chemical Tech & Biotech

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BACKGROUND: Carbonic anhydrase (CA) is one of the most widely distributed enzymes among plants, animals, and microorganisms, and it has an enormous ability to capture carbon dioxide (CO 2 ). However, the real-time application of CA is still a challenge due to its low operational stability, its difficulty in recovery from the reaction medium, and its poor durability.RESULTS: The synthesis of insoluble protein inorganic hybrid structures at nanoscale was proven as quite useful to catalyze enzymatic biotransformation. Here, CA nanoflowers (CANF) were synthesized with the self-assembly of metal phosphate and CA. The synthesis of CANF was performed using 0.2 mg mL −1 protein and 2.0 mM CuSO 4 at 4 °C under mild shaking conditions. The CANF exhibited optimum activity at pH 7.5 and a temperature of 40°C. The synthesized CANF were used for CO 2 conversion under optimized conditions and their kinetic parameters were studied using p-NPA hydrolysis. The V max and K m of CANF were 185.18 ∼mol min −1 mL −1 and 4.72 mM, compared with those of free CA, 166.66 ∼mol min −1 mL −1 and 5.12 mM, respectively. The stability of CANF has improved remarkably. The CANF made of metal ions and protein showed higher stability and enzyme activity than free enzymes. Furthermore, the CANF showed good reusability due to their mechanical properties and monodispersity. The production of CaCO 3 by CANF was 1.71-fold higher than that by free CA. CONCLUSION:The newly formed CANF showed flower-like morphology with good catalytic activity. This study demonstrated that CANF technology has a bright future in the conversion of CO 2 into CaCO 3 .

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 98%

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Sharma

Nadda

2023

J of Chemical Tech & Biotech

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“…Successful immobilization of CA (Russo et al, 2022) on dispersed solid particles through covalent attachment, physical adsorption, or matrix entrapment results in a more stable catalyst that can be confined inside the absorption unit and can be easily recovered (Xu et al, 2021;Shamna et al, 2021;Liu et al, 2018). Moreover, much larger enzyme loadings are permitted by immobilization, avoiding protein precipitation and foaming in high carbonate concentration solvents (Peirce et al, 2018).…”

Section: Co2 Reactive Absorption In Bicarbonate/carbonate Solutions P...mentioning

confidence: 99%

CO2 capture and utilization by biocatalytic synthesis of carboxylic acids: a process design study

Marra,

Russo,

Knuutila

et al. 2024

Chemical Engineering Science

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“…Covalent binding implies the formation of bonds between the groups of adequately functionalized supports and an enzyme. This is, by far, the most extended approach for immobilizing enzymes, particularly for CA [ 152 , 155 , 178 , 179 ]. Several protein functional groups can be used for this purpose.…”

Section: Improving Ca Performance: Enzyme Immobilizationmentioning

confidence: 99%

“…Free BCA and BCA-Al/Si-NH2 remained 80% of the activities, after ten days. BCA-Al/Si-NH 2 retained 89% of their enzyme activity up to 10 cycles [155] Amine-functionalized by co-deposition of polydopamine (PDA) and polyethyleneimine (PEI)…”

Section: Covalent Binding and Cross-linkingmentioning

confidence: 99%

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

et al. 2023

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Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool for reducing the concentration of this greenhouse gas in the atmosphere. Among the other enzymes, carbonic anhydrase (CA) and formate dehydrogenase (FDH) are two key biocatalysts suitable for this challenge, facilitating the uptake of carbon dioxide from the atmosphere in complementary ways. Carbonic anhydrases accelerate CO2 uptake by promoting its solubility in water in the form of hydrogen carbonate as the first step in converting the gas into a species widely used in carbon capture storage and its utilization processes (CCSU), particularly in carbonation and mineralization methods. On the other hand, formate dehydrogenases represent the biocatalytic machinery evolved by certain organisms to convert CO2 into enriched, reduced, and easily transportable hydrogen species, such as formic acid, via enzymatic cascade systems that obtain energy from chemical species, electrochemical sources, or light. Formic acid is the basis for fixing C1-carbon species to other, more reduced molecules. In this review, the state-of-the-art of both methods of CO2 uptake is assessed, highlighting the biotechnological approaches that have been developed using both enzymes.

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Covalent immobilization of carbonic anhydrase on amine functionalized alumino-Siloxane aerogel beads for biomimetic sequestration of CO2

Cited by 16 publications

References 51 publications

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

CO2 capture and utilization by biocatalytic synthesis of carboxylic acids: a process design study

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

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Covalent immobilization of carbonic anhydrase on amine functionalized alumino-Siloxane aerogel beads for biomimetic sequestration of CO2

Cited by 16 publications

References 51 publications

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO2 into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO2 into value added product

CO2 capture and utilization by biocatalytic synthesis of carboxylic acids: a process design study

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

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Product

Resources

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Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product