Covalent cross-links in polyampholytic chitosan fibers enhances bone regeneration in a rabbit model

Ghosh, Prahlad C.; Rameshbabu, Arun Prabhu; Das, Dipankar; Francis, Nimmy; Pawar, Harpreet Singh; Subramanian, Bhuvaneshwaran; Pal, Sagar

doi:10.1016/j.colsurfb.2014.11.031

Cited by 32 publications

(23 citation statements)

References 37 publications

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“…46 , 47 Polyampholytic chitosan fibers promoted proliferation and osteogenic differentiation of MSCs, as well as osseous tissue regeneration, in a rabbit model. 48 In our study, the use of a porous chitosan scaffold with hBMSCs promoted bone healing in a rat tibial defect model. Better bone formation was observed when SIRT7 knockdown hBMSCs were present in the scaffold.…”

Section: Discussionmentioning

confidence: 66%

Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway

Chen

Zhang

et al. 2017

Cell Death Dis

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Sirtuin 7 (SIRT7) is a NAD+-dependent deacetylase in the sirtuin family. In a previous study, human bone marrow mesenchymal stem cells (hBMSCs) with reduced SIRT7 activity were developed to evaluate the effect of SIRT7 on osteogenesis. SIRT7 knockdown significantly enhanced osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Additionally, SIRT7 knockdown upregulated β-catenin. The enhanced osteogenesis due to SIRT7 knockdown was partially rescued by a Wnt/β-catenin inhibitor. Furthermore, SIRT7 knockdown hBMSCs combined with a chitosan scaffold significantly promoted bone formation in a rat tibial defect model, as determined by imaging and histological examinations. These findings suggest that SIRT7 has an essential role in osteogenic differentiation of hBMSCs, partly by activation of the Wnt/β-catenin signaling pathway.

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Section: Discussionmentioning

confidence: 66%

Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway

Chen

Zhang

et al. 2017

Cell Death Dis

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“…The reagents used in crosslinking treatments involve traditional dialdehydes, epoxy compounds, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC)/N-hydroxyl succinimide (NHS), etc. [9][10][11]. Apart from the traditional crosslinking treatments, more recently, some nanocomposite chitosan scaffolds were found to possess increased stability in wet states.…”

Section: Introductionmentioning

confidence: 99%

Crosslinked and Dyed Chitosan Fiber Presenting Enhanced Acid Resistance and Bioactivities

2016

Polymers

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Abstract:The application of biodegradable chitosan fiber for healthy and hygienic textiles is limited due to its poor acid resistance in wet processing and poor antioxidant activity. In order to prepare chitosan fiber with good acid resistance and high antioxidant activity, chitosan fiber was first crosslinked by a water-soluble aziridine crosslinker, and then dyed with natural lac dye consisting of polyphenolic anthraquinone compounds. The main application conditions and crosslinking mechanism of the aziridine crosslinker, the adsorption mechanism and building-up property of lac dye on the crosslinked fiber, and the effects of crosslinking and dyeing on the antioxidant and antibacterial activities of chitosan fiber were studied. The crosslinked fiber exhibited greatly reduced weight loss in acidic solution, and possessed excellent acid resistance. Lac dye displayed a very high adsorption capability on the crosslinked fiber and a high utilization rate under weakly acidic medium. The Langmuir-Nernst isotherm was the best model to describe the adsorption behavior of lac dye, and Langmuir adsorption had great contribution to total adsorption. Lac dyeing imparted good antioxidant activity to chitosan fiber. Crosslinking and dyeing had no impact on the good inherent antibacterial activity of chitosan fiber.

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“…In fact, the presence of reactive amino side groups provides many possibilities of chemical bonds and crosslinking reactions; commonly, to stabilize CS hydrogels and to enhance their mechanical properties and biodegradability and/or to ameliorate cellular adhesion properties, chemical or ionic cross-linking methods (e.g., UV, ionic cross-linking) are employed [20]. For instance, chemical cross-linkers such as epoxy compounds [21], aldehydes (formaldehyde, glyceraldehyde, and glutaraldehyde) [22], and carbodiimides [23] or ionic cross-linkers such as sulfates, citrates, and phosphates [24,25] have been used to stabilize CS [17]. This paper is focused on the preparation and characterization of an innovative CS scaffold produced starting from chitosan hydrogel covalently cross-linked with genipin, a natural extract of Gardenia jasminoides Ellis [26].…”

Section: Introductionmentioning

confidence: 99%

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

Dimida

Barca

Cancelli

et al. 2017

International Journal of Polymer Science

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Genipin (GN) is a natural molecule extracted from the fruit of Gardenia jasminoides Ellis according to modern microbiological processes. Genipin is considered as a favorable cross-linking agent due to its low cytotoxicity compared to widely used cross-linkers; it cross-links compounds with primary amine groups such as proteins, collagen, and chitosan. Chitosan is a biocompatible polymer that is currently studied in bone tissue engineering for its capacity to promote growth and mineral-rich matrix deposition by osteoblasts in culture. In this work, two genipin cross-linked chitosan scaffolds for bone repair and regeneration were prepared with different GN concentrations, and their chemical, physical, and biological properties were explored. Scanning electron microscopy and mechanical tests revealed that nonremarkable changes in morphology, porosity, and mechanical strength of scaffolds are induced by increasing the cross-linking degree. Also, the degradation rate was shown to decrease while increasing the crosslinking degree, with the high cross-linking density of the scaffold disabling the hydrolysis activity. Finally, basic biocompatibility was investigated in vitro, by evaluating proliferation of two human-derived cell lines, namely, the MG63 (human immortalized osteosarcoma) and the hMSCs (human mesenchymal stem cells), as suitable cell models for bone tissue engineering applications of biomaterials.

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Covalent cross-links in polyampholytic chitosan fibers enhances bone regeneration in a rabbit model

Cited by 32 publications

References 37 publications

Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway

Knockdown of SIRT7 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the Wnt/β-catenin signaling pathway

Crosslinked and Dyed Chitosan Fiber Presenting Enhanced Acid Resistance and Bioactivities

Effects of Genipin Concentration on Cross-Linked Chitosan Scaffolds for Bone Tissue Engineering: Structural Characterization and Evidence of Biocompatibility Features

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