Covalent conjugation of mechanically stiff graphene oxide flakes to three-dimensional collagen scaffolds for osteogenic differentiation of human mesenchymal stem cells

Kang, Seokyung; Park, Jong Bo; Lee, Tae-Jin; Ryu, Seungoh; Bhang, Suk Ho; La, Wan-Geun; Noh, Myungkyung; Hong, Byung Hee; Kim, Byung Soo

doi:10.1016/j.carbon.2014.11.029

Cited by 115 publications

(79 citation statements)

References 37 publications

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“…The weight loss for Col–GO‐90 was observed in two steps. The primary step started from less than 50 to 110°C, which is owing to the decomposition of labile oxygen in GO, followed by the second step in the range of 110–420°C related to decomposition of Col . The corresponding percentage of weight loss for this scaffold was determined to be about 95.65%.…”

Section: Resultsmentioning

confidence: 93%

“…To fabricate Col–GO scaffolds, flakes were conjugated covalently with Col scaffolds. The covalent binding between the Col scaffold and GO flakes was formed by adding EDC to activate carboxyl groups (COOH) of GO for reacting amine groups (NH 2 ) of the Col scaffold . Optical micrographs of the prepared scaffolds showed that by increasing the concentration of dark GO flakes, the color of Col scaffold becomes darker (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For example, graphene has been used to reinforce polymethyl methacrylate and polyvinyl alcohol which significantly increased the modulus and rigidity of these composites for biological applications . Furthermore, it has been revealed in a study by Kang et al that covalently GO‐coated Col could provide higher stiffness than a Col matrix . In another study, it was shown that by using different concentrations of GO, the modulus of the GO‐hybrid gels could be tunable within a range of 8–24 kPa .…”

Section: Discussionmentioning

confidence: 99%

“…Different forms of graphene in terms of the number of layers, lateral dimensions, shapes, chemistry, as well as cell types could also interfere in its cytotoxicity . For instance, it was reported that GO flakes only exhibited cytotoxicity to human mesenchymal stem cells (MSCs) at concentrations higher than 100 μg/mL and sizes of 3.8 ± 0.4 μm . Another study indicated that incorporation of GO into Poly Lactic‐co‐Glycolic Acid (PLGA) nanofibers improved neurite outgrowth and branching of neuronal cells compared to cells on pure PLGA nanofibers.…”

Section: Discussionmentioning

confidence: 99%

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Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Norahan

Amroon

Ghahremanzadeh

et al. 2018

J Biomedical Materials Res

101

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The application of a cardiac patch over the epicardial surface has shown positive effects in protecting cardiac function postinfarction. Electroactive patches could enhance electrical signal propagation among cardiac cells. In the present study, an electrically active composite of collagen and graphene oxide (Col-GO) was fabricated as a cardiac patch. Col scaffolds were fabricated using a freeze-drying method and coated covalently with GO. Some scaffolds were also reduced by a reduction agent to restore the high conductivity of GO. GO was shown to be a single layer with suitable lateral size for biological application. The Col-GO scaffolds contained randomly oriented interconnected pores with appropriate pore sizes of 120-138 AE 8 μm. GO flakes were also well distributed in the pore walls. By increasing the GO concentration, the tensile strength of the scaffolds was enhanced from 75 kPa for Col-GO-5 to 162 kPa for Col-GO-90. Young modulus also followed the same trend. Electrical conductivity of the scaffolds was in the range of semi-conductive materials (~10 −4 S/m), which is suitable for cardiac tissue engineering applications. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated no toxic effects on human umbilical vein endothelial cells (HUVECs) after 96 h. Also, a 10-days degradation product of the samples was compatible with HUVECs. Reduced scaffolds supported neonatal cardiomyocyte adhesion and upregulated the expression of the cardiac genes, including Cx43, Actin4, and Trpt-2 than their nonconductive counterparts. The obtained results confirmed the angiogenic properties of reduced Go-containing materials for cardiovascular applications where angiogenesis plays an important role, especially postinfarction.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Norahan

Amroon

Ghahremanzadeh

et al. 2018

J Biomedical Materials Res

101

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“…(a) A schematic illustrating the preparation of GO-collagen scaffolds by covalent conjugation of GO flakes to the pores of sponge-like collagen scaffolds. The GO flakes were conjugated by covalent bonds between the carboxyl groups of the GO and the amine groups of the collagen scaffolds 104.…”

mentioning

confidence: 99%

Graphene and its nanostructure derivatives for use in bone tissue engineering: Recent advances

Shadjou

Hasanzadeh

2016

J Biomedical Materials Res

125

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Tissue engineering and regenerative medicine represent areas of increasing interest because of the major progress in cell and organ transplantation, as well as advances in materials science and engineering. Tissue-engineered bone constructs have the potential to alleviate the demand arising from the shortage of suitable autograft and allograft materials for augmenting bone healing. Graphene and its derivatives have attracted much interest for applications in bone tissue engineering. For this purpose, this review focuses on more recent advances in tissue engineering based on graphene-biomaterials from 2013 to May 2015. The purpose of this article was to give a general description of studies of nanostructured graphene derivatives for bone tissue engineering. In this review, we highlight how graphene family nanomaterials are being exploited for bone tissue engineering. Firstly, the main requirements for bone tissue engineering were discussed. Then, the mechanism by which graphene based materials promote new bone formation was explained, following which the current research status of main types of nanostructured scaffolds for bone tissue engineering was reviewed and discussed. In addition, graphenebased bioactive glass, as a potential drug/growth factor carrier, was reviewed which includes the composition-structuredrug delivery relationship and the functional effect on the tissue-stimulation properties. Also, the effect of structural and textural properties of graphene based materials on development of new biomaterials for production of bone implants and bone cements were discussed. Finally, the present review intends to provide the reader an overview of the current state of the graphene based biomaterials in bone tissue engineering, its limitations and hopes as well as the future research trends for this exciting field of science.

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Graphene Oxide: A Unique Nano‐Platform to Build Advanced Multifunctional Composites

Girão

Pinto

Bessa

et al. 2016

Advanced 2D Materials

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Covalent conjugation of mechanically stiff graphene oxide flakes to three-dimensional collagen scaffolds for osteogenic differentiation of human mesenchymal stem cells

Cited by 115 publications

References 37 publications

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Graphene and its nanostructure derivatives for use in bone tissue engineering: Recent advances

Graphene Oxide: A Unique Nano‐Platform to Build Advanced Multifunctional Composites

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