Covalent attachment of poly(allylamine hydrochloride) onto ordered silica foams

Meléndez‐Ortiz, H. Iván; Puente-Urbina, Bertha; León, Griselda Castruita‐de; Mercado-Silva, Jesús Alfonso; Saucedo‐Salazar, Esmeralda; Garcı́a-Cerda, L.A.

doi:10.1007/s10934-020-00870-8

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…Similar characteristic peaks of SA and Gel were also observed in previous studies. − All of the characteristic peaks of SA and gelatin were detected in the IR spectrum of the A 1 :G 0.5 composite hydrogel particles (purple spectrum), indicating the presence of these polymers. In the IR spectrum of pure PAH (blue spectrum), absorption peaks at 3400 cm –1 (combination of N–H stretch and O–H stretch), 2914 and 2860 cm –1 (asymmetric and symmetric C–H stretch), 1602 and 1508 cm –1 (asymmetric and symmetric N–H bending), and 999 cm –1 (C-halogen, Cl stretching) were observed. , Almost all of the spectral peaks of PAH appeared in the IR spectrum of the PAH-coated CA hydrogel particles (pink spectrum), confirming the deposition of PAH on the surface of the CA hydrogel particles.…”

Section: Resultssupporting

confidence: 59%

“…In the IR spectrum of pure PAH (blue spectrum), absorption peaks at 3400 cm −1 (combination of N−H stretch and O−H stretch), 2914 and 2860 cm −1 (asymmetric and symmetric C−H stretch), 1602 and 1508 cm −1 (asymmetric and symmetric N−H bending), and 999 cm −1 (C-halogen, Cl stretching) were observed. 65,66 Almost all of the spectral peaks of PAH appeared in the IR spectrum of the PAH-coated CA hydrogel particles (pink spectrum), confirming the deposition of PAH on the surface of the CA hydrogel particles. Proliferation Ability and Viability of Y1 and HT-22 Cells on Hydrogel Surfaces.…”

Section: Influence Of Concentrations Of Sa and Crosslinking Agent Cac...mentioning

confidence: 75%

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Fabrication and Morphological Control of Nonspherical Alginate Hydrogel Particles

Daradmare,

Son,

Lee

2023

Langmuir

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Section: Resultssupporting

confidence: 59%

Section: Influence Of Concentrations Of Sa and Crosslinking Agent Cac...mentioning

confidence: 75%

Fabrication and Morphological Control of Nonspherical Alginate Hydrogel Particles

Daradmare,

Son,

Lee

2023

Langmuir

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“…The mass loss below 200 C is because of dehydration . The decomposition of the PAH side chains starts at around 230 C followed by the sulfonate groups in PSS. , The loss at higher temperatures is attributed to the degradation of the main polymeric backbones. − …”

Section: Results and Discussionmentioning

confidence: 99%

“…46 The decomposition of the PAH side chains starts at around 230°C followed by the sulfonate groups in PSS. [47][48] The loss at higher temperatures is attributed to the degradation of the main polymeric backbones. [47][48][49] Figure 2(A) shows the ATR-FTIR spectra of the native and immobilized enzyme.…”

Section: Characterization Of the Immobilized Enzymementioning

confidence: 99%

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Improving β-Galactosidase-Catalyzed Transglycosylation Yields by Cross-Linked Layer-by-Layer Enzyme Immobilization

Alavijeh

Meyer

Gras

et al. 2020

ACS Sustainable Chem. Eng.

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The biotransformation of lactose into gut-bioactive glycans catalyzed by βgalactosidase can give economic value to lactose-rich side streams generated in the food or dairy industry. Herein, we study the immobilization of the commercially used β-galactosidase from Bacillus circulans onto silica particles using an enzyme immobilization technology involving a cross-linked layer-by-layer encapsulation method. The immobilized β-galactosidase was used for the synthesis of Nacetyllactosamine (LacNAc) as an important precursor for numerous bioactive compounds and a prebiotic in itself. Techniques including molecular analysis, enzyme activity determination, secondary structure analysis, thermodynamic characterization as well as the determination of thermal and operational stability were conducted to characterize the immobilized enzyme. Changes in the activity of the enzyme after immobilization were attributed to possible changes in electrostatic, covalent and protein-protein interactions. Immobilization significantly improved enzymatic LacNAc yield compared to the free enzyme. In turn, this improved the economics and the sustainability of the process. The immobilized enzyme encapsulated in multilayer films was significantly more stable in the presence of divalent cations and its thermostability also substantially increased, with the thermal denaturation activation energy increasing from 53 kJ mol -1 to 294 kJ mol -1 . The immobilized enzyme was successfully reused in eight consecutive reaction cycles with no significant reduction in the LacNAc yield. The improved transgalactosylation yield and productivity, higher stability and reusability obtained with this immobilization method provide new opportunities for industrial applications.

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Surface charge engineering for structural control of colloidal silica rod-decorated silica particles and their flexible integration into magnetic core–multishell structures

Kang,

Kim,

Kim

et al. 2024

Applied Surface Science

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Covalent attachment of poly(allylamine hydrochloride) onto ordered silica foams

Cited by 7 publications

References 33 publications

Fabrication and Morphological Control of Nonspherical Alginate Hydrogel Particles

Fabrication and Morphological Control of Nonspherical Alginate Hydrogel Particles

Improving β-Galactosidase-Catalyzed Transglycosylation Yields by Cross-Linked Layer-by-Layer Enzyme Immobilization

Surface charge engineering for structural control of colloidal silica rod-decorated silica particles and their flexible integration into magnetic core–multishell structures

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