Course of Infection and Humoral Response to Leishmania major in Inbred Meriones unguiculatus

Ja, Yates; Ek, Saliba; Kl, Yates; Schmitz, Kathleen A.; Othman, M.; Gi, Higashi

doi:10.2307/3282874

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…6C. The time course of the response is similar to that after experimental viral infection (34) or parenteral parasitic infection with Dipetalonema viteae or Leishmania major, where elevated antibody levels occur in serum after 1 week of infection (1,58). The early peak in the IgM response is similar to that observed in a human volunteer who ingested H. pylori (44), although the secondary rise differs; long-term studies should indicate whether there is parallelism with phenomena in humans.…”

Section: Discussionmentioning

confidence: 54%

Experimental Infection of Mongolian Gerbils with Wild-Type and Mutant Helicobacter pylori Strains

et al. 1998

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Experimental Helicobacter pylori infection was studied in Mongolian gerbils with fresh human isolates that carry or do not carry cagA (cagA-positive orcagA-negative, respectively), multiply passaged laboratory strains, wild-type strain G1.1, or isogenic ureA,cagA, or vacA mutants of G1.1. Animals were sacrificed 1 to 32 weeks after challenge, the stomach was removed from each animal for quantitative culture, urease test, and histologic testing, and blood was collected for antibody determinations. No colonization occurred after ≥20 in vitro passages of wild-type strain G1.1 or with the ureA mutant of G1.1. In contrast, infection occurred in animals challenged with wild-type G1.1 (99 of 101 animals) or the cagA (25 of 25) or vacA (25 of 29) mutant of G1.1. Infection with G1.1 persisted for at least 8 months. All 15 animals challenged with any of three fresh humancagA-positive isolates became infected, in contrast to only 6 (23%) of 26 animals challenged with one of four fresh humancagA-negative isolates (P < 0.001). Similar to infection in humans, H. pylori colonization of gerbils induced gastric inflammation and a systemic antibody response to H. pylori antigens. These data confirm the utility of gerbils as an animal model of H. pylori infection and indicate the importance of bacterial strain characteristics for successful infection.

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Section: Discussionmentioning

confidence: 54%

Experimental Infection of Mongolian Gerbils with Wild-Type and Mutant Helicobacter pylori Strains

et al. 1998

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“…Eight additional Meriones spp. have been positively incriminated as reservoir hosts of L. major in different parts of this parasite's distribution [13], [38], [43], [44], [45]. Therefore, finding M. tristrami naturally infected with L. major was not surprising.…”

Section: Discussionmentioning

confidence: 99%

A Newly Emerged Cutaneous Leishmaniasis Focus in Northern Israel and Two New Reservoir Hosts of Leishmania major

Faiman¹,

Abbasi²,

Jaffe³

et al. 2013

PLoS Negl Trop Dis

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In 2006/7, 18 cases of cutaneous leishmaniasis (CL) were reported for the first time from Sde Eliyahu (pop. 650), a village in the Beit She'an valley of Israel. Between 2007–2011, a further 88 CL cases were diagnosed bringing the total to 106 (16.3% of the population of Sde Eliyahu). The majority of cases resided in the south-western part of the village along the perimeter fence. The causative parasite was identified as Leishmania major Yakimoff & Schokhor, 1914 (Kinetoplastida: Trypanosomatidae). Phlebotomus papatasi (Scopoli), 1786 (Diptera: Psychodidae) was found to be the most abundant phlebotomine species comprising 97% of the sand flies trapped inside the village, and an average of 7.9% of the females were positive for Leishmania ITS1 DNA. Parasite isolates from CL cases and a sand fly were characterized using several methods and shown to be L. major. During a comprehensive survey of rodents 164 Levant voles Microtus guentheri Danford & Alston, 1880 (Rodentia: Cricetidae) were captured in alfalfa fields bordering the village. Of these 27 (16.5%) tested positive for Leishmania ITS1 DNA and shown to be L. major by reverse line blotting. A very high percentage (58.3% - 21/36) of Tristram's jirds Meriones tristrami Thomas, 1892 (Rodentia: Muridae), found further away from the village also tested positive for ITS1 by PCR. Isolates of L. major were successfully cultured from the ear of a wild jird found positive by ITS1 PCR. Although none of the wild PCR-positive voles exhibited external pathology, laboratory-reared voles that were infected by intradermal L. major inoculation, developed patent lesions and sand flies became infected by feeding on the ears of these laboratory-infected voles. This is the first report implicating M. guentheri and M. tristrami as reservoirs of Leishmania. The widespread co-distribution of M. guentheri and P. papatasi, suggests a significant threat from the spread of CL caused by L. major in the Middle East, central Asia and southern Europe.

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“…to experimental infection with L. major : Yates et al (1989) found that the humoral response of M. unguiculatus to inoculation with the parasites was dose-related. Using ELISA, Yates et al (1989) showed that antibody levels peaked 3 weeks post-inoculation in animals given 10 4 or 10 5 promastigotes each and 11 weeks post-inoculation in those inoculated with 10 3 each. The peak levels were highest in the jirds given the largest doses.…”

Section: Discussionmentioning

confidence: 99%

Humoral response of Meriones libycus to experimental infection with Leishmania major

Al-Younes

Saliba

Al-Khateeb

et al. 1999

Annals of Tropical Medicine And Parasitology

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The humoral responses of laboratory-reared jirds (Meriones libycus) to inoculation with various doses of Leishmania major were determined. The animals were inoculated intradermally with 10(2), 10(3), 10(5) or 10(7) promastigotes of a strain of L. major originally isolated from a Jordanian patient. The jirds were then bled at various intervals throughout the 26 weeks of the study, and the sera checked, by IFAT, for antibodies to homologous parasites. There were no detectable humoral responses in the animals inoculated with 10(2) promastigotes each or in parasite-free controls but a positive response was apparent in each of the other jirds. The animals given 10(3) promastigotes each required 3 months to become IFAT-positive whereas those given 10(5) and 10(7) parasites only needed 4 and 2 weeks, respectively. More than 50% of the animals inoculated with 10(3) parasites each developed strongly positive sera 2 months post-infection, whereas > 50% of the animals inoculated with 10(5) or 10(7) parasites each had strongly or very strongly positive sera 4 and 2 weeks post-inoculation, respectively. The data indicate that, in M. libycus inoculated with L. major, the time required for the humoral response to develop and its intensity are both dose-related.

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Course of Infection and Humoral Response to Leishmania major in Inbred Meriones unguiculatus

Cited by 4 publications

References 9 publications

Experimental Infection of Mongolian Gerbils with Wild-Type and Mutant Helicobacter pylori Strains

Experimental Infection of Mongolian Gerbils with Wild-Type and Mutant Helicobacter pylori Strains

A Newly Emerged Cutaneous Leishmaniasis Focus in Northern Israel and Two New Reservoir Hosts of Leishmania major

Humoral response of Meriones libycus to experimental infection with Leishmania major

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