Course of infection and development of immunity in experimental infection of mice with Listeria serotypes

koenig, C H Von; B, Heymer; H, Hof; Finger, H

doi:10.1128/iai.40.3.1170-1177.1983

Cited by 39 publications

(12 citation statements)

References 14 publications

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“…Numerous previous studies on the experimental infection of mice by L. monocytogenes strains have remarked on the occurrence of weakly virulent strains isolated from food and clinical samples, but the basis of the lowered virulence has not been systemically examined [14,15,20,21]. Here we show that, despite their intermediate level of virulence, primary infection with such naturally occurring strains afford protection to subsequent infection by more pathogenic variants.…”

Section: Discussionmentioning

confidence: 58%

“…The question arises as to what these protective antigens might be. Numer-ous immunization studies performed previously with non-pathogenic Listeria have demonstrated their inability to generate long-lasting protection [13][14][15]. Also, mice infected with listeriolysinnegative strains do not acquire resistance because such bacteria are rapidly eliminated from the infected host even if repeated doses at regular intervals were administered to mice [16].…”

Section: Discussionmentioning

confidence: 99%

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Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes

Chakraborty

1994

FEMS Immunology and Medical Microbiology

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Experimental infections of mice with strains of Listeria spp. isolated from contaminated food sources allowed discrimination of strains into those either exhibiting high, attenuated or low virulence. Compared to the highly virulent L. monocytogenes strain EGD, an attenuated strain such as L99 persisted for shorter times (5 versus 10 days) in the infected host. Using a tissue culture cell model of infection, we found that, although strain L99 was capable of accumulating actin like its virulent counterpart following invasion, it was unable to generate the polarized actin tails required for intracellular and cell-to-cell movement. Immunoblot analysis using specific antiserum to the ActA polypeptide, a molecule that is necessary for movement of the bacterium within the eucaryotic cell, indicated that a slightly truncated form of this polypeptide was produced in the L99 strain. Despite its reduced virulence, the attenuated strain L99 was just as effective in generating protection in immune mice as the highly virulent strains, albeit with a 1000-fold higher infective dose. Based on the results obtained from this study, we suggest that one of the mechanisms accounting for widespread resistance in humans to infection by Listeria may be due to asymptomatic infections by naturally occurring strains attenuated for virulence.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes

Chakraborty

1994

FEMS Immunology and Medical Microbiology

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“…The proliferation of the L. monocytogenes strains in the livers and spleens and the histopathology of these tissues reflected the typical course of listeric infection and development of a cellular immune response by 3 to 4 days postinoculation (1,8,20,26,28,52). The larger populations of SA in the livers than in the spleens are characteristic of listeriosis in pregnant mice (32), whereas in nonpregnant mice larger populations of L. monocytogenes are consistently recovered from the spleen (2,20,32).…”

Section: Discussionmentioning

confidence: 96%

“…Similarly, L. innocua is recovered from the livers and spleens of mice inoculated by i.p. and intravenous injection but is rapidly eliminated without evoking an immune response (20,52).…”

Section: Discussionmentioning

confidence: 99%

Determination of virulence of different strains of Listeria monocytogenes and Listeria innocua by oral inoculation of pregnant mice

Lammerding

Glass

Gendron‐Fitzpatrick

et al. 1992

Appl Environ Microbiol

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A pregnant mouse model was developed to follow the course of infection after peroral inoculation with six different strains of Listeria monocytogenes and one strain of Listeria innocua. Tissues were sampled and analyzed by microbiologic and histologic methods for 5 days postinoculation. In gnotobiotic pregnant BALB/c mice, L. monocytogenes Scott A (SA), serotype 4b, colonized the gastrointestinal tract, translocated to the livers and spleens of mice by day 1 postinoculation, and multiplied in these tissues until day 4. Infection of the placental tissues occurred by days 3 and 4 and was followed by infection of the fetuses. Little damage of colonic and cecal tissues was evident by histologic examination. Livers and spleens showed a cellular immune response; a similar immune response was not detected in the placentas or fetuses. A rough variant of L. monocytogenes SA which was as virulent as the parent strain in mice when injected intraperitoneally was less virulent perorally and did not consistently infect the fetuses. L. monocytogenes ATCC 19113, serotype 3a, did not colonize the gastrointet tract, nor was it isolatted fom any internal tssue. L. monocyn es -strains of serotpes 1/2a and 1/2b behaved like the SA strain in this mouse model. L. innocua colonized the gastrointestinal tract and translocated to the livers and spleens but did not survive in these organs and rapidly disappeared without infecting placental and fetal tissues. In comparison with gnotobiotic mice, conventional pregnant mice inoculated with L. monocytogenes strains showed less consistent infection. These results suggest that the gnotobiotic pregnant mouse is a useful model for detecting differences in virulence relating to colonization, invasiveness, and uteroplacental infection which cannot be detected by intraperitoneal inoculation of mice.

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“…These virulent Listeria strains are able to replicate within professional phagocytes, as demonstrated during in vitro and in vivo studies (10,13). In contrast, exposure to either apathogenic Listeria strains or nonviable virulent L. monocytogenes fails to promote a protective immune response (1,3,6,7,12,22,23) unless associated with an adjuvant (19)(20)(21). Furthermore, antigen preparations derived from listerias fail to induce acquired resistance as well.…”

mentioning

confidence: 99%

Induction of immunity with avirulent Listeria monocytogenes 19113 depends on bacterial replication

1988

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Events necessary for triggering the cell-mediated response to intracellular parasites are poorly understood. Here we show that extremely high doses of avirulent Listeria monocytogenes 19113 (>109) induce a modest and short-lived state of resistance in BALB/c mice. Induction of this protective state could not be achieved with nonviable bacteria and was blocked by inhibiting replication of viable L. monocytogenes 19113 through antibiotic treatment. The immune response was antigen specific and could be adoptively transferred with lymphoid cells. However, unlike the prototypic acquired cellular resistance induced by virulent Listeria strains, the protective response induced by L. monocytogenes 19113 was extremely short-lived, lasting less than 2 weeks, with a precipitous decline in the activity of the immune cells involved. An intervening in vitro culture period with concanavalin A greatly enhanced the activity of the adoptively transferred immune cells.

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Course of infection and development of immunity in experimental infection of mice with Listeria serotypes

Cited by 39 publications

References 14 publications

Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes

Naturally occurring virulence-attenuated isolates of Listeria monocytogenes capable of inducing long term protection against infection by virulent strains of homologous and heterologous serotypes

Determination of virulence of different strains of Listeria monocytogenes and Listeria innocua by oral inoculation of pregnant mice

Induction of immunity with avirulent Listeria monocytogenes 19113 depends on bacterial replication

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