Coupling solid-phase microextraction to liquid chromatography. A review

Abstract

Solid-phase microextraction (SPME) is a technique for extraction of organic compounds from gaseous, aqueous, and solid matrices. SPME is rapid and simple, ideal for automation and for in situ measurements, and no harmful solvents are needed. The principle of SPME involves equilibration of the analytes between the sample matrix and an organic polymeric phase coated on a fused-silica fiber. SPME is traditionally combined with analysis by gas chromatography (GC) and this combination has proved sensitive, accurate, and precise for quantitative analysis of different classes of volatile compound. More recently SPME has been coupled with liquid chromatography to widen its range of application to non-volatile and thermally unstable compounds also. This article reviews the status of SPME coupled with liquid chromatography. It focuses on different applications of the technique, e.g. environmental samples, biological fluids, and food samples, to show that SPME-HPLC has great potential in the analysis of a wide range of compounds in different matrices.

Application of solid phase microextraction on dental composite resin analysis

“…The analytical precision was the criterion for judgment, and the mobile phase solvent system was found to provide the most stable desorption efficiency and least deviation (<5.0% RSD) on triplicate analyses at a concentration of 10 g/mL for all three dentin components. The mobile phase is an appropriate solution for desorption and can be performed in the dynamic mode with a flowing mobile phase [22]. As a result, desorption solvent employed for sample analyses was the same as the mobile phase solvent system in this study.…”

Determination of the immunosuppressant mycophenolic acid in human serum by solid-phase microextraction coupled to liquid chromatography

“…In order to widen its range of application, SPME has been interfaced with HPLC [27]. A growing interest for SPME coupled to HPLC was observed, especially in the past few years, as demonstrated by a number of recently published papers [28]. In particular, a new SPME-HPLC-UV method for the determination of the mycophenolic acid in cheese samples [29] was recently developed in our laboratory.…”

Polymer monolith‐integrated multilayer poly(dimethylsiloxane) microchip for online microextraction and capillary electrophoresis

“…Solid-phase microextraction (SPME) has been introduced as an excellent method for sample purification and precondition, benefiting from its attractive features including high sensitivity, solventless extraction, simplicity and easy automation [10][11][12]. In early years, coated open tubular capillary [13] and packed column [14] were extensively used for SPME; however, the integration of the two techniques in microfluidics is, to a certain extent, limited by the insufficient surface area of coated capillary and difficult immobilization of packed microbeads or microspheres, respectively.…”