Coupling of selenate reduction and pyrrhotite oxidation by indigenous microbial consortium in natural aquifer

Li, Liuliu; Yan, Wenyue; Zhang, Baogang; Zhang, Han; Geng, Rongyue; Sun, Sijia; Guan, Xiaohong

doi:10.1016/j.watres.2023.119987

Cited by 7 publications

(3 citation statements)

References 88 publications

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“…A study showed that NADH dehydrogenases of Shewanella oneidensis MR-1 that can produce NADH contribute to the reduction of Pd (II) [ 17 ]. The NADH also contributes to Se (VI) bioreduction through regulating the electron transfer [ 24 ]. Secondly, reductase plays significant roles in a series of intracellular redox reactions and is involved in various electron transfer cycles.…”

Section: Resultsmentioning

confidence: 99%

The Pd (II) Reduction Mechanisms in Bacillus megaterium Y-4 Revealed by Proteomic Analysis

Chen,

Wang,

Chen

et al. 2024

Nanomaterials

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Many studies have been conducted on the microbial reduction of Pd (II) to palladium nanoparticles (Pd-NPs) due to the environmental friendliness, low cost, and the decreased toxicity of Pd (II) ions. In this study, we investigate the reduction mechanism of Pd (II) by Bacillus megaterium Y-4 through proteomics. The data are available via ProteomeXchange with identifier PXD049711. Our results revealed that B. megaterium Y-4 may use the endogenous electron donor (NAD(P)H) generated by nirB, tdh, and fabG and reductase to reduce Pd (II) to Pd-NPs. The expression levels of fabG, tdh, gudB, and rocG that generate NAD(P)H were further increased, and the number of reduced Pd-NPs was further increased with the exogenous electron donor sodium formate. Endogenous electron mediators such as quinones and flavins in B. megaterium Y-4 can further enhance Pd (II) reduction. The findings provided invaluable information regarding the reduction mechanism of Pd (II) by B. megaterium Y-4 at the proteome level.

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Section: Resultsmentioning

confidence: 99%

The Pd (II) Reduction Mechanisms in Bacillus megaterium Y-4 Revealed by Proteomic Analysis

Chen,

Wang,

Chen

et al. 2024

Nanomaterials

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“…Its V3–V4 region was amplified with primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). After amplification and purification with standard methods, it was sent to the Majorbio Technology Co., Ltd. (Shanghai, China) for the construction of Illumina libraries through 16S rRNA gene amplicon and metagenomic sequencing . The raw data had been submitted to the NCBI database as PRJNA975271 and PRJNA975288.…”

Section: Materials and Methodsmentioning

confidence: 99%

Deciphering Vanadium Speciation in Smelting Ash and Adaptive Responses of Soil Microorganisms

He,

Zhang,

Yan

et al. 2024

ACS Nano

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Abundant smelting ash is discharged during pyrometallurgical vanadium (V) production. However, its associated V speciation and resultant ecological impact have remained elusive. In this study, V speciation in smelting ash and its influence on the metabolism of soil microorganisms were investigated. Smelting ashes from V smelters contained abundant V (19.6− 115.9 mg/g). V(V) was the dominant species for soluble V, while solid V primarily existed in bioavailable forms. Previously unrevealed V nanoparticles (V-NPs) were prevalently detected, with a peak concentration of 1.3 × 10 13 particles/g, a minimal size of 136.0 ± 0.6 nm, and primary constituents comprising FeVO 4 , VO 2 , and V 2 O 5 . Incubation experiments implied that smelting ash reshaped the soil microbial community. Metagenomic binning, gene transcription, and component quantification revealed that Microbacterium sp. and Tabrizicola sp. secreted extracellular polymeric substances through epsB and yhxB gene regulation for V-NPs aggregation to alleviate toxicity under aerobic operations. The V K-edge X-ray absorption near-edge structure (XANES) spectra suggested that VO 2 NPs were oxidized to V 2 O 5 NPs. In the anaerobic case, Comamonas sp. and Achromobacter sp. reduced V(V) to V(IV) for detoxification regulated by the napA gene. This study provides a deep understanding of the V speciation in smelting ash and microbial responses, inspiring promising bioremediation strategies to reduce its negative environmental impacts.

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“…Amplification of the 16S rRNA gene took place through polymerase chain reaction (PCR) utilizing primers 338F (5′-ACTCCTACG GGAGGCAGCAG 3′) and 806R (5′-GGAC-TACHVGGGTWTC TAAT 3′), targeting the V4-V5 hyper-variable regions. Each sample underwent the preparation of a 20 mL PCR amplification mixture, following the ingredients specified in a prior method (L. Li et al, 2023;Zhou et al, 2011). MajorBio (Shanghai, China) conducted Illumina sequencing on the HiSeq2500 platform (San Diego, CA, USA), with quantification performed using the QuantiFluor™-ST microfluorometer (Promega, Madison, WI, USA) (Lu et al, 2023).…”

Section: Microbial Community Investigationmentioning

confidence: 99%

Unveiling Soil Microbiome Adaptation and Survival Strategy Under Vanadium Stress in Nationwide Mining Environments

Zhang,

Jiao,

Xing

et al. 2024

JGR Biogeosciences

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In the soils of vanadium (V) smelters, a diverse array of microorganisms relies on metabolic activities for survival amid stress. However, the characteristics and functions of soil microbiomes in V mining environments remain unexplored on a continental scale. This study thoroughly investigates the microbial diversity, community assembly, and functional potential of soil microbiome across 90 V smelters in China. Alpha diversity decreases significantly along the V gradient, with V emerging as the primary factor influencing community structure, followed by other environmental, climatic, and geographic factors. The null model reveals that V induces homogeneous selection, shaping co‐occurrence patterns and leading to increased number of positive associations, particularly with keystone genera such as f_Gemmatimonadaceae, Nocardioides, Micromonospora, and Rubrobacter under higher V concentrations (>559.6 mg/kg). Moreover, a metagenomic analysis yields 67 metagenome‐assembled genomes, unraveling the potential metabolic pathways of keystone taxa and their likely involvement in the V(V) reduction process. Nitrate and nitrite reductase (nirK, narG), and mtrABC are found to be taxonomically affiliated with Micromonospora. sp, FEN‐1250. sp, Nocardioides. sp, etc. Additionally, the reverse citric acid cycle (rTCA) likely serves as the primary carbon fixation pathway, synthesizing alternative energy for putative V reducers, highlighting a potentially synergistic relationship between autotrophic and heterotrophic processes that supports microbial survival. Our findings comprehensively uncover the driving forces behind soil community variation under V stress, revealing robust strategies possibly employed by indigenous microorganisms to mitigate the impact of V. These insights hold potential for applications in bioremediation.

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Coupling of selenate reduction and pyrrhotite oxidation by indigenous microbial consortium in natural aquifer

Cited by 7 publications

References 88 publications

The Pd (II) Reduction Mechanisms in Bacillus megaterium Y-4 Revealed by Proteomic Analysis

The Pd (II) Reduction Mechanisms in Bacillus megaterium Y-4 Revealed by Proteomic Analysis

Deciphering Vanadium Speciation in Smelting Ash and Adaptive Responses of Soil Microorganisms

Unveiling Soil Microbiome Adaptation and Survival Strategy Under Vanadium Stress in Nationwide Mining Environments

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