Coupling of liquid chromatograph with ion-mobility spectrometer

Kurnin, I. V.; Kayumov, Alexey A.; Muradymov, M. Z.; Krasnov, N. V.; Самокиш, А В

doi:10.1007/s12127-012-0110-4

Cited by 4 publications

(9 citation statements)

References 6 publications

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“…the detection of charged solvent clusters or nano droplets. In recent years, several groups succeeded in operating ESI-IM spectrometers at significantly higher flow rates by modifying the source region [11,12]. The highest flow rate at which stable operation could be shown, 1500 L/min, was achieved by our group [13].…”

Section: Introductionmentioning

confidence: 92%

“…Two substance mixtures consisting of 24 pesticides and 18 amino acids are used for the characterization of the IM detector. The following compounds are contained in the pesticide mixture (Supporting Information: Table S1): diquatdibromide(1), aldicarbsulfoxide(2), imidacloprid(3), aldicarbsulfone(4), methomyl(5), oxamyl(6), chloridazone (7), metoxurone(8), 3-hydroxycarbofurane (9), aldicarb(10), monurone (11), propoxur (12), diurone (13), chlortolurone (14), carbofuran (15), carbaryl (16), methabenzthiazurone(17), monolinurone (18), isoproturone (19), metobromurone (20), metazachlor (21), methiocarb (22), linurone(23) and metolachlor (24). The amino acid mixture is comprised of the following compounds: DL-methionine (Met), DL-valine (Val), L-glutamine (Gln), glutamic acid (Glu), glycine (Gly), L-histidine (His), L-alanine (Ala), L-arginine (Arg), L-aspartic acid (Asp), L-asparagine (Asn), L-cysteine (Cys), L-isoleucine (Ile), L-leucine (Leu), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-tyrosine (Tyr) and L-tryptophan (Trp).…”

Section: Chemicalsmentioning

confidence: 99%

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High‐performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications

Zühlke

Riebe

Beitz

et al. 2016

J of Separation Science

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The combination of high-performance liquid chromatography and electrospray ionization ion mobility spectrometry facilitates the two-dimensional separation of complex mixtures in the retention and drift time plane. The ion mobility spectrometer presented here was optimized for flow rates customarily used in high-performance liquid chromatography between 100 and 1500 μL/min. The characterization of the system with respect to such parameters as the peak capacity of each time dimension and of the 2D spectrum was carried out based on a separation of a pesticide mixture containing 24 substances. While the total ion current chromatogram is coarsely resolved, exhibiting coelutions for a number of compounds, all substances can be separately detected in the 2D plane due to the orthogonality of the separations in retention and drift dimensions. Another major advantage of the ion mobility detector is the identification of substances based on their characteristic mobilities. Electrospray ionization allows the detection of substances lacking a chromophore. As an example, the separation of a mixture of 18 amino acids is presented. A software built upon the free mass spectrometry package OpenMS was developed for processing the extensive 2D data. The different processing steps are implemented as separate modules which can be arranged in a graphic workflow facilitating automated processing of data.

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Section: Introductionmentioning

confidence: 92%

Section: Chemicalsmentioning

confidence: 99%

High‐performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications

Zühlke

Riebe

Beitz

et al. 2016

J of Separation Science

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“…Two attempts to increase the solvent flow rate were reported by the Eiceman group 15 and Kurnin et al 16 The Eiceman group was able to perform HPLC separation of chlorophenols on a C18 column at flow rates up to 500 µL min -1 and a water content of up to 80%. In these experiments, the ESI source was aligned axial in the spectrometer axis.…”

Section: Introductionmentioning

confidence: 99%

“…The authors designed a novel interface for the removal of solvents and worked at challenging instrument parameters. The work of Kurnin et al 16 presented a promising approach based on an additional ionization tube (T-cylinder) orthogonal to the spectrometer axis. The ESI was performed in this tube.…”

Section: Introductionmentioning

confidence: 99%

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An Electrospray Ionization-Ion Mobility Spectrometer as Detector for High-Performance Liquid Chromatography

Zühlke

Riebe

Beitz

et al. 2015

Eur J Mass Spectrom (Chichester)

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The application of electrospray ionization (ESI) ion mobility (IM) spectrometry on the detection end of a high-performance liquid chromatograph has been a subject of study for some time. So far, this method has been limited to low flow rates or has required splitting of the liquid flow. This work presents a novel concept of an ESI source facilitating the stable operation of the spectrometer at flow rates between 10 μL mn(-1) and 1500 μL min(-1) without flow splitting, advancing the T-cylinder design developed by Kurnin and co-workers. Flow rates eight times faster than previously reported were achieved because of a more efficient dispersion of the liquid at increased electrospray voltages combined with nebulization by a sheath gas. Imaging revealed the spray operation to be in a rotationally symmetric multijet mode. The novel ESI-IM spectrometer tolerates high water contents (≤90%) and electrolyte concentrations up to 10mM, meeting another condition required of high-performance liquid chromatography (HPLC) detectors. Limits of detection of 50 nM for promazine in the positive mode and 1 μM for 1,3-dinitrobenzene in the negative mode were established. Three mixtures of reduced complexity (five surfactants, four neuroleptics, and two isomers) were separated in the millisecond regime in stand-alone operation of the spectrometer. Separations of two more complex mixtures (five neuroleptics and 13 pesticides) demonstrate the application of the spectrometer as an HPLC detector. The examples illustrate the advantages of the spectrometer over the established diode array detector, in terms of additional IM separation of substances not fully separated in the retention time domain as well as identification of substances based on their characteristic Ims.

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